Determination of Antioxidant Activity for Metal Ions Complexes

Jirjees, Vian Yamin; Suleman, Veyan Taher; Ahmed, Suzan; Al‐Hamdani, Abbas Ali Salih

doi:10.26682/sjuod.2020.23.1.5

Cited by 4 publications

(4 citation statements)

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“…Similarly, HeLa cell SC 50 's were 361 ± 1.2, 619.5 ± 0.86, and 835.5 ± 0.63 μL/mL, respectively, compared to 814.5 ± 1.45 μL/mL in untreated cells. Better DPPH radical scavenging efficacy is indicated by lower SC 50 values [ 132 ]. Complex one in MCF-7 cells exhibits higher scavenging activity than other complexes and control cells.…”

Section: Resultsmentioning

confidence: 99%

Study of novel bidentate heterocyclic amine-based metal complexes and their biological activities: cytotoxicity and antimicrobial activity evaluation

et al. 2023

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Metallic antitumor drugs with heterocyclic ligands, such as novel AMI (amino methyl imidazole) complexes [Pd(AMI)Cl2](1), [Cu(AMI)L1](2), and [Cu(AMI)L2·2H2O](3) where L1 = oxalate and L2 = malonate, were synthesized and characterized. Assessments included elemental analyses, mass spectrometry, Fourier transform-infrared spectroscopy, ultraviolet–visible spectroscopy, and thermal analysis. The cytotoxicity of AMI complexes compared to cisplatin was assessed using MTT (3-[4,5-dimethylthiazol-2-yl] 2,5diphenyl tetrazolium bromide) assay with breast (MCF-7) and cervical (HeLa) cancer cell lines. After treating these cells with the AMI complexes' IC50 values for 48 h, malondialdehyde levels and catalase activity were used to assess oxidative stress, antioxidant activity was evaluated with DPPH radical scavenging method, comet assays assessed DNA damage, and DNA fragmentation was evaluated using the gel electrophoresis. In vitro, antimicrobial activity was assessed using a disc diffusion method. The anticancer activity results showed that IC50 (half-maximal inhibitory concentration) values of complex one, two, and three against MCF-7 and HeLa cancer cells are 0.156 ± 0.0006, 0.125 ± 0.001, 0.277 ± 0.002 μM respectively for MCF-7 cells and 0.222 ± 0.0005, 0.126 ± 0.0009, 0.152 ± 0.001 μM respectively for HeLa cells. Complex two demonstrated strong anticancer activity against MCF-7 and Hela cells. The study of oxidative stress parameters revealed that Malondialdehyde levels increased in cancer cell lines treated with complexes compared to untreated cells. Catalase activity decreased in cells treated with palladium chelate. The DPPH radical scavenging assay results identified that complex one was a more potent antioxidant in MCF-7 and Hela cells than other complexes with SC50 values of 227.5 ± 0.28 and 361 ± 1.2 μL/mL, respectively. The comet assay results showed that complex two caused significant DNA damage in MCF-7 and HeLa cancer cells treated. Antimicrobial assays identified complex three as the most effective. Copper complexes give better antifungal activity against A. flavus than the palladium complex. We conclude that complex two is the most active in both cell types and might be assessed as a clinically useful drug for breast cancer treatment. The significance of the current study is the synthesis of antitumor drugs containing heterocyclic ligands, such as novel AMI complexes, and the study of their biological activities.

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Section: Resultsmentioning

confidence: 99%

Study of novel bidentate heterocyclic amine-based metal complexes and their biological activities: cytotoxicity and antimicrobial activity evaluation

et al. 2023

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“…DPPH (2,2‐diphenyl‐1‐picryl‐hydrazyl‐hydrate) is a stable chromogenic radical used in assays to measure the antioxidant activity of molecules, governed by a HAT (hydrogen atom transfer) and SET (electron transfer) mechanism, which can be followed by spectrophotometry at 517 nm [45–47] . DMSO was the reference, while BHT was the positive control due to its well‐known antioxidant activity [48–51] . Table 2 presents the percentage of reduction of samples ( 1 )–( 6 ) and the reference BHT calculated according to Equation 2 in materials and methods.…”

Section: Resultsmentioning

confidence: 99%

“…[45][46][47] DMSO was the reference, while BHT was the positive control due to its wellknown antioxidant activity. [48][49][50][51] Table 2 presents the percentage of reduction of samples (1)-( 6) and the reference BHT calculated according to Equation 2 in materials and methods.…”

Section: Antioxidant Activity Of Copper Complexesmentioning

confidence: 99%

Antileishmanial and Anti‐Chikungunya Activity of Cu(I)‐N‐Heterocyclic Carbenes

et al. 2022

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Neglected tropical diseases such as Leishmaniasis and Chikungunya fever are worldwide public health challenges mainly affecting tropical and subtropical countries. One cationic [Cu-(I)(NHC) 2 ] + (4) and two neutral [Cu(I)(NHC)Cl] complexes, where NHC = 1,3-bis(mesityl)imidazole-2-ylidene (IMes) (5) or 1,3-bis-(2,6-diisopropylphenyl)imidazole-2-ylidene (IPr) (6), had their in vitro activity evaluated towards Leishmania amazonensis and Chikungunya virus (CHIKV). The compound (6) inhibited 95 % of L. amazonensis infection in RAW macrophages at 10 μM and 90 % of the CHIKV replication at 2 μM in BHK-21 cells. Otherwise, the other compounds showed higher cytotoxicity in BHK-21 and RAW or lower antiparasitic or antiviral activities.The best activity of compound 6 can be explained by slower ligand exchange with solvent molecules measured by 1 H NMR technique and the best hydrophilic/lipophilic balance (log P = À 0.684 � 0.055) in the series determined by shake flask method. Antioxidant activity measured by reduction of DPPH revealed a moderate redox ability of all complexes. The binding constant of ( 6) with bovine serum albumin (BSA) represents the weakest in the series (10 3 ). The chemical and in vitro evaluation reported here represent a novel application and chemical insights for the design of [Cu(I)(NHC)L] metallodrugs for these infectious diseases.

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“…There are many palladium Schiff base complexes synthesized for this purpose in the literature. It was stated that these complexes generally exhibited average antioxidant activity and antioxidant property of the complexes were greater than Schiff bases [23][24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Palladium complexes of NO type Schiff bases: synthesis, characterization and antioxidant activities

Şenocak

Akbaş

2021

Cumhuriyet Science Journal

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The reaction of o-aminophenol with para hydroxy and methoxy substituted benzaldehydes yielded two Schiff bases. These Schiff bases functioning precursors were turned into palladium complexes by treating palladium acetate. The structural formulas based on spectral methods (elemental analysis, FT-IR spectroscopy, NMR spectroscopy and QTOF-LC/MS spectroscopy) were suggested for obtained both complexes. According to the structural characterization methods, one of the complexes had also an acetate co-ligand along with the hydroxy substituted Schiff base ligand. In the other complex, the palladium central ion was proposed to be coordinated with two methoxy substituted Schiff bases. Besides, DPPH scavenging activities of the all synthesized compounds were determined and compared to well-known antioxidant standards. According to the results, antioxidant activities of the palladium complexes was mild but lower than parent Schiff bases.

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Determination of Antioxidant Activity for Metal Ions Complexes

Cited by 4 publications

References 0 publications

Study of novel bidentate heterocyclic amine-based metal complexes and their biological activities: cytotoxicity and antimicrobial activity evaluation

Study of novel bidentate heterocyclic amine-based metal complexes and their biological activities: cytotoxicity and antimicrobial activity evaluation

Antileishmanial and Anti‐Chikungunya Activity of Cu(I)‐N‐Heterocyclic Carbenes

Palladium complexes of NO type Schiff bases: synthesis, characterization and antioxidant activities

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