Determination of antibodies to pertussis toxin in working reference preparations of anti-pertussis sera from various national control laboratories

Relyveld, E. H.; Oato, Noriko Hikino; Huet, M.; Gupta, Rajesh K.

doi:10.1016/s1045-1056(05)80009-0

Cited by 7 publications

(1 citation statement)

References 11 publications

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“…In short, ELISA was applied using purified PT (JNIH-5, Biken, Japan), Fc-specific alkaline phosphatase-conjugated goat anti-human IgG (Seikagaku Kogyo, Tokyo, Japan), and p-nitrophenyl phosphate (Wako, Tokyo, Japan). Anti-PT IgG antibody concentrations were calculated by the parallel line assay and expressed in ELISA unit (EU)/mL according to the reference anti-sera for PT (JNIH-10; 250 EU/ampule for anti-PT) (17). In addition, a sera with a known antibody titer was included in each test for quality control.…”

Section: Measurement Of Immunoglobulin G (Igg) Anti-pt Antibody By Inmentioning

confidence: 99%

Prevalence of high antibody titers of pertussis in Turkey: reflection of circulating microorganism and a threat to infants

Esen¹,

Çöplü²,

Kurtoğlu³

et al. 2007

Clinical Laboratory Analysis

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Acute pertussis infection among adults can cause its transmission to the larger population, especially to infants and young children, who can develop severe disease. In order to determine an age-dependent pertussis immune response, anti-pertussis toxin (PT) antibody was detected by the indirect enzyme-linked immunosorbent assay (ELISA) method in serum samples from 2,085 healthy subjects ranging in age from 6 months to > or = 60 years. Also included in the evaluation were responses to a questionnaire including sociodemographic characteristics, vaccination, and infection history. Titers of 50-99 ELISA units (EU)/mL and of > or = 100 EU/mL were accepted as indicative for recent exposure or infection. In addition, 30 EU/mL was estimated to be a sufficient titer in women of childbearing age to protect their newborns until administration of their first dose of pertussis vaccine. After the age of 4-5 years, presence of high-titered antibodies that increase with age might be a reflection of circulating infection and indicate the magnitude of the threat to infants. According to the questionnaires, in the groups younger than 15 years old, three to four doses of diphtheria toxoid-whole cell pertussis-tetanus toxoid (DwPT) were administered in 47.2 to 77.4%, 91.2 to 100.0%, and 83.5 to 100.0% of participants in Diyarbakir, Samsun, and Antalya, respectively. In addition, up to half of the expectant mothers we studied lacked a sufficient level of estimated antibody titers. To protect infants from life-threatening pertussis infection, improving vaccination coverage to ensure herd immunity and uniformly establishing coverage throughout the country are essential. Furthermore, revaccination with acellular vaccine for schoolchildren as well as for the households of pregnant women is recommended.

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Section: Measurement Of Immunoglobulin G (Igg) Anti-pt Antibody By Inmentioning

confidence: 99%

Prevalence of high antibody titers of pertussis in Turkey: reflection of circulating microorganism and a threat to infants

Esen¹,

Çöplü²,

Kurtoğlu³

et al. 2007

Clinical Laboratory Analysis

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Assessment of Assay Precision: a Case Study of an ELISA for Anti-pertussis Antibody

Das

1999

Biologicals

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Comparison of five calculation modes for antibody ELISA procedures using pertussis serology as a model

Reizenstein

Hallander

Blackwelder

et al. 1995

Journal of Immunological Methods

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Determination of antibodies to pertussis toxin in working reference preparations of anti-pertussis sera from various national control laboratories

Cited by 7 publications

References 11 publications

Prevalence of high antibody titers of pertussis in Turkey: reflection of circulating microorganism and a threat to infants

Prevalence of high antibody titers of pertussis in Turkey: reflection of circulating microorganism and a threat to infants

Assessment of Assay Precision: a Case Study of an ELISA for Anti-pertussis Antibody

Comparison of five calculation modes for antibody ELISA procedures using pertussis serology as a model

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