Determination of Aminoglycoside Antibiotics by a Colorimetric Method Based on the Aggregation of Gold Nanoparticles

Abstract

A sensitive and selective colorimetric biosensor for determination of gentamicin, amikacin and tobramycin was proposed with the unmodi¯ed gold nanoparticles (GNPs) as the sensing element. Gentamicin, amikacin and tobramycin can rapidly induce the aggregation of gold nanoparticles and is accompanied by a color change from red to blue. The concentration of gentamicin, amikacin and tobramycin can be determined by using UV-Vis spectrometer. The experimental parameters were optimized with regard to pH, incubation time and the concentration of the GNPs. Under optimal experimental conditions, the linear range of the colorimetric sensor for gentamicin/amikacin/tobramycin were 2.67À33.93 ng mL À1 , 13.33À66.67 ng mL À1 and 20À180 ng mL À1 , respectively. The corresponding limit of detection (3) was 0.354 ng mL À1 , 0.999 ng mL À1 and 0.579 ng mL À1 , respectively. This assay was simple and used to detect aminoglycoside antibiotics in milk and medicine products.

Incorporating Fullerenes in Nanoscale Metal–Organic Matrixes: An Ultrasensitive Platform for Impedimetric Aptasensing of Tobramycin

“…The aptasensor shows a remarkable detection performance toward TOB with a limit of detection (LOD) of ∼0.176 pg·mL –1 (∼0.377 pmol·L –1 ) by comparing it with some previously reported materials and test methods (Table ). − By referring to earlier literature studies, − , the prepared UiO-66-NH 2 nanoparticles not only have abound Zr­(IV) sites to immobilize aptamers via the coordination interaction but also possess various functional sites, such as −NH 2 and conjugate skeleton, to interact with the biological base of aptamers. Subsequently, the fixed aptamers have a specific affinity force toward the target TOB molecule, resulting in the highly selective and sensitive sensing performance.…”

Chlortetracycline-Functionalized Silver Nanoparticles as a Colorimetric Probe for Aminoglycosides: Ultrasensitive Determination of Kanamycin and Streptomycin

“…Figure 4b shows a linear response of ratiometric results over the picomolar concentrations of kanamycin (120-480 nM), wherein the linear equation was y = 0.0006x + 0.0167, the R 2 was 0.997, and the calculated LOD was about 160 pM. According to the maximum residue limits of some AMG antibiotics in water, the LOD value obtained shows that the developed AgNPs probe can be used to sensitively detect both streptomycin [26][27][28] and kanamycin in water [29][30][31][32]. A comparison of the kanamycin LOD value and linear range reported for different methods are listed in Table 2.…”

“…AMG antibiotics are challenging to detect because they lack spectroscopic and electrochemical properties. Of the various analytical methods that have been proposed for these antibiotics, colorimetric or spectrophotometric detection using dyes [25] and metallic NPs, notably AuNPs and AgNPs [26][27][28][29], are the simplest methods. This method is based on the polycationic nature of the aminoglycoside, together with negatively-charged AgNPs.…”

Avaliação Da Resposta Óptica Decorrente Da Interação Entre Gentamicina E Nanopartículas De Ouro Esféricas E Em Forma De Bastão Visando Aplicação Analítica

“…Como descrito na seção anterior, os estudos mostraram que a resposta espectral das dispersões de AuNPs-E variam de forma proporcional em relação à quantidade de sulfato de gentamicina, corroborando os resultados reportados por ( WANG et al, 2013;RADHAKUMARY;SREENIVASAN, 2014;SANTOS, 2014 Cabe ressaltar que para a maioria dos excipientes, as proporções molares gentamicina:excipente iguais a 1:1 ou mesmo 1:0,1 são muito acima da proporção esperada nos medicamentos, ou seja, a seletividade da sonda em relação á presença da maioria dos excipientes orgânicos e inorgânicos é elevada. O resultado do teste t calculado a partir dos valores alcançados no comprimento de onda 526 nm foram 2,0; 2,0; 1,7; para os percentuais de recuperação das amostras A, das amostras B e das amostras controle C, no devido ordem.…”