Determination of anticholinesterase pesticides in real samples using a disposable biosensor

Palchetti, Ilaria; Cagnini, A.; Carlo, Michele Del; Coppi, C.; Mascini, Marco; Turner, Anthony P. F.

doi:10.1016/s0003-2670(96)00418-7

Cited by 194 publications

(94 citation statements)

References 13 publications

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“…The few reports of cholinesterase-based biosensors for food testing either relied on laborious multi-step sample preparation or showed problems due to matrix effects reducing the accuracy of the results [17][18][19][20][21]. Furthermore, these tests were limited to carbamates and oxoforms of organophosphates although most of the found organophosphorus insecticides are sulfur-containing compounds, especially those with a P=S moiety (e.g.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction

Schulze

Schmid

Bachmann

2002

Analytical and Bioanalytical Chemistry

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The extensive use of pesticides to protect agricultural crops necessitates reliable tools for the detection of residues in food and water, thus ensuring environmental protection and consumer safety. Neuroinhibitors such as organophosphates and carbamates in particular, represent a potential hazard to human health. These compounds are frequently found in food but conventional methods of analysis are limited as they are either time consuming or not sufficiently sensitive. As a result, a rapid and sensitive biosensor test based on AChE-inhibition was developed. The disposable AChE-biosensor was directly applied in solvent extracts of food samples using isooctane as extraction solvent. A complete assay could be performed in less than 2 hours. Recovery rates of 84 % were obtained in tests with spiked orange juice samples. Tests in food samples with a lower water content resulted in reduced recovery rates (44 % for peach pap baby food). Phosphorothionate insecticides could be detected after direct oxidation in food with N-bromosuccinimide and solvent extraction. The assay displayed a detection limit of 2 µg/kg paraoxon which was sufficient for the monitoring of maximum residue limits in food according to EU regulations.3

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Section: Introductionmentioning

confidence: 99%

Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction

Schulze

Schmid

Bachmann

2002

Analytical and Bioanalytical Chemistry

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“…São sensores do tipo impressos ou serigrafados, onde as camadas sub-seqüenciais eletródicas são depositadas sobre suportes finos, como cerâmica e plásticos, sendo que a enzima pode ser imobilizada sobre o eletrodo, em pequenas quantidades, por variadas metodologias, possibilitando tanto análises rápidas e baratas, quanto a aplicação in situ. [31][32][33][34][35][36] …”

Section: Materiais Empregadosunclassified

“…Eles demonstraram que a atividade anticolinesterase apresentada pelo biossensor foi menor para o sistema binário, que para o somatório das atividades anticolinesterases individuais, indicando que uma mesma gama de amostras reais pode fornecer respostas distintas quando contaminada por mais de um pesticida. Palchetti et al 36 estudaram a atividade anticolinesterase de pesticidas organofosforados e carbamatos em soluções padrões e em amostras reais, avaliando o efeito de solventes orgânicos no meio. As curvas de calibração apresentaram comportamentos diferentes, quando distintos solventes orgânicos estavam presentes no meio, sendo que o acetato de etila foi o que mais afetou a atividade enzimática, evidenciando efeitos de inibição deste solvente sobre o biossensor.…”

Section: (Reação 7)unclassified

“…Áreas como a saúde e militar são potenciais de aplicação destes biossensores. 36,41,[59][60][61] Hart, Collier e Janssen, 62 avaliando a resposta de biossensores baseados em colinesterases frente a formulações comerciais contendo organofosforados, indicaram que o sistema pode ser uma plataforma viável para aplicações em indústrias, nas áreas de controle de qualidade. Kumaran e Morita 59 aplicaram um biossensor construído com enzima butirilcolinesterase imobilizada em membrana, acoplada a eletrodo de pH, na detecção de organofosforados extraídos de amostras de solos, demonstrando a potencialidade deste sistema para aplicação em análise desta complexa matriz.…”

Section: (Reação 8)unclassified

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Biossensores baseados no processo de inibição enzimática

Marques¹,

Yamanaka²

2008

Quím. Nova

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Recebido em 3/5/07; aceito em 30/11/07; publicado na web em 4/9/08 BIOSENSORS BASED ON THE ENZYMATIC INHIBITION PROCESS. Fast, selective, reproducible and reliable detections have been carried out by using enzymatic biosensors in several areas. The enzymatic biosensors based on the inhibition represent an important role in analytical chemistry. Enzymes like cholinesterases, peroxidases, tyrosinases, etc. have been immobilized on electrochemical and optical transducers and the enzymatic activity decreasing in the presence of the inhibitor is related with its concentrations. This article presents a review on the enzymes used on the construction of these sensors, emphasizing the respective applications.

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“…This can be carried out using enzymes [9], acetyl cholinesterase (AChE) and organophosphate hydrolase (OPH) modified amperometric [10]. AChE inhibition-based biosensors are sensitive and useful as single-use disposable sensors for environmental monitoring.…”

mentioning

confidence: 99%

Metal Nano Layer Coating for Improving the Detection and Recognition of Micro-Poisons Using Reflection Spectroscopic Measurement

Abramovich¹,

Shulzinger²,

Ochana³

et al. 2015

OPJ

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Metal nano layer coating for increasing the sensitivity of spectroscopic measurements is proposed and experimentally demonstrated in this paper. The metal nano layer will attract the micropoisons from any measured aqueous sample increasing the concentration of the micro-poison in the vicinity of the surface and significantly improves the sensitivity of the spectroscopic measurement. The demonstration was carried out using Fourier Transform Infra-Red (FTIR) operating in the MIR 400 cm −1 -4000 cm −1 and 5 nm Gold layer which was grown on silicon oxide substrate. In the experimental demonstration Malathion organophosphate pesticide was used as micro-poison. The spectroscopic measurement proves that Malathion was attracted to the metal nano layer. Furthermore, the absorption lines of Malathion were detected and recognized. This proof of principle can be applied to any Internal Reflection Elements (IRE) and it can be used to purify any aqueous solutions and atmosphere from micro-poisons which will be attracted to the metal Nano layer.

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Determination of anticholinesterase pesticides in real samples using a disposable biosensor

Cited by 194 publications

References 13 publications

Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction

Rapid detection of neurotoxic insecticides in food using disposable acetylcholinesterase-biosensors and simple solvent extraction

Biossensores baseados no processo de inibição enzimática

Metal Nano Layer Coating for Improving the Detection and Recognition of Micro-Poisons Using Reflection Spectroscopic Measurement

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