Determination of antimigraine compounds rizatriptan, zolmitriptan, naratriptan and sumatriptan in human serum by liquid chromatography/electrospray tandem mass spectrometry

Vishwanathan, Karthick; Bartlett, Michael G.; Stewart, James T.

doi:10.1002/(sici)1097-0231(20000215)14:3<168::aid-rcm861>3.0.co;2-9

Cited by 72 publications

(49 citation statements)

References 22 publications

(19 reference statements)

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“…Literature survey reveals many methods for estimation of SUM [4][5][6][7][8][9][10][11][12] and NAP [13][14][15][16][17] individually and very few methods are available for simultaneous determination by UV [18][19][20] , HPTLC 21 and HPLC [22][23][24][25][26] .…”

Section: Introduction: Sumatriptanmentioning

confidence: 99%

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2012

IJPSR

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This investigation describes a new precise, sensitive and accurate RP-HPLC method for the Simultaneous estimation of Sumatriptan succinate and Naproxen Sodium in Bulk and Tablets. The resolution of two drugs was achieved on XTerra C 18 (150mm x 4.6mm i.d., 3.5m particle size) column with UV detection at 284nm and the mobile phase consists of Buffer and Acetonitrile (25:75 v/v). Using chromatographic conditions described Sumatriptan succinate and Naproxen Sodium were well resolved with mean retention times of 2.622 and 4.070 min, respectively. Linear response (r>0.999) was observed over the range of 30-70µg/ml for Sumatriptan succinate and 20-60µg/ml for Naproxen Sodium. The lower limit of quantification and lower limit of detection was 10.02 and 3.0 for Sumatriptan succinate and 10.05 and 2.99 for Naproxen Sodium. The Validation parameters were performed according to the ICH guidelines and the proposed method can be useful in the routine analysis for the determination of Sumatriptan succinate and Naproxen Sodium in Pharmaceutical dosage forms.

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Section: Introduction: Sumatriptanmentioning

confidence: 99%

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2012

IJPSR

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“…So far, several liquid chromatography procedures have been described for the determination of SS and NS [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. But, these procedures were developed to estimate either SS or NS individually Page 2 of 6 and in combination with other drugs from formulation, plasma, urine, intestinal perfusion samples and in bulk drugs.…”

Section: Introductionmentioning

confidence: 99%

Rapid Simultaneous Determination of Sumatriptan Succinate and Naproxen Sodium in Combined Tablets by Validated Ultra Performance Liquid Chromatographic Method

Reddy¹,

Kumar²,

Reddy³

et al. 2011

J Anal Bioanal Techniques

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A stability-indicating ultra perfomance liquid chromatography (UPLC) method was developed for the simultaneous determination of sumatriptan succinate and Naproxen sodium in pharmaceutical dosage forms. The chromatographic separation was achieved on C18, 50 mm × 4.8 mm, 1.8-µm particle size column. The mobile phase contains a mixture of 0.2% ortho phosphoric acid and acetonitrile as the mobile phase in gradient elution technique. The retention time of Sumatriptan and Naproxen was found to be 1.7 and 2.7 min respectively. The total runtime was 5 min within which two active compounds and degradation products were separated. This method allows the determination of 850-2565 µg mL -1 of sumatriptan succinate and 5000-15000 µg mL -1 of Naproxen sodium. The flow rate was 1.0 mL min -1 and the detection wavelength was 225 nm. The limit of detection (LOD) for sumatriptan succinate and Naproxen sodium was 1.9 and 1.5 µg mL -1 , respectively. The limit of quantification (LOQ) for sumatriptan succinate and Naproxen sodium was 6.3 and 4.8 µg mL -1 , respectively. This method was validated for accuracy, precision, linearity and robustness. sumatriptan succinate and Naproxen sodium were subjected to different ICH prescribed stress conditions of oxidative, acid, base, hydrolytic, thermal and photolytic degradation and the method was also found to be stability indicating. Ultra performance liquid chromatography (UPLC) is a recent technique in liquid chromatography, which enables significant reductions in separation time and solvent consumption. Literature indicates that UPLC system allows about ninefold decrease in analysis time as compared to the conventional HPLC system using 5μm particle size analytical columns, and about threefold decrease in analysis time in comparison with 3μm particle size analytical columns without compromise on overall separation [23][24][25][26][27]. Rapid Simultaneous Determination of SumatriptanHence, a rapid, accurate stability indicating UPLC method for the simultaneous determination of SS and NS from combined dosage form was developed and validated. Experimental Instrumentation and chromatographic conditionsThe Waters UPLC Acquity system we used consists of a binary solvent manager, a sample manager and a UV detector. Zorbax SB C-18, 50 mm x 4.6 mm i.d with 1.8µm particles was used as stationary phase. 0.2% ortho phosphoric acid in water (pH 2.2) as solvent A and acetonitrile and water in the ratio 90:10; v/v, was as solvent B used for mobile phase. Prior to use, the mobile phase was mixed thoroughly and degassed. ReagentsStandards were supplied by D.C.O. Hyderabad, India. HPLC grade acetonitrile, analytical grade ortho phosphoric acids were purchased from Merck (Mumbai, India). Water was prepared by Millipore MilliQ Plus water purification system. Commercial pharmaceutical preparation of Triximet combined tablets were purchased from the market. The declared content of tablets was Sumatriptan 85 mg and Naproxen 500 mg per tablet. Preparation of solutionsStandard solutions: A standard so...

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“…human plasma [9][10][11][12][13][14][15][16][17], dog plasma [18] and rat plasma [19], and in pharmaceutical formulations [20][21][22]. These methods include LC-MS [9-12, 18, 20] and high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection [13-17, 19, 21].…”

Section: Introductionmentioning

confidence: 99%

“…These methods include LC-MS [9-12, 18, 20] and high-performance liquid chromatography (HPLC) with ultraviolet (UV) detection [13-17, 19, 21]. Among these, the best quantification results achieved for RZ in human plasma were by the LC-MS/MS method [10,11,12].…”

Section: Introductionmentioning

confidence: 99%

Determination of Rizatriptan in Human Plasma by Liquid Chromatography Stable Isotope Dilution Electrospray MS–MS for Application in Bioequivalence Study

et al. 2011

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A simple, sensitive, selective, rapid, rugged, reproducible and specific liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was used for quantitative estimation of rizatriptan (RZ) in human plasma using rizatriptan-d 6 (RZD6) as internal standard (IS). Chromatographic separation was performed on Ascentis Express RP Amide C18, 50 9 4.6 mm, 2.7 lm column with isocratic mobile phase composed of 10 mM ammonium formate:acetonitrile (20:80 v/v) at flow rate of 0.5 mL min -1 . RZ and RZD6 were detected with proton adducts at m/z (amu) 270.2 ? 201.2 and 276.1 ? 207.1, respectively, in multiple reaction monitoring (MRM) positive mode. Liquid-liquid extraction was used and validated over a linear concentration range of 0.1-100.0 ng mL -1 with correlation coefficient r 2 C 0.9981. The limit of quantification (LOQ) and limit of detection (LOD) were found to be 0.1 ng mL -1 and 12.5 fg, respectively. Intra-and inter-day precision were within 1.7-3.1% and 2.8-3.7%, and accuracy within 96.0-101.7% and 99.7-101.4% for RZ. Drug was found to be stable throughout three freeze-thaw cycles. The method was successfully employed for analysis of plasma samples following oral administration of RZ (10 mg) in 25 healthy Indian male human volunteers under fasting conditions.

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Determination of antimigraine compounds rizatriptan, zolmitriptan, naratriptan and sumatriptan in human serum by liquid chromatography/electrospray tandem mass spectrometry

Cited by 72 publications

References 22 publications

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Rapid Simultaneous Determination of Sumatriptan Succinate and Naproxen Sodium in Combined Tablets by Validated Ultra Performance Liquid Chromatographic Method

Determination of Rizatriptan in Human Plasma by Liquid Chromatography Stable Isotope Dilution Electrospray MS–MS for Application in Bioequivalence Study

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