Determination of an antimicrobial activity of Weissella confusa, Lactobacillus fermentum, and Lactobacillus plantarum against clinical pathogenic strains of Escherichia coli and Staphylococcus aureus in co-culture

Shah, Nihir; Patel, Ami; Ambalam, Padma; Holst, Olle; Ljungh, Åsa; Prajapati, J. B.

doi:10.1007/s13213-016-1201-y

Cited by 29 publications

(22 citation statements)

References 28 publications

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“…These results were similar to those found by Coudeyras et al [ 25 ] who, using a L. rhamnosus strain, demonstrated inhibition of G. vaginalis , P. bivia , and C. albicans after 8 hours, with significant inhibition of G. vaginalis after 24 hours. Other pathogens that are also capable of causing bacterial vaginosis, such as E. coli and S. aureus , also have their growth affected when cocultivated with strains of L. plantarum and L. fermentum : after 24 hours, a decrease of up to three logs was observed when compared to controls [ 46 ].…”

Section: Resultsmentioning

confidence: 99%

In VitroActivity of Lactobacilli with Probiotic Potential Isolated from Cocoa Fermentation againstGardnerella vaginalis

Pessoa

Melgaço

Almeida

et al. 2017

BioMed Research International

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Study of the probiotic potential of microorganisms isolated from fermented foods has been increasing, especially studies related to lactobacilli. In intestinal models, lactobacilli have demonstrated beneficial properties, such as anti-inflammatory activity and increased antibody production, but the molecular mechanisms involving probiotic and antagonistic action as well as their effect on human vaginal cells have not yet been fully elucidated. The aim of this study was to evaluate the functional and antagonistic properties of three strains of lactobacilli isolated from cocoa fermentation (Lactobacillus fermentum 5.2, L. plantarum 6.2, and L. plantarum 7.1) against Gardnerella vaginalis. Our results show that the lactobacilli have potential use as probiotics, since they have high hydrophobicity and autoaggregation properties and effectively adhere to vaginal cells. Metabolites secreted into the culture medium and whole cells of the strains under study are capable of interfering with the growth of G. vaginalis to different degrees. The elucidation of the antagonistic mechanisms as well as their effect on human cells may be useful in the development of a product containing such microorganisms or products secreted by them.

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Section: Resultsmentioning

confidence: 99%

In VitroActivity of Lactobacilli with Probiotic Potential Isolated from Cocoa Fermentation againstGardnerella vaginalis

Pessoa

Melgaço

Almeida

et al. 2017

BioMed Research International

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“…The culture of S. Typhi without CFCS served as the control group. All plates were incubated at 37 C for 24-48 h (Shah et al, 2016). The number of viable cells were counted and calculated as the percentage of Salmonella survival with a given formula; % Salmonella survival ¼ ðViable counts in treatment=Viable counts in controlÞ Â 100…”

Section: Influence Of Cfcs On Salmonella Growth and Survivalmentioning

confidence: 99%

“…The antagonistic activities of W. confusa on pathogenic bacteria including Bacillus cereus, Escherichia coli, Helicobacter pylori, Proteus mirabilis, Pseudomonas aeruginosa, and Staphylococcus aureus, have been described in several previous reports. W. confusa can produce organic acids and bacteriocins in culture medium and some strain can strongly adhere to intestinal epithelial cells (Chavasirikunton, Vatanyoopaisarn & Phalakornkule, 2006;Lee, 2004;Nam et al, 2002;Purkhayastha et al, 2017;Shah et al, 2016). An increasing number of LAB and metabolite related, studies only focus on the properties of other LAB rather than Weissella spp.…”

Section: Introductionmentioning

confidence: 99%

Organic acids and 2,4-Di-tert-butylphenol: major compounds of Weissella confusa WM36 cell-free supernatant against growth, survival and virulence of Salmonella Typhi

Pelyuntha

Chaiyasut

Kantachote

et al. 2020

PeerJ

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Background Salmonella Typhi (S. Typhi), the causative agent of typhoid fever, causes serious systemic disease in humans. Antibiotic treatment is required for the S. Typhi infection, while the inappropriate use of antibiotics causes increased drug-resistant S. Typhi. Hence, alternative therapies through non-antibiotic approaches are urgently needed. The use of beneficial lactic acid bacterium and/or its metabolites to control typhoid fever represent a promising approach, as it may exert protective actions through various mechanisms. Method In this study, the cell-free culture supernatant (CFCS) of Weissella confusa WM36 was evaluated via the antibacterial activity, and its metabolites were identified. In addition, the effects of CFCS on Salmonella virulence behaviors were also investigated. Result Based on strong inhibition the growth of S. Typhi DMST 22842, organic acids (lactic acid and acetic acid) and 2,4-Di-tert-butylphenol (2,4 DTBP), were the main antibacterial metabolites presented in CFCS of strain WM36. Minimum inhibitory concentration (MIC) at 40% WM36–CFCS dramatically reduced the S. Typhi population to more than 99.99% at 4 h and completely inhibited biofilm formation, while sub-MIC at 20% (v/v) and MIC could reduce 100% of motility. Additionally, sub-MIC at only 10% (v/v) WM36–CFCS did down-regulate the expression of virulence genes which are responsible for the type-III secretion system, effector proteins, and quorum sensing system in this pathogen. Conclusion W. confusa WM36 and its metabolites are shown to be a promising candidates, and an effective approach against typhoid Salmonella burden.

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“…The culture of S. Typhi without CFCS served as the control group. All plates were incubated at 37 ºC for 24-48 h (Shah et al, 2016). The number of viable cells were counted and calculated as the percentage of Salmonella survival with a given formula; % Salmonella survival = (Viable counts in treatment/ Viable counts in control) x100.…”

Section: Gas Chromatography-mass Spectrophotometry (Gc-ms)mentioning

confidence: 99%

Peer Review #2 of "Organic acids and 2,4-Di-tert-butylphenol: major compounds of Weissella confusa WM36 cell-free supernatant against growth, survival and virulence of Salmonella Typhi (v0.2)"

2020

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Background. Salmonella Typhi (S. Typhi), the causative agent of typhoid fever, causes serious systemic disease in humans. Antibiotic treatment is required for the S. Typhi infection, while the inappropriate use of antibiotics causes increased drug-resistant S. Typhi. Hence, alternative therapies through nonantibiotic approaches are urgently needed. The use of beneficial lactic acid bacterium and/or its metabolites to control typhoid fever represent a promising approach, as it may exert protective actions through various mechanisms. Method. In this study, the cell-free culture supernatant (CFCS) of Weissella confusa WM36 was evaluated via the antibacterial activity, and its metabolites were identified. In addition, the effects of CFCS on Salmonella virulence behaviors were also investigated. Result. Based on strong inhibition the growth of S. Typhi DMST 22842, organic acids (lactic acid and acetic acid) and 2,4-Di-tert-butylphenol (2,4 DTBP), were the main antibacterial metabolites presented in CFCS of strain WM36. Minimum inhibitory concentration (MIC) at 40% WM36-CFCS dramatically reduced the S. Typhi population to more than 99.99% at 4 h and completely inhibited biofilm formation, while sub-MIC at 20% (v/v) and MIC could reduce 100% of motility. Additionally, sub-MIC at only 10% (v/v) WM36-CFCS did down-regulate the expression of virulence genes which are responsible for the type-III secretion system, effector proteins, and quorum sensing (QS) system in this pathogen. Conclusion. W. confusa WM36 and its metabolites are shown to be a promising candidates, and an effective approach against typhoid Salmonella burden.

show abstract

Determination of an antimicrobial activity of Weissella confusa, Lactobacillus fermentum, and Lactobacillus plantarum against clinical pathogenic strains of Escherichia coli and Staphylococcus aureus in co-culture

Cited by 29 publications

References 28 publications

In VitroActivity of Lactobacilli with Probiotic Potential Isolated from Cocoa Fermentation againstGardnerella vaginalis

In VitroActivity of Lactobacilli with Probiotic Potential Isolated from Cocoa Fermentation againstGardnerella vaginalis

Organic acids and 2,4-Di-tert-butylphenol: major compounds of Weissella confusa WM36 cell-free supernatant against growth, survival and virulence of Salmonella Typhi

Peer Review #2 of "Organic acids and 2,4-Di-tert-butylphenol: major compounds of Weissella confusa WM36 cell-free supernatant against growth, survival and virulence of Salmonella Typhi (v0.2)"

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