Determination of antibiotic residues in Indian honeys and assessment of potential risks to consumers

Kumar, Atul; Gill, Jatinder Paul Singh; Bedi, Jasbir Singh; Chhuneja, P. K.; Kumar, Amit

doi:10.1080/00218839.2019.1677000

Cited by 27 publications

(13 citation statements)

References 28 publications

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“…They reported that erythromycin antibiotic residue in positive samples was in the range of 50-112 ng/g. Similar to Kumar et al [19], in our study, the mean erythromycin antibiotic residue of the G and F brands was the same, and the H brand had high erythromycin antibiotic residue. They stated that the pharmacologically active substances in their studies are widely used in veterinary medicine, and therefore, environmental contamination (spread with urine and feces) becomes possible.…”

Section: Discussionsupporting

confidence: 90%

“…When compared with the values of Bonerba et al [29] and Savarino et al [30], erythromycin antibiotic residue was found to be high, and streptomycin antibiotic residue was found to be low in our study. Kumar et al [19] reported that eight (5.3%) out of 150 honey samples in India were positive for erythromycin residues, and they found the mean ± SD to be 78.8 ± 23.6 ng/g. They reported that erythromycin antibiotic residue in positive samples was in the range of 50-112 ng/g.…”

Section: Discussionmentioning

confidence: 99%

“…These antibacterial drugs can be used for prophylactic or therapeutic purposes to treat disease in animals and as growth-promoting agents in subtherapeutic concentrations [14][15][16][17]. The use of antibiotics in animals can create residues in foods of animal origin, such as meat and honey [18,19]. Antibiotics can be found in honey, as they are used in beekeeping for the treatment of bacterial diseases.…”

Section: Introductionmentioning

confidence: 99%

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Detection of Antibiotic Residues in Blossom Honeys from Different Regions in Turkey by LC-MS/MS Method

Demirhan

2022

Antibiotics

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In the present study, a total of 80 commercial blossom honey samples were obtained from local markets in Ankara, Turkey. These honeys were analyzed for 35 important and risky antibiotics (sulfonamide, tetracycline, macrolide, cephalosporin, aminoglycoside, quinolone, nitrofuran, chloramphenicol, and anthelmintic groups) by the LC-MS/MS multi-antibiotic method. In addition to these analyses, pH measure, moisture, and electrical conductivity were determined in these honey samples. Finally, seven out of 35 antibiotic residues investigated in the honeys were positive. The most frequently detected antibiotics in the analyzed samples were dihydrostreptomycin, streptomycin, erythromycin, sulfadimidine (sulfamethazine), and enrofloxacin as 58.75%, 22.5%, 13.75%, 10%, and 2.5%, respectively. Tetracycline and doxycycline were detected in only one sample. The pH, moisture, and electrical conductivity values of the honey samples were determined as between pH 3.78 and 5.41, 17.48 and 18.03%, and 0.25 and 0.47 mS/cm, respectively. In terms of food safety and human health, it is very important to monitor the residues of these pharmacologically active substances with analytical methods.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Detection of Antibiotic Residues in Blossom Honeys from Different Regions in Turkey by LC-MS/MS Method

Demirhan

2022

Antibiotics

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“…The presence of antibiotic residues in honey present a risk to the health of consumers, because they could be a source of allergic reactions (Toldra and Reig, 2006) and can lead to obtaining bacterial resistant strains to antibiotics after consumption of honey (Bargańska et al, 2011). That is why, in recent years, several publications have focused on the determination of antimicrobial contaminants in beekeeping products especially honey (Kumar et al, 2020;Savarino et al, 2020).…”

Section: Relationship Among Antibiotic Residues and Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O Berg) Frommentioning

confidence: 99%

Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

et al. 2020

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Antibiotic-resistant bacteria continue to be of major health concern worldwide. In recent years, several reports and scientific articles claim the contamination of honey by antibiotics, detectable concentrations of antibiotic residues in honey are illegal. They, may cause hypersensitivity or resistance to drug therapy in humans, and are perceived by consumers as undesirable. In this sense, the purpose of this work was to examine the antibacterial activity of the Euphorbia resinifera (E. resinifera) honey against Escherichia coli and Staphylococcus aureus in vitro using the well-agar diffusion assay followed by dilution range to obtain more precise minimum inhibitory concentration values. The second aim is to evaluate the presence of antibiotics in honey using a screening test: Evidence InvestigatorTM, an immuno-enzymatic method for detection of 27 antibiotic residues followed by a liquid chromatography-tandem mass spectrometry (LC-MS/MS) for confirmation of suspect samples; in order to assess the relationship between the presence of antibiotic residues and the antibacterial activity of honey. In this study, a total of 37 E. resinifera honey samples were analyzed. The results show that all samples of honey inhibited the growth of bacteria at the dilutions at 50% (v/v); the highest inhibition zone (25.98 ± 0.11 mm) was recorded from sample 5 for Staphylococcus aureus and (13.84 ± 1.10 mm) in sample 17 for Escherichia coli and that 50% (v/v) dilutions showed significant antibacterial effect compared to other dilutions (6.25, 12.5, 25% (v/v)). In all samples, there were no antibiotic residues detected except for one showing the detection of Trimethoprim at 6.48 µg kg-1. Our research is one of the first studies that relate the he relationship between the presence of antibiotic residues and the antibacterial activity of Euphorbia resinifera honey and showed that the antibacterial activity of honey might be due to the high osmotic nature, a low pH, its content of phenolic compounds and hydrogen peroxide and also to its content of methylglyoxal.

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“…Previous studies have described microbiological and thin-layer chromatography methods for monitoring VAs in biological matrices [ 6 , 7 , 8 ]; however, most of those studies have focused on one group of VAs only. Several recent studies used HPLC for TCs, sulfonamides, and FQs quantification in other animals’ matrices [ 9 , 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

Determination of Tetracycline, Oxytetracycline, Sulfadiazine, Norfloxacin, and Enrofloxacin in Swine Manure Using a Coupled Method of On-Line Solid-Phase Extraction with the UHPLC–DAD

Gaballah

Zhang

et al. 2021

Antibiotics

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The use of various veterinary antibiotics (VAs) in animal husbandry raises serious concerns about the development of antibiotic resistance. Antibiotics such as tetracycline, oxytetracycline, sulfadiazine, norfloxacin, and enrofloxacin are the most frequently used antimicrobial compounds in animal husbandry and generate large eco-toxicological effects; however, they are still difficult to determine in a complex matrix such as swine manure. This study has developed an effective method for detecting five VAs in swine manure using Ultra-High-Performance Liquid Chromatography–Diode Array Detector (UHPLC–DAD) coupled with on-line solid-phase extraction (SPE). The results show that the mobile phase of ACN/0.01 M oxalic acid was the optimum at pH 3.0. VAs in a swine manure matrix were extracted using solid extraction buffer solution (T3) with 97.36% recovery. Sensitivity, accuracy, and precision were also evaluated. The validity study showed good linearity (R2 > 0.99). Limit of detection (LOD) was found to be from 0.1 to 0.42 µg mL−1 in the liquid fraction and from 0.032 to 0.58 µg g−1 dw in the solid fraction. The corresponding values of the limit of quantification (LOQ) ranged from 0.32 to 1.27 µg mL−1 for the liquid fraction and from 0.096 to 1.77 µg g−1 dw for the solid fraction. Therefore, the proposed method showed the potential applicability for detecting different antibiotic compounds from swine manure samples.

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Determination of antibiotic residues in Indian honeys and assessment of potential risks to consumers

Cited by 27 publications

References 28 publications

Detection of Antibiotic Residues in Blossom Honeys from Different Regions in Turkey by LC-MS/MS Method

Detection of Antibiotic Residues in Blossom Honeys from Different Regions in Turkey by LC-MS/MS Method

Relationship Among Antibiotic Residues And Antibacterial Activity Of The Endemic Spurge Honey (Euphorbia Resinifera O. Berg) From Morocco

Determination of Tetracycline, Oxytetracycline, Sulfadiazine, Norfloxacin, and Enrofloxacin in Swine Manure Using a Coupled Method of On-Line Solid-Phase Extraction with the UHPLC–DAD

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