Determination of antidepressants in whole blood using hollow-fiber liquid-phase microextraction and gas chromatography–mass spectrometry

Santos, Marcelo Filonzi dos; Ferri, Caio Caleiras; Seulin, Saskia Carolina; Leyton, Vilma; Pasqualucci, Carlos Augusto; Muñoz, Daniel Romero; Yonamine, Maurı́cio

doi:10.1007/s11419-014-0226-9

Cited by 23 publications

(6 citation statements)

References 29 publications

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“…The linearity range was assessed between 10.0 and 1000.0 µg L −1 (nine concentration levels). This range was chosen once it included the therapeutic, toxic and lethal concentration values for TCAs [ 45 ]. For the calibration plots, we started by plotting a conventional linear regression, which showed good linearity ( r 2 ≥ 0.9914), and according to the lack-of-fit test (at the confidence level 95%), it presented a good fit for the present study, i.e., the F calc was always below the F tab .…”

Section: Resultsmentioning

confidence: 99%

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Application of Bar Adsorptive Microextraction for the Determination of Levels of Tricyclic Antidepressants in Urine Samples

et al. 2021

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This work entailed the development, optimization, validation, and application of a novel analytical approach, using the bar adsorptive microextraction technique (BAμE), for the determination of the six most common tricyclic antidepressants (TCAs; amitriptyline, mianserin, trimipramine, imipramine, mirtazapine and dosulepin) in urine matrices. To achieve this goal, we employed, for the first time, new generation microextraction devices coated with convenient sorbent phases, polymers and novel activated carbons prepared from biomaterial waste, in combination with large-volume-injection gas chromatography-mass spectrometry operating in selected-ion monitoring mode (LVI-GC-MS(SIM)). Preliminary assays on sorbent coatings, showed that the polymeric phases present a much more effective performance, as the tested biosorbents exhibited low efficiency for application in microextraction techniques. By using BAμE coated with C18 polymer, under optimized experimental conditions, the detection limits achieved for the six TCAs ranged from 0.2 to 1.6 μg L−1 and, weighted linear regressions resulted in remarkable linearity (r2 > 0.9960) between 10.0 and 1000.0 μg L−1. The developed analytical methodology (BAμE(C18)/LVI-GC-MS(SIM)) provided suitable matrix effects (90.2–112.9%, RSD ≤ 13.9%), high recovery yields (92.3–111.5%, RSD ≤ 12.3%) and a remarkable overall process efficiency (ranging from 84.9% to 124.3%, RSD ≤ 13.9%). The developed and validated methodology was successfully applied for screening the six TCAs in real urine matrices. The proposed analytical methodology proved to be an eco-user-friendly approach to monitor trace levels of TCAs in complex urine matrices and an outstanding analytical alternative in comparison with other microextraction-based techniques.

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Section: Resultsmentioning

confidence: 99%

“…The data achieved shows that a weighting factor of 1/y resulted in the lowest %RE across the whole range. The weighting factor should be used in the calculation of the regression equation parameters as described in previous works [ 45 , 46 , 47 , 48 ].…”

Section: Resultsmentioning

confidence: 99%

Application of Bar Adsorptive Microextraction for the Determination of Levels of Tricyclic Antidepressants in Urine Samples

et al. 2021

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“…Helium was used as carrier gas at a flow rate of 0.8 mL/min while splitless injection mode was chosen. LOQs were 20 ng/mL and recoveries ranged 36-89% [67].…”

Section: Gas Chromatography-mass Spectrometry Methodsmentioning

confidence: 99%

Applications of Gas Chromatography for the Analysis of Tricyclic Antidepressants in Biological Matrices

Manousi

Samanidou

2019

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Tricyclic antidepressant drugs (TCAs) are a main category of antidepressants, which are until today widely used for the treatment of psychological disorders due to their low cost and their high efficiency. Therefore, there is a great demand for method development for the determination of TCAs in biofluids, especially for therapeutic drug monitoring. Gas chromatography (GC) was the first chromatographic technique implemented for this purpose. With the recent development in the field of sample preparation, many novel GC applications have been developed. Herein, we aim to report the recent application of GC for the determination of tricyclic antidepressants in biofluids. Emphasis is given to novel extraction techniques and novel materials used for sample preparation.

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“…= not reported; Na2SO4 = sodium sulfate; NaCl = sodium chloride; NDMD = N-desmethyldiazepam; NPD = nitrogen-phosphorus detector; OF = oral fluid; P = plasma; PDHID = pulsed-discharge helium ionization detector-helium ionization detector; PP = polypropylene; ps = pore size; PVDF = polyvinylidene difluoride; T = temperature; THC-COOH = 11-nor-Δ9-tetrahydrocannabinol-9-carboxylic acid; THC = Δ9-tetrahydrocannabinol; wt = wall thickness; U = urine; ULC = ultrahigh performance liquid chromatography; UV = ultra-violet 2.5. Three-phase HF-LPME One hundred and forty-two bioanalytical methods were described in 136 publications fit the inclusion criteria (Table 3) [64][65][66][67][68][69][70][71], breast milk [72][73][74], serum [75,76], oral fluid [77,78], hair [79], or liver [80].…”

Section: Methylbenzolmentioning

confidence: 99%

A review of the application of hollow-fiber liquid-phase microextraction in bioanalytical methods – A systematic approach with focus on forensic toxicology

Venson

Korb

Cooper

2019

Journal of Chromatography B

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Over the past three decades, many studies employing hollow-fiber liquid-phase microextraction (HF-LPME) bioanalytical methods have been published. The basic mechanism of extraction relies on the migration of the analytes through a liquid membrane sustained in the pores of the walls of a porous hollow fiber, and from there into an acceptor phase present in the lumen of the fiber. The mass transfer occurs by passive diffusion and it can be enhanced by using a carrier or applying an electrical potential across the phases. This type of extraction method presents many advantages over classical techniques, such as high preconcentration factor, clean extracts, and a green chemistry approach. Due to its advantages, and considering that no study systematically compiled the characteristics of the published methods in one single accessible source of information, the aim of this systematic review is to assess the data regarding bioanalytical methods, compile, and analyse the studies published until up to October of 2017. The data source used for the systematic review were Pubmed, Web of Science, and Science Direct, and 171 studies were included in the final review by two independent reviewers, resulting in a reliable and accessible source of information about bioanalytical methods employing HF-LPME.

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Determination of antidepressants in whole blood using hollow-fiber liquid-phase microextraction and gas chromatography–mass spectrometry

Cited by 23 publications

References 29 publications

Application of Bar Adsorptive Microextraction for the Determination of Levels of Tricyclic Antidepressants in Urine Samples

Application of Bar Adsorptive Microextraction for the Determination of Levels of Tricyclic Antidepressants in Urine Samples

Applications of Gas Chromatography for the Analysis of Tricyclic Antidepressants in Biological Matrices

A review of the application of hollow-fiber liquid-phase microextraction in bioanalytical methods – A systematic approach with focus on forensic toxicology

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