Determination of Antioxidant, Cytotoxicity, and Acetylcholinesterase Inhibitory Activities of Alkaloids Isolated from Sophora flavescens Ait. Grown in Dak Nong, Vietnam

Abstract

Traditional/herbal medicine has gained increasing interests recently, especially in Asian countries such as Vietnam, due to its diverse therapeutic actions. In the treasure of Vietnamese medicinal plants, one of the potential herbs is the roots of Sophora flavescens Ait. (SF, “Kho sam” in Vietnamese). However, limited information has been reported on the Vietnamese SF compositions and their respective alkaloids’ anti-acetylcholinesterase action. Thus, this study investigated the extractions, isolations, identifications, and in-vitro antioxidant, cytotoxicity, and acetylcholinesterase inhibitory activities, of the SF root extracts and their purified alkaloid compounds. To this end, four pure compounds were successfully isolated, purity-tested by HPLC, and structurally identified by spectroscopic techniques of FTIR, MS, and NMR. These compounds, confirmed to be oxysophocarpine, oxymatrine, matrine, and sophoridine, were then determined their therapeutic actions. The SF extracts and the compounds did not possess significant antioxidant activity using the DPPH and MDA assays, and cytotoxicity action using the MTT assay on the MDA-MB-231 breast cancer cell line. On the other hand, the SF total extract yielded a moderate acetylcholinesterase inhibition effect, with an IC50 of 0.1077 ± 0.0023 mg/mL. In summary, the SF extract demonstrated potential effects as an anti-acetylcholinesterase agent and could be further researched to become a pharmaceutical product for diseases related to acetylcholine deficiency, such as dementia.

Protective effect of five-flavor sophora flavescens enteric-coated capsules on inflammatory bowel disease and its molecular mechanism

“…Raw licorice is rich in glycyrrhizic acid, glycyrrhizin and other active components, which have antiviral, antioxidant and other pharmacological effects (Song et al, 2022). Although FSEC is a new national drug developed for UC, domestic and foreign scholars have conducted a large number of studies on the chemical composition and pharmacological effects of monodrug in this Chinese patent medicine (Thang et al, 2022;, and they have achieved certain results, the compound mechanism of action in each component is still unclear. However, there is still a lack of in-depth understanding and research on its mechanism.…”

Biochar and organic fertilizer drive the bacterial community to improve the productivity and quality of Sophora tonkinensis in cadmium-contaminated soil

“…Soil enzyme activity was measured using the method by İbrahim and Md ( İbrahim et al, 2020 ; Md et al, 2020 ). The Cd content of plants was determined by inductively coupled plasma mass spectrometry, while matrine and oxymatrine content was measured by the HPLC method ( Thang et al, 2022 ).…”

Extractions, Standardizations, and In-Vivo Toxicological Investigations of The Vietnamese Fish Mint (Houttuynia cordata Thunb.)

“…The plant physicochemical constants of humidity, foreign matter, total ash, crumbling rate, extraction efficiency, and essential oils quantitation were carried out on based on the Vietnamese Pharmacopoeia V. Briefly, the plant humidity (water content) should be <13% and was determined by distillation method. The percentage of water in the test sample was calculated according to equation (1), with V1 (mL) and V2 (mL) is the volume of water in the receiver after the first and second distillation, respectively, and m is the sample weight.…”

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IntroductionRecently, research on herbal/traditional medicine and their relevant products have gained increasingly interests in numerous biomedical areas. [1][2][3][4][5][6][7][8] One of the potential herbal plant is Houttuynia cordata Thunb. (HC, fish mint), a perennial herb with heart-like leaf and stoloniferous rhizome native to Japan, Southeast Asia, and the Himalayas.

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