Determination of Amitraz in Cattle Dipping Baths and Sprays

Abstract

Infrared (IR) and gas chromatographic methods have been developed for determining the acaricide amitraz (N-(2,4-dimethylphenyl)-N - [[(2,4- dimethylphenyl)imino]methyl] - N - methanimidamide) in cattle dip solutions. Extraction with petroleum ether, followed by IR and gas-liquid chromatography (GLC) gave coefficients of variation of 3.4 and 3.6%, respectively, for 0.010-0.035% amitraz. Dilution of the dip sample with acetone and determination by GLC gave a coefficient of variation of 3.6% in the same range. Recovery of amitraz from fortified dip samples was 97–100%.