Determination of Adenosine Triphosphoric Acid by Its Effect on the Quenching of the Fluorescence of Europium(III) Diketonate in Micelles of Brij-35

Штыков, С. Н.; Smirnova, Tatiana; Bylinkin, Yu. G.

doi:10.1023/b:janc.0000026234.48343.ac

Cited by 14 publications

(8 citation statements)

References 14 publications

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“…In this way, ATP could be determined in fruit juices with a limit of detection of 0.08 μM. However, the choice of the chelate implies UV excitation (330 nm) …”

Section: Bioanalyses With Lanthanide Luminescent Probesmentioning

confidence: 99%

Lanthanide Luminescence for Biomedical Analyses and Imaging

2010

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Jean-Claude Bu ¨nzli is an active researcher in the field of co-ordination, supramolecular, and biological chemistry of the lanthanide ions. He earned a degree in chemical engineering in 1968 and a Ph.D. in 1971 from the E ´cole Polytechnique Fe ´de ´rale de Lausanne (EPFL). He spent two years at the University of British Columbia (Canada) and one year at the Swiss Federal Institute of Technology in Zu ¨rich before being appointed at the University of Lausanne in 1974 and at EPFL in 2001 as a full professor of inorganic chemistry.

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“…In this way, ATP could be determined in fruit juices with a limit of detection of 0.08 μM. However, the choice of the chelate implies UV excitation (330 nm) …”

Section: Bioanalyses With Lanthanide Luminescent Probesmentioning

confidence: 99%

Lanthanide Luminescence for Biomedical Analyses and Imaging

2010

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“…According to the 3s (standard deviation) rule, the detection limit was determined to be 0.14 nM. It is comparable with or better than some commonly used techniques, such as bioluminometric assays (with a detection limit of 200 nM), 38 uorometric assays (with a detection limit of 80 nM), 17 electrochemical assays (with a detection limit of 70 nM) 22 and enzyme biosensors (with a detection limit of 200 nM). 39 This Analyst Paper assay is convenient, quick and sensitive, and has a wide dynamic range, which makes it a useful approach to analyze the ATP.…”

Section: High Sensitivity For Atp Quantitationmentioning

confidence: 98%

“…15,16 Furthermore, the requirement of highly sensitive intensied cameras to achieve the sufficient sensitivity and resolution restricts its applications. [12][13][14] Fluorometric, [17][18][19] chemiluminescent 20,21 and electrochemical 22,23 detection has also been developed with the detection limits ranging from 10 to 1000 nM. Ellington et al recently used a deoxyribozyme ligase and rolling circle ampli-cation to detect ATP, 24 the lowest ATP detection concentration in their report was 1000 nM.…”

Section: Introductionmentioning

confidence: 99%

A novel sensitive and selective ligation-based ATP assay using a molecular beacon

Tang

Wang

et al. 2013

Analyst

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In this paper, a novel, facile fluorometric ATP assay with high sensitivity and excellent selectivity has been reported. This approach utilizes a molecular beacon, T4 DNA ligase and two short oligonucleotides. In the presence of ATP, the T4 DNA ligase catalyzes the ligation reaction and the ligation product restores the fluorescence of the molecular beacon. Owing to the high sensitivity of the molecular beacon and T4 DNA ligase's high substrate dependence, this novel ATP assay demonstrates exceptional selectivity and high sensitivity down to 0.14 nM in homogeneous solution. Cellular ATP concentrations in several cell lines have been determined by measuring the lysate sample containing 8.0 × 10(3) cells.

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“…Many Eu 3+ ‐complexes have been reported to respond to phosphate ( 2, 9–11 ) 38–40 or ATP ( 2, 9 , 12–14 ) 41–45 . As in the course of phosphatase reactions phosphate anions are released, EuTC (3:1) was used to monitor alkaline phosphatase (ALP) activity 46 .…”

Section: Metal Complexes As Probes For Nucleoside Phosphates and Inormentioning

confidence: 99%

“…Many Eu 3+ -complexes have been reported to respond to phosphate (TABLE 2, 9-11) [38][39][40] or ATP (TA-BLE 2, 9, 12-14). [41][42][43][44][45] As in the course of phosphatase reactions phosphate anions are released, EuTC (3:1) was used to monitor alkaline phosphatase (ALP) activity. 46 Using phenyl phosphate as substrate, a linear range of 1.25 to 15 mU mL −1 with a LOD of 2.6 mU mL −1 of ALP was obtained.…”

Section: Eu(iii)-complexesmentioning

confidence: 99%

Luminescent Lanthanide Complexes as Probes for the Determination of Enzyme Activities

Spangler

Schäerling

2008

Annals of the New York Academy of Sciences

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The determination of enzyme activities and the screening of enzyme regulators is a major task in clinical chemistry and the development of new drugs. A broad variety of enzymatic reactions is associated with the consumption or formation of small molecules like H(2)O(2), ATP, pyrophosphate, or phosphate. Luminescent lanthanide complexes can be applied to monitor these enzymatic conversions and therefore can serve as probes for the determination of enzyme activities. The utility of this concept will be demonstrated by means of some selected examples including europium and terbium complexes. Accordingly, this new approach could be already implemented for the determination of glucose oxidase, catalase, and peroxidase activity. In particular, enzymes that catalyze phosphorylation or dephosphorylation reactions came to the fore of interest because of their high relevance as drug targets. These include (protein) kinases, adenylyl cyclases, phosphodiesterases, phosphatases, and ATPases. The development and design of fluorescent lanthanide complexes should lead to probes with optimized selectivity and response times that can be applied for high-throughput screening of enzyme inhibitors and for real-time monitoring of enzyme kinetics. In contrast to other assays for enzyme activity determination, this method does not require the use of radioactively labelled substrates or the accomplishment of rather complex and expensive immunoassays.

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Determination of Adenosine Triphosphoric Acid by Its Effect on the Quenching of the Fluorescence of Europium(III) Diketonate in Micelles of Brij-35

Cited by 14 publications

References 14 publications

Lanthanide Luminescence for Biomedical Analyses and Imaging

Lanthanide Luminescence for Biomedical Analyses and Imaging

A novel sensitive and selective ligation-based ATP assay using a molecular beacon

Luminescent Lanthanide Complexes as Probes for the Determination of Enzyme Activities

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