Determination of aminoglycoside antibiotics using an on-chip microfluidic device with chemiluminescence detection

Abstract

We describe an on-chip microflow injection (μFI) approach for the determination of aminoglycoside antibiotics using chemiluminescence (CL) detection. The method is based on the inhibition of the Cu(II)-catalyzed CL reaction of luminol and hydrogen peroxide by the aminoglycosides due to the formation of a complex between the antibiotic and Cu(II). The main features of the method include small sample volumes and a fast response. Syringe pumps were used to insert the sample and the reagents into the microfluidic device. CL was collected using a fiber optic bundle connected to a luminescence detector. All instrumental, hydrodynamic and chemical variables involved in the system were optimized using neomycin as the aminoglycoside model. Inhibition is proportional to the concentration of the antibiotics. The dynamic ranges of the calibration graphs obtained for neomycin, streptomycin and amikacin are 0.3-3.3, 0.9-13.7, and 0.8-8.5 μmol L −1 , and the detection limits are 0.09, 0.28 and 0.24 μmol L −1 , respectively. The precision of the methods, expressed as relative standard deviation, is in the range from 0.8 to 5.0 %. The method was successfully applied to the determination of neomycin in water samples, with recoveries ranging from 80 to 120 %.

Usefulness of Hybrid Magnetoliposomes for Aminoglycoside Antibiotic Residues Determination in Food Using an Integrated Microfluidic System with Fluorometric Detection

“…A comparison of the proposed method features with those of other previously reported methods for AAG determination is shown in Table . As can be seen, few methods used microfluidic systems , or liposomes, , and no methods used the combination of both methods for the AAGs determination. The LOD reached using a microfluidic system with liposomes magnetically retained is lower than those obtained using these methodologies separately.…”

“…For the off-chip coupling, several approaches have been described. The use of bifurcated optical fiber guides or x – y – z alignment devices involved studying a high number of variables, apart from the requirement of voluminous and costly equipment for the coupling. , An alternative is an off-chip option involving integrating the microfluidic chip focused on the optical pathway of a conventional detector using 3D-printed alignment devices …”

“…Since residual AAGs can be found in animal-derived foods, the European Union has established maximum residue limits (MLRs) for these drugs, as shown in Table . Several methods have been described to determine AAGs in food, mainly using chromatography, capillary electrophoresis, and immunoassay. − However, there are few methods using flow or microflow techniques. ,− Fluorimetry is frequently used as a detection method, but these compounds do not present native fluorescence. Diverse derivative reagents have been used for this purpose .…”

Electrochemical Aptasensors for Antibiotics Detection: Recent Achievements and Applications for Monitoring Food Safety

“…For field applications, microfluidics or paper-based chemical assays can also be used. For example, Sierra et al [ 37 ] reported a microfluidic system that measures chemiluminescent output related to the interaction between aminoglycoside antibiotics and copper ions on a chip. The limit of detection for this method was below 1 ng/mL.…”

Flow‐injection chemiluminescence determination of gentamicin: optimization by central composite design

“…Luminol-H 2 O 2 -Cu(II) is a well-known CL reaction (28). This reaction has been exploited for the determination of several aminoglycoside antibiotics including amikacin, streptomycin, kanamycin B, paromomycin and neomycin B (29)(30)(31).…”