Determination of Aminophenol Isomers by High-Speed Liquid Chromatography

Abstract

The simultaneous quantitation of aminophenol isomers (p-, m- and o-aminophenol) and anilline, which with previously reported methods was difficult and/or complicated, has been performed using a high-speed liquid chromatographic method. The resolutions of chromatograms were appropriate for separation and quantitation, when analysis times were 20 minutes. Since the method is applicable in aqueous media, a successful quantitation of p- and o-aminophenol formed by cytochrome P-450 model systems was carried out.

Determination of 4-aminophenol using the quartz crystal microbalance sensor

“…HPLC determination of 4-AP with spectrophotometric [5][6][7] and fluorescence 8 detection has been reported in the literature. Although the detection limit of 4-AP in water using HPLC was very good (3 mg L 21 ), 8 the method requires expensive instrumentation, and highly trained technical staff to perform the analysis.…”

“…In addition, the analysis can only be done in the laboratory. In some cases, [5][6][7] sample preparation is also needed. Electrochemical, 9,10 colorimetric 4,11,12 and spectrophotometric [13][14][15] methods have also previously been reported.…”