Determination of Aluminium in Human Tissues by Flameless Atomic Absorption Spectroscopy and Comparison of Reference Values

Abstract

SUMMARY.Aluminium in human tissues has been determined by flameless atomic absorption spectroscopy (AAS). Tissues were dried at l lff'C and digested with concentrated nitric acid at SO°C overnight. After dilution with distilled water the samples were measured. The aluminium levels of the controls (healthy individuals who died as a result of an accident) appeared to be: grey matter 2·1 ± 1·0 (mean±SD), white matter 1·7±0·S, spinal cord 3·3±1·S, kidney 1·9±0·7, heart 2·1±1·1, vertebral cortex 1·9±1·8, and vertebral trabeculae 3·1±1·8 Ilg/g dry weight. For a patient with dialysis motor neuropathy significantly higher values were found. Comparison with values in the literature shows that our reference values are in agreement with published results obtained by flameless atomic absorption spectroscopy.

Measurement of aluminum in neuronal tissues using electrothermal atomization atomic absorption spectrophotometry

“…These binuclear (silver-lanthanide) reagents induce substantial changes in the chemical shifts of very weak Lewis bases such as olefins (5, 6, 9,10), aromatics (6-8,10), heteroaromatics (10-12), and halogenated hydrocarbons (6). Chiral binuclear shift reagents have also been reported (13, 14), and these are capable of differentiating between enantiomeric unsaturated hydrocarbons. Ordinary lanthanide shift reagents require the presence of an oxygenor nitrogen-containing functional group, so the unique complexing ability of BLSRs makes them potentially valuable reagents for the analysis of hydrocarbons and hydrocarbon mixtures.…”

“…While existing papers describe a number of techniques designed for service clinical laboratories for measuring Al in serum using EAAAS (5)(6)(7)(8)(9)(10)(11)(12)(13)(14), none of these papers provides sufficient analytical data to demonstrate the validity of Al measurement for neuronal tissues. With the availability of National Bureau of Standards Standard Reference Materials (NBS SRM) for which certified Al concentrations exist, these 0003-2700/86/0358-1744$01.50/0 concerns can now be addressed.…”

Sodium ferrous citrate does not cause aluminum retention in rats with experimentally induced renal failure

“…Thus, the use of E0708 to treat patients with renal anemia who develop iron deficiency during rHuEPO therapy must be explored in regard to gastrointestinal Al absorption and Al retention . In the present study, to examine whether E0708 promotes Al retention in renal failure, we orally administered Al(OH)3 with or without E0708 to s/6-nephrectomized rats [18,19] for 16 weeks, and determined the Al content in the serum, liver, cerebral cortex, and femoral bone by flameless atomic absorption spectroscopy [20] .…”