Determination of antibiotics using luminescent Escherichia coli and blood serum

Vlasova, Irina I.; Asrieli, Tatyana V.; Gavrilova, E. M.; Danilov, V.S.

doi:10.1134/s0003683807040114

Cited by 7 publications

(2 citation statements)

References 25 publications

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“…Methods based on biochemiluminescent reactions play an important role not only in environmental research, but also in biomedical studies and clinical medicine (Rathinam, Mohanan, 1998;Simon et al, 2001;Kuklin et al, 2003;Gad et al, 2004;Lang et al, 2005;Deriabin, Poliakov, 2005;Vlasova et al, 2007), as well as in immunology (Kurtasova et al, 2001;Edinger et al, 2003). (Pellinen et al, 2002;Lehtinen et al, 2003;Mitchel, Gu, 2004;Yagi et al, 2007, Li et al, 2008.…”

Section: Genetically-modified Organismsmentioning

confidence: 99%

Bioluminescent Bioassays Based on Luminous Bacteria

K.¹,

M.²,

A.³

et al. 2009

J. Sib. Fed. Univ., Biol.

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Bioluminescent analysis is one of the most promising express methods for biologically monitoring the environment because the luminescent system is highly sensitive to even micro quantities of pollutants. Bioassays based on luminous bacteria give an integral estimation of toxicity and frequently surpass other known bioassays in speed, accuracy, sensitivity and simplicity. The enzymes of bacterial luminescent system are also used in developing highly sensitive analytical methods for practical purposes. This paper considers the main features of bacterial bioluminescence and isolated luminescent system, and also the applications of bioluminescence. One part reviews the investigations on the influence of different chemical substances on bacterial luminescence, development and using of bioluminescent bioassays, in particular some of them, in environmental monitoring of several regions in Russia. Luminous bacteria strains from the Culture Collection of Institute of Biophysics SB RAS (CCIBSO 836) are successfully used to produce new or improved bioassays based on lyophilized bacteria bearing the lux gene. Such bioassays are suitable for biotesting of water, air, soil, and the chemical substances used in everyday life. The «know-how» of kits for bioluminescent analysis and bioassays based on lyophilized natural luminous bacteria P.phosphoreum and recombinant Е.соli strain with cloned lux genes is developed. The bioluminescent assay has certificate and is recommended as an additional method of ecological monitoring in parallel with the other bioassays. The sensitivity of the bioassays developed in the IBP SB RAS is comparable to that of the foreign analogues Microtox ©TM , ToxAlert ©TM , etc.

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Section: Genetically-modified Organismsmentioning

confidence: 99%

Bioluminescent Bioassays Based on Luminous Bacteria

K.¹,

M.²,

A.³

et al. 2009

J. Sib. Fed. Univ., Biol.

View full text Add to dashboard Cite

show abstract

“…In 1990 the bioluminescent system of enzymatic coupled reactions was suggested as a toxicity assay (Kratasyuk et al, 1990); its applications were developed (Kudryasheva et al, 2002(Kudryasheva et al, , 2003. Now, the ecological bioassay is a traditional and important biotechnological application of the bioluminescence phenomenon (Girotti et al, 2008;Gitelson, et al, 2002;Roda et al, 2004;, 2006b, Lapota et al, 2008Cali et al, 2008;Vlasova et al, 2007;Reis et al, 2007;Vetrova et al, 2007).…”

Section: Introductionmentioning

confidence: 99%

Bioluminescent Monitoring: Detoxification of Phenol Solutions by Humic Substances and UV-irradiation

S.¹,

S.²,

S.³

et al. 2008

J. Sib. Fed. Univ., Biol.

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The study demonstrates redox activity of humic substances in solutions of organic reducers and oxidizers; a series of homologous 1,4-hydroquinones (phenols) and their oxidized forms (1,4-quinones) was taken as an example. The well-founded interrelation between redox potentials of the quinonephenols pairs and detoxification coefficients in the humic solutions was ascertained. Photoinduced UV detoxification of the phenol solutions was revealed; combined effect of humic substances and UV irradiation in the phenol solutions was studied as well. Detoxification coefficients were calculated. A conclusion was made that UV-irradiation and treatment by humic substances reveal non-additive but antagonistic effect.

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Detoxification of AM-241 solutions by humic substances: bioluminescent monitoring

et al. 2010

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The study addresses the effect of humic substances on marine luminous bacteria Photobacterium phosphoreum exposed to Am-241 (3,000 Bq L(-1), water solution). Luminescent intensity of the bacteria was applied as a marker of their physiological activity. Humic substances have been found to reduce the effect of Am-241 on luminescence, decrease damage to cells, and change distribution of Am-241 between bacterial cells and intercellular media. It was shown that water-soluble humic substances, being products of natural transformation of organic substances in soil and bottom sediments, can serve as protecting agents for water microorganisms exposed to alpha radionuclides.

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Determination of antibiotics using luminescent Escherichia coli and blood serum

Cited by 7 publications

References 25 publications

Bioluminescent Bioassays Based on Luminous Bacteria

Bioluminescent Bioassays Based on Luminous Bacteria

Bioluminescent Monitoring: Detoxification of Phenol Solutions by Humic Substances and UV-irradiation

Detoxification of AM-241 solutions by humic substances: bioluminescent monitoring

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