Determination of antibiotic resistance genes and virulence factors in Escherichia coli isolated from Turkish patients with urinary tract infection

Abstract

Introduction: Escherichia coli ranks among the most common sources of urinary tract infections (UTI). Methods: Between November 2015 and August 2016, 90 isolates of E. coli were isolated from patients at Rize Education and Research Hospital in Turkey. Antibiotic susceptibility was determined for all isolates using the Kirby-Bauer disk diffusion method. These E. coli isolates were also screened for virulence genes, β-lactamase coding genes, quinolone resistance genes, and class 1 integrons by PCR. Results: With respect to the antibiotic resistance profile, imipenem and meropenem were effective against 98% and 90% of isolates, respectively. A high percentage of the isolates showed resistance against β lactam/β lactamase inhibitor combinations, quinolones, and cephalosporins. PCR results revealed that 63% (57/90) of the strains carried class 1 integrons. In addition, a high predominance of extended-spectrum β-lactamases (ESBLs) was observed. The qnrA, qnrB, and qnrS genes were found in 24 (26.6%), 6 (6.6%), and 3 (3.3%), isolates, respectively. The most common virulence gene was fim (82.2%).The afa, hly, and cnf1 genes were detected in 16.6%, 16.6%, and 3.3% of isolates, respectively. Moreover, we observed eleven different virulence patterns in the 90 E. coli isolates. The most prevalent pattern was fım, while hly-fım, afa-aer-cnf-fım, aer-cnf, afa-aer, and afacnf-fım patterns were less common. Conclusions: Most of the E. coli virulence genes investigated in this study were observed in E. coli isolates from UTI patients. Virulence genes are very important for the establishment and maintenance of infection.

Antimicrobial resistant and virulence genes profiles of some Gram-negative bacteria from clinical isolates at Usmanu Danfodiyo University Teaching Hospital, Sokoto, Nigeria

“…This will serve as a means or a strategy for survival in all conditions the organisms may find themselves. Rahdar et al (2015), and Duzgun et al (2019), also reported similar results in their studies [36,37]. Of the other genes for adhesion, papC was found to be relatively higher than sfaS.…”

Virulence factors and antimicrobial resistance of uropathogenic Escherichia coli (UPEC) isolated from urinary tract infections: a systematic review and meta-analysis

“…Key virulence factors involved in the pathophysiology of UTIs function in invasion, colonization and mediation of host defences subversion [ 8 ]. PAIs furthermore often carry cryptic or functional genes that encode mobility factors, such as integrases, transposases and insertion sequence elements [ 7 ], which are traces from their mobile history and may promote and contribute to the spread and emergence of antimicrobial resistance [ 9 – 12 ].…”

Phylogenetic Group/Subgroups Distributions, Virulence Factors, and Antimicrobial Susceptibility of Escherichia coli Strains from Urinary Tract Infections in Hatay

“…Previously, there have been a few studies featuring virulence properties, antibiotic resistance, and its relationship with phylogenetic groups among E. coli associated with UTIs in Turkey [10][11][12][13] . Therefore, the main objective of this study was to determine the antimicrobial susceptibility, phylogeny, and virulence genes of E. coli isolated from patients admitted to Hatay State Hospital with UTI complaint.…”