Determination of Acyclovir in Blood Serum and Plasma by Micellar Liquid Chromatography with Fluorimetric Detection

Macka, Mirek; Borák, J.; Seménková, L.; Popl, M.; Mikeš, Vladimír

doi:10.1080/10826079308020992

Cited by 21 publications

(9 citation statements)

References 25 publications

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“…The results of the degradation study showed that the concentrations of ACV in the samples were lowered compared to the original concentrations. This degradation study was particularly important for the analysis of lipid based formulation, which was not performed previously (Basavaiah et al, 2003, Macka et al, 1993.…”

Section: Forced Degradation Studymentioning

confidence: 99%

“…ACV was previously analyzed with several analytical methods like High Performance Liquid Chromatography (HPLC) (Teshima et al, 2003, Basavaiah et al, 2003, micellar liquid chromatography (Peh and Yuen, 1997;Macka et al, 1993), gas chromatography (Leis et al, 2002, Rakestraw, 1993, capillary electrophoresis (Vo et al, 2002, Reichova et al, 2002, radioimmunoassay (Tadepalli and Quinn, 1996), and potentiometry (Abdel-Ghani et al, 2002). None of these methods were specially developed in lipid based formulations except biological fluids and few in Acyclovir dosage forms (suspension).…”

Section: Introductionmentioning

confidence: 99%

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Development and validation of a UPLC method for quantification of antiviral agent, Acyclovir in lipid-based formulations

Alamri

Kazi

Alanazi

2019

Arabian Journal of Chemistry

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Purpose: The objective of the current study is to evaluate the Ultra Performance Liquid Chromatography (UPLC) method for quantification of Acyclovir in lipid-based formulations.Method: A simple, rapid, reliable and precise reversed phase UPLC method has been developed and validated according to the regulatory guidelines, which composed of isocratic mobile phase; 0.25% formic acid (FA) in Milli-Q water with a flow rate of 0.5 ml/min, and column BEH C18 (2.1 · 50 mm, 1.7 lm). The detection was carried out at 254 nm.Results: The developed UPLC method was found to be rapid (1.2 min run time), selective with well resoluted Acyclovir peak (0.89 min) from different lipid matrices and sensitive (Limit of Detection (LOD) was 0.3 ppm and Lower Limit of Quantification (LLOQ) was 1 ppm). The accuracy and precision were determined and were perfectly matching with the standard FDA limits.Conclusion: The study showed that the proposed UPLC method can be used for the assessment of drug purity, stability, solubility and lipid-formulation release profile with no interference of excipients or related substances of active pharmaceutical ingredient. ª 2014 Production and hosting by Elsevier B.V. on behalf of King Saud University.

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Section: Forced Degradation Studymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Development and validation of a UPLC method for quantification of antiviral agent, Acyclovir in lipid-based formulations

Alamri

Kazi

Alanazi

2019

Arabian Journal of Chemistry

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“…The fluorescence (FL) of ACV in acidic media is made possible by the protonation of the molecule. Recently, a micelle-enhanced fluorimetric detection method coupled with HPLC was successfully applied for ACV determination in biological fluids (Macka et al 1993). In the cited report, a mixture of sodium dodecyl sulfate and cetyltrimethylammonium bromide as micellar media not only greatly enhanced the fluorescence of ACV, but also provided good separation of ACV from serum matrix components under the optimized mobile phase conditions.…”

Section: Acyclovirmentioning

confidence: 98%

Fluorimetric study of acyclovir in acidic micellar media

Zhang

Baeyens

Weken

et al. 1999

Biomed. Chromatogr.

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“…After switching on the potential (no injection was performed) the signal was acquired with a frequency of 20 data points per second for a total interval of 2 min. As described earlier [21], using digital data on a selected linear piece of baseline, linear regression and then statistical analysis of the residuals were performed and finally the noise was determined as five times the calculated standard deviation of the data points at the selected baseline interval. In our series of experiments two 0.2 min long baseline segments (from 1.1 to 1.3 min and from 1.6 to 1.8 min) were evaluated and the average of the two baseline noise values was then calculated (the relative difference of the two values was less than 11% with all measurements).…”

Section: Detector Noise Measurementsmentioning

confidence: 99%

Linearity evaluation in absorbance detection: The use of light‐emitting diodes for on‐capillary detection in capillary electrophoresis

1996

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A model which takes into account both stray light and polychromatic light was used to predict and evaluate linearity in on-capillary detection in capillary electrophoresis (CE). According to the model the stray light is the major factor which determines linearity under typical CE operating conditions. By calculating theoretical absorbance versus concentration plots, the influence of different levels of stray light and polychromatic light on linearity is demonstrated. Experimentally, six light-emitting diodes (LEDs) in the range from 563 to 654 nm were examined as light sources for on-capillary detection in CE. Fitting theoretical curves to measured linearity plots enabled determination of the values of both effective path length and stray light for a particular detection system. The detector linearity for the four LEDs was compared to mercury and tungsten lamps used with interference filters. For potassium permanganate as the test compound, the linear range for a 563 nm LED was two times greater than that for a mercury lamp operated at 546 nm. The relatively poor linearity of the mercury lamp detector is explained by its high level of stray light. The noise of the LED563-based detector was the same as for the mercury lamp, whereas the other LEDs of higher light intensity gave approximately half the noise of the mercury lamp. The lowest noise level of 3 x 10(-5) AU was obtained for the LED at 554 nm (determined at a detector time constant of 0.1 s).

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Determination of Acyclovir in Blood Serum and Plasma by Micellar Liquid Chromatography with Fluorimetric Detection

Cited by 21 publications

References 25 publications

Development and validation of a UPLC method for quantification of antiviral agent, Acyclovir in lipid-based formulations

Development and validation of a UPLC method for quantification of antiviral agent, Acyclovir in lipid-based formulations

Fluorimetric study of acyclovir in acidic micellar media

Linearity evaluation in absorbance detection: The use of light‐emitting diodes for on‐capillary detection in capillary electrophoresis

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