Determination of aflatoxin B1 concentration in poultry feed in the poultry farms of Sanandaj using ELISA method

Abstract

Background and Aim: Aflatoxin B 1 is a common contaminant of poultry feed and has the highest rate of acute and chronic toxicity among all mycotoxins. Over the past decade, studies have well established the negative effects of aflatoxin B 1 on the health of poultry. Therefore, it is necessary to determine the maximum concentration of aflatoxin B 1 in poultry feed. Materials and Methods: In this study, in order to determine the aflatoxin B 1 concentration in poultry feed, 45 samples were collected via a simple random method during autumn 2018 and winter 2019, and then the concentration of this contaminant was measured by the ELISA method. Results:The experimental results showed that the contamination range in the positive samples was between 6.44 and 18.34 µg/Kg. Moreover, the mean concentration of aflatoxin in contaminated samples was 10.5 µg/Kg. The concentration of contaminant in 46.66% of the samples exceeded the standard limit. Due to higher temperatures and suitable environmental conditions for the growth of fungi, the samples prepared in autumn were more contaminated than those prepared in winter. Conclusion:The level of aflatoxin B 1 contamination in poultry feed in Sanandaj is high. In order to prevent the entry of this toxin into the human food cycle regular survey and monitoring of poultry feed by health institutions are necessary.

Assessment of Poultry Feed Contamination Level by Aflatoxin B1: Quantification by Two Chromatographic Analysis Methods

“…AFB1 quantification by Enzyme-Linked Immunosorbent Assay (ELISA) on Iranian farms feed showed compliant results relatively to international standards (between 6.44 and 18.34 ppb). Samples collected in autumn were more contaminated than those sampled during winter [39]. This technique also provided compliant results over Indian manufactures poultry feed (18.7 ppb maximum level) [40].…”