Determination of antagonistic effect of bio-agents against cereal damping-off caused by Fusarium graminearum in wheat

Abstract

The antagonistic effect of the isolates of Gliocladium roseum, Saccharomyces cerevisiae, Sordaria fimicola, and their mixtures, at different concentrations against the cereal damping-off pathogen, Fusarium graminearum, was examined in vitro and in vivo (foliar, seed, soil, seed + soil) treatments on the susceptible wheat cultivars "Gun 91 and Sultan 95." The 3 isolates inhibited growth of F. graminearum at a concentration of 1 × 10 9 spores/ml with inhibition rates of 84, 88, and 91%, respectively under in vitro conditions. For in vivo assays, the mixture of S. cerevisiae + S. fimicola exhibited a considerable antagonistic activity even at a concentration of 1 × 10 5 spores/ml. Particularly, at the seed + soil treatment of the mixture, the pathogen was almost completely suppressed with an inhibition rate above 96% at concentrations of 1 × 10 8 and 1 × 10 9 spores/ml for both wheat cultivars, and the percentage of emerged seedlings reached nearly 100%. The results verified that the mixture of S. cerevisiae + S. fimicola had a high potential, as a promising biocontrol agents and an eco-friendly alternative, to be used against the cereal damping-off caused by F. graminearum, to reduce the use of systemic fungicides.

Trichoderma atroviride suppresses Fusarium graminearum by altering primary and secondary metabolite biosynthesis profiling

“…Microbial biological control agents (BCAs) have been frequently used in the fight against Fusarium diseases in recent years (Franco et al, 2011;Nourozian et al, 2006). Several fungal species such as Gliocladium roseum and G. catenulatum may be useful in the in vitro and in vivo control of F. graminearum diseases (Er, 2020;Legrand et al, 2018). Trichoderma spp.…”

“…catenulatum may be useful in the in vitro and in vivo control of F . graminearum diseases (Er, 2020; Legrand et al, 2018). Trichoderma spp.…”