Determination of antioxidant potential of<i>Acacia nilotica</i>leaf extract in oxidative stress response system of<i>Saccharomyces cerevisiae</i>

Subhaswaraj, Pattnaik; Sowmya, M.; Rajkumari, Jobina; Sudharshan, S. J.; Dyavaiah, Madhu; Siddhardha, Busi

doi:10.1002/jsfa.8409

Cited by 14 publications

(3 citation statements)

References 39 publications

(43 reference statements)

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“…In the current study, the antioxidant activity of A. nilotica flower extract was better than using other parts of the plant, as shown in comparison to some previous studies. For example, Subhaswaraj et al [57] studied the antioxidant activity A. nilotica leaf extract and showed an IC 50 of 75.157 µg/mL. Polyphenols from the extract of A. nilotica ripe fruits demonstrated an IC 50 of 41.91 µg/mL [42].…”

Section: Antioxidant Activity a Nilotica Flower Extractmentioning

confidence: 99%

Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico

Al-Rajhi

Qanash

Bazaid

et al. 2023

JFB

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The resistance of cancer and Helicobacter pylori to several drugs reflects a worldwide problem, and it has been the intention of numerous researchers to overcome this problem. Thus, in this study, Acacia nilotica fruits were subjected to HPLC analysis to detect their phenolic compounds and flavonoids. Moreover, A. nilotica‘s anti-H. pylori activity and its inhibitory activity against human hepatocellular carcinoma (HepG-2 cells) were reported. Various compounds with different concentrations, such as ferulic acid (5451.04 µg/mL), chlorogenic acid (4572.26 µg/mL), quercetin (3733.37 µg/mL), rutin (2393.13 µg/mL), gallic acid (2116.77 µg/mL), cinnamic acid (69.72 µg/mL), hesperetin (121.39 µg/mL) and methyl gallate (140.45 µg/mL), were detected. Strong anti-H. pylori activity at 31 mm was reported, compared to the positive control of the 21.67 mm inhibition zone. Moreover, the MIC and MBC were 7.8 µg/mL and 15.62 µg/mL, respectively, while the MIC and MBC of the positive control were 31.25 µg/mL. The concentration of MBC at 25%, 50% and 75% reflected H. pylori’s anti-biofilm activity of 70.38%, 82.29% and 94.22%, respectively. Good antioxidant properties of the A. nilotica flower extract were documented at 15.63, 62.50, 250 and 1000 µg/mL, causing the DPPH scavenging percentages of 42.3%, 52.6%, 65.5% and 80.6%, respectively, with a IC50 of 36.74 µg/mL. HepG-2 cell proliferation was inhibited (91.26%) using 500 µg/mL of flower extract with an IC50 of 176.15 µg/mL, compared to an IC50 of 395.30 µg/mL used against human normal melanocytes. Molecular docking was applied to investigate ferulic acid with the H. pylori (4HI0) crystal structure to determine the best binding mode that interacted most energetically with the binding sites. Molecular docking indicated that ferulic acid was a proper inhibitor for the 4HI0 protein enzyme of H. pylori. A low energy score (−5.58 Kcal/mol) was recorded as a result of the interaction of ferulic acid with the residue’s SER 139 active site caused by the O 29 atom, which was important for its antibacterial activity.

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Section: Antioxidant Activity a Nilotica Flower Extractmentioning

confidence: 99%

Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico

Al-Rajhi

Qanash

Bazaid

et al. 2023

JFB

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“…The plates were incubated for 3 days at 30°C and sensitivity of the mutant strains towards increasing concentration of H 2 O 2 was determined. YPDA plates devoid of H 2 O 2 served as control (Subhaswaraj et al 2017).…”

Section: Antioxidant Activitymentioning

confidence: 99%

Evaluation of in vivo antioxidant potential of Syzygium jambos (L.) Alston and Terminalia citrina Roxb. towards oxidative stress response in Saccharomyces cerevisiae

2018

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Excessive production and restricted elimination of free radicals like superoxide, hydroxyl radical (Á OH), anion radical (O 2 Á-), and non-radical hydrogen peroxide (H 2 O 2) are related to the development of cancer, arteriosclerosis, arthritis and neurodegenerative diseases. According to a report of World Health Organisation, about 80% of the population living in the developing countries predominantly depends on the traditional medicine for their primary healthcare. Plants possess innate ability to synthesize a wide variety of enzymatic and non-enzymatic antioxidants capable of attenuating ROS-induced oxidative damage. The ethanolic leaf extracts of Syzygium jambos L. and Terminalia citrina Roxb. exhibited a significant in vitro antioxidant activity when compared with natural antioxidant, ascorbic acid. The extracts also provided strong cellular protection against the damaging effects of H 2 O 2 induced oxidative stress in the mutant strains (tsa1D and sod1D) of Saccharomyces cerevisiae. The GC-MS analysis of the leaf extracts revealed the presence of phytoconstituents majorly constituting of terpenes, vitamin and fatty acids contributing to the antioxidant property. The plant extracts may serve as a potential source of exogenous antioxidants to combat the undesirable effects of oxidative stress.

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“…The authors of the article “Determination of antioxidant potential of Acacia nilotica leaf extract in oxidative stress response system of Saccharomyces cerevisiae ” (DOI: 10.1002/jsfa.8409), published in Wiley Online Library on 05 May 2017 and in the Journal of the Science of Food and Agriculture , 97: 5247–5253, have drawn our attention to some errors in Figure 5, where the panels C, D and F, G were self‐replicated and panels E and H were erroneously represented. The corrected figure is showed below.…”

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Determination of antioxidant potential of Acacia nilotica leaf extract in oxidative stress response system of Saccharomyces cerevisiae

et al. 2018

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Determination of antioxidant potential ofAcacia niloticaleaf extract in oxidative stress response system ofSaccharomyces cerevisiae

Abstract: The results suggested the efficacy of A. nilotica as a potent antioxidant in the S. cerevisiae system for the first time and its use in neutraceuticals/therapeutics. © 2017 Society of Chemical Industry.

Cited by 14 publications

References 39 publications

Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico

Pharmacological Evaluation of Acacia nilotica Flower Extract against Helicobacter pylori and Human Hepatocellular Carcinoma In Vitro and In Silico

Evaluation of in vivo antioxidant potential of Syzygium jambos (L.) Alston and Terminalia citrina Roxb. towards oxidative stress response in Saccharomyces cerevisiae

Determination of antioxidant potential of Acacia nilotica leaf extract in oxidative stress response system of Saccharomyces cerevisiae

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