Determination of aflatoxin-B<sub>1</sub>in poultry feed and its components employing enzyme-linked immunosorbent assay (ELISA)

Maqbool, Uzma; Ahmad, Maqbool; Anwar-ul-Haq,; Iqbal, M. Mohsin

doi:10.1080/02772240400007138

Cited by 14 publications

(7 citation statements)

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“…Corn is imported, bran and copra are produced in Guyana, while wheat middling is a product of the flour produced in Guyana from imported wheat. Both finished poultry feed and feed ingredients were found to be contaminated with aflatoxins in Pakistan [ 19 ]. In Venezuela, 17 of the 40 samples of poultry feed ingredients tested were contaminated with aflatoxins, with the highest concentration of aflatoxin found in corn flour [ 20 ].…”

Section: Resultsmentioning

confidence: 99%

A Limited Survey of Aflatoxins in Poultry Feed and Feed Ingredients in Guyana

Morrison

Ledoux

Chester

et al. 2017

Veterinary Sciences

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Abstract:A study was conducted to determine the presence of aflatoxins in finished poultry feed from manufacturing companies, feed ingredients, and poultry feed at the point of sale. Two collections were made. In the first collection, samples of the finished feed and feed ingredients were analyzed by high-performance liquid chromatography (HPLC). For the second collection, all samples were analyzed by ELISA while a subset was analyzed by HPLC. Of the 27 samples of finished feed, five samples had aflatoxin concentrations greater than the United States Food and Drug Administration (USFDA) and European Union Commission (EUC) maximum tolerable limit of 20 µg/kg, while for the feed ingredients, three of the 30 samples of feed ingredients exceeded the limit. Of the 93 samples of finished feed purchased from retailers, five samples had aflatoxin concentrations greater than the maximum tolerable limit. This survey indicates that most of the samples were below the maximum regulatory limit and maintained quality up to the point of sale for 2015 and 2016. However, given that some samples were above the limit, there is a need to monitor the production and marketing chain to ensure that the quality of the finished feed is not compromised.

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Section: Resultsmentioning

confidence: 99%

A Limited Survey of Aflatoxins in Poultry Feed and Feed Ingredients in Guyana

Morrison

Ledoux

Chester

et al. 2017

Veterinary Sciences

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“…Mycological analysis of the positive samples revealed 39 isolates of which only 1 ( A. parasiticus ) was reported as aflatoxigenic. Poultry feedstuffs from Faisalabad were also found contaminated with AFB 1 concentrations higher than 12.5 μg/kg when analyzed by ELISA (Maqbool and others ).…”

Section: Mycotoxin Contamination Of Food and Feed In Pakistanmentioning

confidence: 99%

Natural Occurrence of Mycotoxins in Food and Feed: Pakistan Perspective

Ashiq

2014

Comp Rev Food Sci Food Safe

105

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Fungi are commonly present in the environment and can grow under favorable conditions on an extensive variety of substrates. During harvesting, handling, storage, and distribution, agricultural commodities are subjected to infection by toxigenic molds, which may cause spoilage and produce toxic metabolites called mycotoxins. Fungal contamination of various food commodities with consequent exposure of the community to mycotoxins is a hazard that may exist depending on environmental factors, crop health, and soil conditions. Mycotoxins represent serious consequences due to substantial economic loss and risk to health. The environmental conditions of Pakistan with its mostly warm temperature are conducive to growth of toxigenic fungi resulting in mycotoxin production in different food items. Moreover, the poor conditions of storage and deficiency in regulatory measures in food quality control worsen the situation in the country. This review encompasses mycotoxin contamination of food and feed in Pakistan. High concentrations of mycotoxins are found in some commodities that are used on a daily basis in Pakistan, which may be a concern depending on dietary variety and health conditions of individuals in the population. Therefore, the mycotoxin contamination of foodstuff with exceeding levels represents a serious health hazard for the local population. There is a need to conduct more studies to analyze mycotoxin occurrence in all types of food commodities throughout the country. For consumer safety and the country's economy, the regulatory authorities should take into account this issue of contamination, and control strategies should be implemented and the quality control system of food improved.

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“…AFs are found as contaminants in various agricultural commodities such as cereals, tree nuts, groundnut, and cottonseed [ 2 ]. They are considered the most serious threat to public health due to their carcinogenic and hepatotoxic, teratogenic and mutagenic effect in human and animals [ 3 – 5 ].…”

Section: Introductionmentioning

confidence: 99%

Isolation of Aflatoxin B1 from Moldy Foods by Solid-Phase Extraction Combined with Bifunctional Ionic Liquid-Based Silicas

Fang

Tian

Yan

et al. 2018

Journal of Analytical Methods in Chemistry

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A solid-phase extraction method was developed by using new bifunctional ionic liquid-based silicas as sorbents to isolate aflatoxin B1 from moldy corn and peanut. Firstly, according to the adsorption efficiency, two sorbents imidazolium chloride-butylimidazolium chloride-based silica (Sil@BIm-Im) and imidazolium chloride-hexylimidazolium chloride-based silica (Sil@HIm-Im) were selected. The RSM was introduced to optimize adsorption conditions such as methanol/water ratio, time, and pH. Sil@HIm-Im, which had the highest adsorption efficiency, was used in SPE as a sorbent. After 2.0 mL of loading samples, washing solvents were optimized as 6.0 mL and 4.0 mL of water for corn and peanut, 2.0 mL of acetonitrile, and 3.0 mL of methanol. 3.0 mL of methanol/acetic acid (2.0% vol.) was investigated as an elution solvent. Finally, 0.009 μg/g and 0.023 μg/g of aflatoxin B1 were obtained in corn and peanut extract with recoveries of 80.0%–103.3% and RSDs of 2.37%–6.58%.

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Determination of aflatoxin-B₁in poultry feed and its components employing enzyme-linked immunosorbent assay (ELISA)

Cited by 14 publications

References 10 publications

A Limited Survey of Aflatoxins in Poultry Feed and Feed Ingredients in Guyana

A Limited Survey of Aflatoxins in Poultry Feed and Feed Ingredients in Guyana

Natural Occurrence of Mycotoxins in Food and Feed: Pakistan Perspective

Isolation of Aflatoxin B1 from Moldy Foods by Solid-Phase Extraction Combined with Bifunctional Ionic Liquid-Based Silicas

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Determination of aflatoxin-B1in poultry feed and its components employing enzyme-linked immunosorbent assay (ELISA)

Cited by 14 publications

References 10 publications

A Limited Survey of Aflatoxins in Poultry Feed and Feed Ingredients in Guyana

A Limited Survey of Aflatoxins in Poultry Feed and Feed Ingredients in Guyana

Natural Occurrence of Mycotoxins in Food and Feed: Pakistan Perspective

Isolation of Aflatoxin B1 from Moldy Foods by Solid-Phase Extraction Combined with Bifunctional Ionic Liquid-Based Silicas

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Determination of aflatoxin-B₁in poultry feed and its components employing enzyme-linked immunosorbent assay (ELISA)