Determination of antibacterial activity in rhizome of plant Aechmea magdalenae (andre) andre ex baker

Abstract

The rising resistance of microbes toward the present-day antibiotics has led the researchers to increasinglyinvestigate medicinal plant-based antibiotics. The plant Aechmea magdalenae (Andre) Andre ex Baker (Bromeliaceae) is a terrestrial bromeliad native to Central America and Northwestern South America. Throughout much of its range, the juice from the leaves of A. magdalenae has been used by locals as caustic for wounds. Inthe lowland wet forests of Costa Rica, however, the local herbal healers do not rely on this species. In this study,antibacterial and biochemical analyses were conducted on A. magdalenae to document its potential use as amedicinal plant for both the local people in Costa Rica and to the scientific community. Gas chromatography-massspectrometry profiling studies also revealed, acetic acid to be one of the important chemical compound present inhigh probability in A. magdalenae. The antibacterial activity was confirmed by performing agar disc diffusion assayusing various concentrations of acetic acid against bacteria Escherichia coli. Acetic acid clearly demonstratedits antibacterial effect against E. coli. The antibacterial activity of the methanolic extracts of A. magdalenae wastested against Gram-negative (E. coli) and Gram-positive bacteria (Staphylococcus aureus). The methods usedto determine the antibacterial activity of the plant extracts were agar well diffusion assay and 96-well plateassay. In both the assays, gentamicin was used as a positive control and 20% dimethyl sulfoxide was used asa negative control. In 96 well plate assay, resazurin was used as an indicator to evaluate qualitatively whetherthe bacteria there was the growth of the bacteria or no growth. All the tests were performed in triplicates. Thezones of inhibition recorded were higher for S. aureus (10.5 mm) as compared to E. coli (9.5 mm). The minimuminhibitory concentration for E. coli was higher as compared to S. aureus.

To Develop the Method for UHPLC-HRMS to Determine the Antibacterial Potential of a Central American Medicinal Plant

“…The ethnobotanical approach has also proved to be advantageous while finding new drug candidates. GC-MS profiling confirmed acetic acid as a high probability (>90%) chemical compound, out of many others, present in the rhizome of A. magdalenae [4]. The antibacterial potential of acetic acid was confirmed in our laboratory using agar disc diffusion assay performed on gram negative bacteria [4].…”

“…GC-MS profiling confirmed acetic acid as a high probability (>90%) chemical compound, out of many others, present in the rhizome of A. magdalenae [4]. The antibacterial potential of acetic acid was confirmed in our laboratory using agar disc diffusion assay performed on gram negative bacteria [4]. The agar well diffusion assay was performed to evaluate the antibacterial activity while a 96-well plate assay determined the MIC of the rhizome extract of A. magdalenae [4].…”

“…This difference in mass between resazurin's intact and reduced forms was detected by mass spectrometer in positive ion mode. The MIC of plant A. magdalenae was determined previously using a 96-well plate assay [4]. In the present study, a novel rapid LC-MS method was developed that could determine the antibacterial potential of medicinal plants using the mass difference of resazurin and resorufin.…”

“…confirmed in our laboratory using agar disc diffusion assay performed on gram negative bacteria [4]. The agar well diffusion assay was performed to evaluate the antibacterial activity while a 96-well plate assay determined the MIC of the rhizome extract of A. magdalenae [4].…”

“…confirmed in our laboratory using agar disc diffusion assay performed on gram negative bacteria [4]. The agar well diffusion assay was performed to evaluate the antibacterial activity while a 96-well plate assay determined the MIC of the rhizome extract of A. magdalenae [4]. Both the agar well diffusion assay and the 96-well plate assay included gram-negative Escherichia coli (E. coli) and grampositive Staphylococcus aureus (S. aureus) bacteria.…”