Determination of aminothiols by liquid chromatography with amperometric detection at a silver electrode: Application to white wines

Sarakbi, Ahmad; Aydoğmuş, Zeynep; Dago, Àngela; Mertens, Dominique; Dewert, Jean-Yves; Kauffmann, Jean‐Michel

doi:10.1016/j.aca.2013.04.070

Cited by 17 publications

(29 citation statements)

References 47 publications

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“…Quantitative and qualitative analyses of the amino-thiols present in grapes and wine have been reported; compounds found include GSH, N-acetylcysteine, Cys and omoCys, especially in white wines [18]. These compounds play an important role in slowing down the browning process due to natural oxidation.…”

Section: Principal Non-alcoholic Components Of Winementioning

confidence: 99%

Wine consumption and intestinal redox homeostasis

et al. 2014

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Regular consumption of moderate doses of wine is an integral part of the Mediterranean diet, which has long been considered to provide remarkable health benefits. Wine׳s beneficial effect has been attributed principally to its non-alcoholic portion, which has antioxidant properties, and contains a wide variety of phenolics, generally called polyphenols. Wine phenolics may prevent or delay the progression of intestinal diseases characterized by oxidative stress and inflammation, especially because they reach higher concentrations in the gut than in other tissues. They act as both free radical scavengers and modulators of specific inflammation-related genes involved in cellular redox signaling. In addition, the importance of wine polyphenols has recently been stressed for their ability to act as prebiotics and antimicrobial agents.Wine components have been proposed as an alternative natural approach to prevent or treat inflammatory bowel diseases. The difficulty remains to distinguish whether these positive properties are due only to polyphenols in wine or also to the alcohol intake, since many studies have reported ethanol to possess various beneficial effects. Our knowledge of the use of wine components in managing human intestinal inflammatory diseases is still quite limited, and further clinical studies may afford more solid evidence of their beneficial effects.

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Section: Principal Non-alcoholic Components Of Winementioning

confidence: 99%

Wine consumption and intestinal redox homeostasis

et al. 2014

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“…This was confirmed by cyclic voltammetry of a solution of cysteine at the AgCPE (Figure ). The interaction of the silver composite electrode, polarized at 0.08 V vs. Ag/Ag + , with Cys produced an oxidation wave close to 0.00 Volt corresponding to the catalytic oxidation of silver (particles) at the AgCPE . No oxidation wave was obtained by CV of 2‐DPDS in the potential range investigated other than an increase of the AgCPE capacitive current.…”

Section: Resultsmentioning

confidence: 99%

Determination of Thiols and Free Thiol Content in a Protein with Liquid Chromatography Coupled to Amperometric Detection at a Silver Based Carbon Paste Electrode

2016

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Liquid chromatography (LC) coupled to an electrochemical detector was applied for the determination of small thiols such as cysteine (Cys), glutathione (GSH) and dithiothreitol (DDT) and the free thiol content in a protein. The thio‐compounds were first derivatized by 2,2′‐dipyridyldisulfide (DPDS) at pH 4.5 to yield the corresponding disulfide and 2‐thiopyridone (2‐TP). The reaction mixture was separated isocratically on a reversed‐phase C18 column with amperometric detection (AD) at a carbon paste electrode modified with silver microparticles and polarized at 0.080 V versus Ag/AgCl. The LC‐AD response corresponding to the generated 2‐TP allowed for the indirect determination of the studied thiol derivatives in the 1×10−5–1×10−4 M range for Cys and GSH and 5×10−6–5×10−5 M range for DTT. The LOD and LOQ values were in the range of 9×10−7–4×10−6 M and 3×10−6−‐5.5×10−6 M, respectively for the three studied compounds. Application to the determination of the free thiol content of human and bovine serum albumin as model proteins was realized. The LC‐AD results were validated with respect to a developed spectrophotometric method performed under identical experimental derivatization conditions.

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“…Both the USP and European Pharmacopeia apply LC with (PAD) at a gold working electrode for the test of composition and related substances for antimicrobial compounds such as gentamicin, framycetin, amikacin, kanamycin, and tobramycin. A silver-based amperometric working electrode is the material of choice for halides, cyanide, thiocyanate, sulfide [40][41][42], and thiol-based compounds such as captopril, acetylcysteine, or aminothiols such as cysteine, glutathione, homocysteine, etc., with quantification limits in the submicromolar concentration range [6,43,44]. LC with carbon-based detectors such as those based on BDD, thanks to the high positive potentials applied, have been successful for the quantification of both oxidized and reduced forms of aminothiols [45].…”

Section: Basic Analytical Strategy For Implementing An Amperometric Dmentioning

confidence: 99%

Electrochemical and Hyphenated Electrochemical Detectors in Liquid Chromatography and Flow Injection Systems for Drug Compound Analysis

Özkan

Kauffmann

Zuman

2015

Monographs in Electrochemistry

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Determination of aminothiols by liquid chromatography with amperometric detection at a silver electrode: Application to white wines

Cited by 17 publications

References 47 publications

Wine consumption and intestinal redox homeostasis

Wine consumption and intestinal redox homeostasis

Determination of Thiols and Free Thiol Content in a Protein with Liquid Chromatography Coupled to Amperometric Detection at a Silver Based Carbon Paste Electrode

Electrochemical and Hyphenated Electrochemical Detectors in Liquid Chromatography and Flow Injection Systems for Drug Compound Analysis

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