Determination of Aflatoxin B 1 in Highly Contaminated Peanut Samples Using HPLC and ELISA

Validation of the procedure for the determination of aflatoxin B1 in animal liver using immunoaffinity columns and liquid chromatography with postcolumn derivatisation and fluorescence detection

“…Analytical methods based on immunoaffinity column clean-up and liquid chromatography represent the most recent and advanced tool for the determination of mycotoxins. Several articles reporting the use of these methods for determination of aflatoxins in feeds (Sharman & Gilbert, 1991) and different food matrices (Asis, Di Paola, & Aldao, 2002;Daradimos, Marcaki, & Koupparis, 2000;Patey, Sharman, & Gilbert, 1990;Stroka, Anklam, Jö rissen, & Gilbert, 2000, 2001 are available, but to our knowledge there was no report until now on the use of these specific methods for the determination of aflatoxins in food of animal origin, except aflatoxin M 1 in milk (Dragacci, Grosso, & Gilbert, 2001;Rodríguez Velasco, Calonge Delso, & Ordónez Escudero, 2003). In view of the information on the food of animal origin naturally contaminated with aflatoxins given by FAO (1998), it seems reasonable to provide a precise and reliable analytical method for the determination of aflatoxin B 1 in food of animal origin.…”

Rapid Simultaneous Determination of Eight Benzimidazoles in Animal Feed by LC–MS–MS

“…To determine benzimidazoles in biological samples, bioassay [3], TLC [4,5], ELISA [6,7], LC with UV or FLD [8][9][10][11][12][13], GC [14], GC-MS [15], and LC-MS-MS [16][17][18] methods have been developed. At present most of the methods for the determination of benzimidazole residues in biological matrices using LC with UV and/or fluorescence detection.…”

Influence of Soluble Feed Proteins and Clay Additive Charge Density on Aflatoxin Binding in Ingested Feeds

“…The modified procedure first equilibrates AfB1/corn meal/clay in water (more like the environment of ingested aflatoxins) prior to extraction with 60% methanol. This contrasts with the immediate 60% methanol extraction used by Asis et al (2002). The AfB1 adsorption data were fitted to a line using least squares linear regression and the slopes or adsorption coefficients (K d ) were calculated to compare relative adsorption capacities.…”

“…A modification of the Asis et al (2002) enzyme-linked immunoassay (ELISA) method was used for measuring aflatoxins. The modified method is thoroughly described in Jaynes et al (2007).…”

“…Aflatoxins are more soluble in methanol than in water. The 60% methanol extraction was modified from the Asis et al (2002) procedure to extract and measure aflatoxins in peanuts. The AOCS method for aflatoxins in corn, cottonseed, peanuts, and peanut butter similarly uses an 80% methanol/20% water extraction (AOCS, 1999b).…”