Determination of acute phase proteins after experimental Streptococcus iniae infection in tilapia (Oreochromis niloticus L.)

Güleç, Azime Küçükgül; Cengizler, İbrahim

doi:10.3906/vet-1011-2

Cited by 2 publications

(2 citation statements)

References 17 publications

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“…Our results confirm the role of CRP in mediating the complex inflammatory response, limiting the dispersal of infectious agents, inactivating proteases, killing microbes, repair of tissue damage, and restoring the healthy one. All this suggest CRP as a bioindicator in the pathogenesis of fish infectious diseases 52 . Similar to our findings, the level of serum CRPs increased significantly in common carp ( Cyprinus carpio ) following infection with A. hydrophila 53 and in Nile tilapia experimentally infected with Streptococcus inia at 7, 14, and 21-day post-infection 52 and in Pacu, Piaractus mesopotamicus experimentally challenged with A. hydrophila (1 × 10 8 ) at day seven post infection 54 .…”

Section: Discussionmentioning

confidence: 93%

Host–pathogen interaction unveiled by immune, oxidative stress, and cytokine expression analysis to experimental Saprolegnia parasitica infection in Nile tilapia

Gamal

Adawy²,

Zaki

et al. 2023

Sci Rep

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The present study evaluated the pathogenicity, immunological, and oxidant/antioxidant responses against Saprolegnia parasitica (S. parasitica) infection in Nile tilapia (Oreochromis niloticus). Three groups of Nile tilapia were assigned as the control group (no zoospores exposure). The other two groups were challenged by Saprolegnia zoospores; one was used for sampling, and the other for mortality monitoring. The study lasted 3 weeks and was sampled at three point times at 1, 2, and 3 weeks. Results showed that S. parasitica zoospores were pathogenic to Nile tilapia, causing a cumulative mortality rate of 86.6%. Immunoglobulin M and C- reactive protein (IgM and CRP) levels showed a similar trend being significantly (P < 0.05, P < 0.001) higher in the infected group at weeks 1, 2, and 3, respectively, compared to the control group. Oxidant and antioxidant parameters in gills revealed that Malondialdehyde (MDA) level was significantly higher in the infected group compared to the control group. While catalase, glutathione peroxidase, and superoxide dismutase (CAT, GSH, and SOD) levels were significantly decreased in the infected group compared to the control group. Compared to the control, the tumor necrosis factor-α (TNF-α) gene was firmly upregulated in gill tissue at all-time points, particularly at day 14 post-infection. Meanwhile, Interleukin 1-β (IL-1 β) gene was significantly upregulated only at days 7 and 14 post-infection compared to control. Histopathological examination revealed destructive and degenerative changes in both skin and gills of experimentally infected Nile tilapia. Our findings suggest that Nile tilapia-S. parasitica infection model was successful in better understanding of pathogenicity and host (fish)-pathogen (oomycete) interactions, where the induced oxidative stress and upregulation of particular immune biomarkers in response to S. parasitica infection may play a crucial role in fish defense against oomycetes in fish.

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Section: Discussionmentioning

confidence: 93%

Host–pathogen interaction unveiled by immune, oxidative stress, and cytokine expression analysis to experimental Saprolegnia parasitica infection in Nile tilapia

Gamal

Adawy²,

Zaki

et al. 2023

Sci Rep

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“…The formation and function of APP may differ depending on the animal species. In fish such as in mammals, hepatocytes are the prime source of APPs (Güleç and Cengizler, 2012). In fish, concentrations of petraxins, to which CRP belongs, following an acute phase response rise slightly or decrease (Ellis, 2001).…”

Section: Introductionmentioning

confidence: 99%

ТHE SIGNIFICANCE OF CERULOPLASMIN AND C-REACTIVE PROTEIN AS POSSIBLE MARKERS FOR THE DIAGNOSIS OF MYXOBOLOUS INFECTION CAUSED BY Myxobolus cerebralis IN RAINBOW TROUT (Oncorhynchus mykiss)

ZAPRYANOVA,

URKU,

NIKOLOV

et al. 2023

VJRS

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Myxobolus cerebralis is the etiological agent of one of the most devastating diseases (Whirling disease) in salmonids. It belongs to subphylum Myxozoa which possesses the highest susceptibility to the rainbow trout (Oncorhynchus mykiss). Acute phase proteins as an essential component of normal homeostasis are well defined and are presently used as a diagnostic and prognostic marker in human and animal pathology for evaluation of infection and inflammation. In piscine medicine the importance and the function of acute phase proteins still has not been fully determined. Furthermore, parasitic infections are more difficult to diagnose than bacterial and viral infections due to the lack of specific diagnostic tools therefore the change in the levels of some acute phase proteins could possibly be used as an alternative marker for myxobolosis. Further research is still needed to develop an understanding of the diagnostic significance of acute phase proteins in fish and the differentiation of their values in different types of disorders. The present study evaluated the modulation of ceruloplasmin and C-reactive protein following inflammatory stimulus by natural infection of Myxobolus cerebralis in rainbow trout (Oncorhynchus mykiss). In the research, biochemical tests and enzyme-linked immunosorbent assay were carried out. The test material was a diseased rainbow trout with a mortality rate of 45%. In the future, acute phase proteins have a real potential to become widely used as diagnostic tools in piscine medicine, as the evaluation of their levels can help better diagnosis and treatment, especially when it occurs during illness. Measurement of C-reactive protein concentration in trout may be used useful as a bioindicator of the health condition of this species. Further research is still needed to develop an understanding of the diagnostic significance of acute phase proteins in fish and the differentiation of their values in different types of disorders.

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Determination of acute phase proteins after experimental Streptococcus iniae infection in tilapia (Oreochromis niloticus L.)

Cited by 2 publications

References 17 publications

Host–pathogen interaction unveiled by immune, oxidative stress, and cytokine expression analysis to experimental Saprolegnia parasitica infection in Nile tilapia

Host–pathogen interaction unveiled by immune, oxidative stress, and cytokine expression analysis to experimental Saprolegnia parasitica infection in Nile tilapia

ТHE SIGNIFICANCE OF CERULOPLASMIN AND C-REACTIVE PROTEIN AS POSSIBLE MARKERS FOR THE DIAGNOSIS OF MYXOBOLOUS INFECTION CAUSED BY Myxobolus cerebralis IN RAINBOW TROUT (Oncorhynchus mykiss)

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