Determination of arbutin and bergenin in Bergeniae Rhizoma by capillary electrophoresis with a carbon nanotube-epoxy composite electrode

Zhang, Luyan; Zhang, Wei; Chen, Gang

doi:10.1016/j.jpba.2015.07.044

Cited by 11 publications

(5 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, they prepared a carbon nanotube (CNT)‐epoxy composite electrode for the CE‐AD analysis of two phenolic compounds, arbutin and bergenin in Bergeniae Rhizoma. The results indicated that CNTs in the composite significantly increased the current response of the electrode toward the analytes, with LODs of 0.21 and 0.23 μM for arbutin and bergenin, respectively . Similar work was reported by them for preparing CNT‐polydimethylsiloxane composite electrode for CE‐AD analysis of vanillin, ferulic acid, vanillic acid, caffeic acid and protocatechuic acid in Rhizoma Chuanxiong.…”

Section: Methods Developmentsupporting

confidence: 80%

Capillary electrophoresis in phytochemical analysis (2014–2017)

Zhou

Zhang

2018

Separation Science Plus

View full text Add to dashboard Cite

This updated review is focused on the applications of CE in phytochemical analysis during the period of 2014 to April 2018 (about 130 papers were included). The article mainly consists of three main sections, including sample pretreatment (extraction and on‐line pre‐concentration), method development (separation and detection) and applications in quality evaluation of medicinal plants, screening of active compounds (enzyme inhibitors) and studying interaction between phytochemicals and bio‐macromolecules.

show abstract

Section: Methods Developmentsupporting

confidence: 80%

Capillary electrophoresis in phytochemical analysis (2014–2017)

Zhou

Zhang

2018

Separation Science Plus

View full text Add to dashboard Cite

show abstract

“…Numerous methods have already been developed for the determination of arbutin in plant extracts, including spectrophotometry (18), capillary zone electrophoresis (19), GC-MS (20,21), and RP-HPLC (9,(22)(23)(24)(25). However, an efficient analytical method for the quantification of arbutin using LC-MS/MS has not been developed yet.…”

Section: Introductionmentioning

confidence: 99%

Development and validation of a modified QuEChERS method coupled with LC-MS/MS to determine arbutin in pear peels

Kim

Lee

2016

Food Sci Biotechnol

View full text Add to dashboard Cite

A new effective method was developed to determine the concentration of arbutin in pear peels using a modified QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) method and liquid chromatography-tandem mass spectrometry (LC-MS/MS). The original QuEChERS was modified to enable the extraction of the polar arbutin molecule. Use of an initial 50:50 acetonitrile:water extraction solvent led to the highest extraction efficiency. The arbutin extracted from pear peels was found to be identical to the β-arbutin standard, as confirmed by NMR and LC-MS/MS analyses. For quantitative analysis, the mass spectra of the precursor ion [M+NH] at / 290.0 and the product ion of arbutin at / 163.0 were used. The limit of detection, limit of quantification, linearity, precision, accuracy, and recovery of the proposed method were evaluated. We successfully applied this method to pear samples and it may be suitable for the quantitative analysis of arbutin in other similar plant materials.

show abstract

“…Some reports describe the fabrication and subsequent application of novel detection electrodes for determining the bioactive ingredients by CE, for instance in Belamcandae rhizome [ 27 ], in Bergeniae rhizoma [ 28 ] and in Cacumen platycladi [ 29 ]. In those cases, the values of LOD/LOQ were also as low as ng per mL −1 .…”

Section: Resultsmentioning

confidence: 99%

“…However, when it comes to analysis of trace analytes in a complex biological matrix, an introduction of extremely sensitive detectors, such as mass spectrometry or laser-induced fluorescence is recommended [26]. Some reports describe the fabrication and subsequent application of novel detection electrodes for determining the bioactive ingredients by CE, for instance in Belamcandae rhizome [27], in Bergeniae rhizoma [28] and in Cacumen platycladi [29]. In those cases, the values of LOD/LOQ were also as low as ng per mL −1 .…”

Section: Detection Sensitivitymentioning

confidence: 99%

Recent Applications of Capillary Electrophoresis in the Determination of Active Compounds in Medicinal Plants and Pharmaceutical Formulations

et al. 2021

View full text Add to dashboard Cite

The present review summarizes scientific reports from between 2010 and 2019 on the use of capillary electrophoresis to quantify active constituents (i.e., phenolic compounds, coumarins, protoberberines, curcuminoids, iridoid glycosides, alkaloids, triterpene acids) in medicinal plants and herbal formulations. The present literature review is founded on PRISMA guidelines and selection criteria were formulated on the basis of PICOS (Population, Intervention, Comparison, Outcome, Study type). The scrutiny reveals capillary electrophoresis with ultraviolet detection as the most frequently used capillary electromigration technique for the selective separation and quantification of bioactive compounds. For the purpose of improvement of resolution and sensitivity, other detection methods are used (including mass spectrometry), modifiers to the background electrolyte are introduced and different extraction as well as pre-concentration techniques are employed. In conclusion, capillary electrophoresis is a powerful tool and for given applications it is comparable to high performance liquid chromatography. Short time of execution, high efficiency, versatility in separation modes and low consumption of solvents and sample make capillary electrophoresis an attractive and eco-friendly alternative to more expensive methods for the quality control of drugs or raw plant material without any relevant decrease in sensitivity.

show abstract

Determination of arbutin and bergenin in Bergeniae Rhizoma by capillary electrophoresis with a carbon nanotube-epoxy composite electrode

Cited by 11 publications

References 19 publications

Capillary electrophoresis in phytochemical analysis (2014–2017)

Capillary electrophoresis in phytochemical analysis (2014–2017)

Development and validation of a modified QuEChERS method coupled with LC-MS/MS to determine arbutin in pear peels

Recent Applications of Capillary Electrophoresis in the Determination of Active Compounds in Medicinal Plants and Pharmaceutical Formulations

Contact Info

Product

Resources

About