Determination of antiepileptic drugs

A simple and rapid determination of valproic acid in human plasma using a non‐porous silica column and liquid chromatography with tandem mass spectrometric detection

“…In the accuracy test, the recovery of VPA was more than 82.3% as determined by the addition of a certain amount of VPA to human blank plasma. LOQ obtained from the present methods was 0.5 μg/mL, which was comparable to those reported elsewhere (Kushida and Ishizaki, 1985;Lingeman and Underberg, 1990;Amini et al, 2006;Lin et al, 2004;Burke and Thenot, 1985;Yu et al, 1995;Mino et al, 2001). The LOD was 0.17 μg/mL, a concentration that was lower than the therapeutic concentrations (50 -100 μg/mL).…”

“…The LOD was 0.17 μg/mL, a concentration that was lower than the therapeutic concentrations (50 -100 μg/mL). The sensitivity of the our method was comparable to those reported by other methods (Kushida and Ishizaki, 1985;Lingeman and Underberg, 1990;Amini et al, 2006;Lin et al, 2004;Burke and Thenot, 1985;Yu et al, 1995;Mino et al, 2001;Ramakrishna et al, 2005). However, our LOD (0.17 μg/mL) for VPA did not show the best performance as compared with the LOD (0.075 μg/mL) reported by Pucci et al (2005).…”

“…Several methods for the assay of VPA, including immunoassay (Ehrenthal and Rochel, 1982), highperformance liquid chromatographic (HPLC) assay with ultraviolet detection (Kushida and Ishizaki, 1985;Lingeman and Underberg, 1990;Amini et al, 2006), fluorescence detection (Lin et al, 2004) or gas chromatography with mass spectrometric analysis (Burke and Thenot, 1985;Yu et al, 1995), have been reported to detect therapeutic concentrations of VPA in plasma. However, most of these methods are time-consuming due to the complicated procedures for sample preparation, derivatization and extraction.…”

Development and validation of a sensitive assay of valproic acid in human plasma by high-performance liquid chromatography without prior derivatization

“…Due to the widespread usage of valproic acid as an antiepileptic drug, there is a constant need for simple and specific methods for the determination of this substance in patients' plasma. Since valproic acid is volatile, it is mostly determined by gas chromatography (GC) without derivatization or following alkylation [2]. High-performance liquid chromatography (HPLC) is an attractive alternative to GC for the analysis of most drug, however, its application for the analysis of valproic acid in plasma is limited by the fact that the drug has no suitable chromophore.…”

Detection of anticonvulsants and their metabolites in urine within a “general unknown” analysis procedure using computerized gas chromatography-mass spectrometry

“…The identified drugs can then be quantified in plasma using methods described by Juergens (1986), Pang et al (1986), Mira et al (1987), Matsumoto et al (1988), Richard et al (1989) and Svinarov and Dotchev (1989) or reviewed by Burke and Thrnot (1985). Finally, several immunoassay techniques are commercially available for therapeutic drug monitoring of anticonvulsants.…”