Determination of agitation and aeration conditions for scale-up of cellulolytic enzymes production by Trichoderma inhamatum KSJ1

Seo, Jin-Ho; Li, Hongxian; Kim, Myong-Jun; Kim, Seong-Jun

doi:10.1007/s11814-007-0044-4

Cited by 8 publications

(3 citation statements)

References 9 publications

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“…For k L a determinations without cells in flasks and air-lift, data were processed as described by Seo et al (2007), establishing constant dissolved oxygen concentration at 8.2 mg L −1 (considering 25 • C and 1 atm). In the case of air-lift the electrode was set at the middle of the liquid column in the air-lift's vessel, between riser and downcomer sections.…”

Section: Volumetric Oxygen Transfer Coefficient (K L A) Determinationsmentioning

confidence: 99%

Development of grapevine somatic embryogenesis using an air-lift bioreactor as an efficient tool in the generation of transgenic plants

Tapia

Sequeida

Castro

et al. 2009

Journal of Biotechnology

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Section: Volumetric Oxygen Transfer Coefficient (K L A) Determinationsmentioning

confidence: 99%

Development of grapevine somatic embryogenesis using an air-lift bioreactor as an efficient tool in the generation of transgenic plants

Tapia

Sequeida

Castro

et al. 2009

Journal of Biotechnology

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“…Microbial enzymatic hydrolysis is generally considered better than chemical hydrolysis because of high product specificity, simplicity of preparation procedures, and low environmental contamination [2]. As the development of industrial enzymes has depended heavily on the use of microbial sources [3], scientists are paying much more attention on it and new sources of enzymes such as cellulolytic enzymes by Trichoderma inhamatum KSJ1 [4] chitinase by Aeromonas sp. DYU-Too7 [2], Lipase by Candida rugosa [5] and milk-clotting aspartic proteinases by Centaurea calcitrapa [6] have been reported recently.…”

Section: Introductionmentioning

confidence: 99%

Purification and partial characterization of milk-clotting enzyme extracted from glutinous rice wine mash liquor

Wang

Cheng

Ahmed

et al. 2009

Korean J. Chem. Eng.

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Glutinous rice wine mash liquor is a traditional food of south of China and its ability to coagulate the milk has been proved. The aim of this work was to extract milk-clotting enzyme from glutinous rice wine mash liquor. A partial purified extract of enzyme was obtained by fractional precipitation with (NH 4 ) 2 SO 4 . The fractions obtained by precipitation, 40-90% possessed the milk-clotting activity (MCA) (145.72 U/mg). The 40-90% (NH 4 ) 2 SO 4 fraction was further purified by sephadex G-100 and DEAE-sephadex A-50 with MCA (4,360±50 U/mg), which was confirmed by SDS-PAGE that showed only one band with a molecular mass of 36.0 kDa. Highest MCA was attained at 36 o C. The enzyme was completely inactivated by heating for 20 min at 60 o C. The MCA increased with the decreasing of milk pH from 8.0 to 5.5, and it was active at the wide range of pH 1 to 7. The metal ions Mg 2+ , Ca 2+ , Ba 2+ , Mn 2+ , Al 3+ , Fe 2+ had a very clear function to accelerate milk coagulation whereas Na + and K + decelerated the activity slightly. The curd effect of the milk-clotting enzyme has primarily been studied.

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“…The extraction method obtains the lycopene through tomato, but it is a very expensive process because of being short of the rare sources containing high levels of this natural dye. The fermentation method has been widely used for the production of various valuable substances [10][11][12][13][14][15][16][17][18][19][20][21][22][23] and regarded as the efficient route to obtain lycopene. More than 100 study organizations focus on this area and more attention has been paid to this method.…”

Section: Introductionmentioning

confidence: 99%

Protoplast from β-carotene-producing fungus Blakeslea trispora: Preparation, regeneration and validation

Yuan

2008

Korean J. Chem. Eng.

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The effects of key parameters on the preparation and regeneration of protoplast from the β-carotene-producing fungus Blakeslea trispora were discussed in this paper, including the combination of various enzymes, mycelial age, digesting time and temperature, pH value, osmotic stabilizers, pretreatment, culture medium and culture method. Under the condition of mixed enzymes in osmotic stabilizer (0.6 M NaCl) combined with 2% lysozyme, 3% cellulase and 3% snailase, the highest protoplast yield, as high as 7.48×10 6 protoplasts/mL, was obtained when mycelial age was 60 h at pH 5.0-6.0 with digesting for 14-16 h at 28 o C. After purification of the obtained protoplasts, they were regenerated in PDA regenerative medium using bilayer plate culture method. To validate the usability of the protoplasts, a novel plasmid with green fluorescent protein (GFP) was used in transformation for easy visual observation. The results showed that the protoplasts prepared by the optimized method were active and applicable in further gene manipulation experiments.

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Determination of agitation and aeration conditions for scale-up of cellulolytic enzymes production by Trichoderma inhamatum KSJ1

Cited by 8 publications

References 9 publications

Development of grapevine somatic embryogenesis using an air-lift bioreactor as an efficient tool in the generation of transgenic plants

Development of grapevine somatic embryogenesis using an air-lift bioreactor as an efficient tool in the generation of transgenic plants

Purification and partial characterization of milk-clotting enzyme extracted from glutinous rice wine mash liquor

Protoplast from β-carotene-producing fungus Blakeslea trispora: Preparation, regeneration and validation

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