Determination of acetylsalicylic acid and salicylic acid in skin and plasma by high-performance liquid chromatography

Pirola, Romano C.; Bareggi, Silvio R.; Benedittis, Giuseppe De

doi:10.1016/s0378-4347(97)00539-2

Cited by 61 publications

(38 citation statements)

References 7 publications

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“…Blood samples were centrifuged at 4,200 ϫ g for 10 min at 4°C. Specimens were subsequently stored at Ϫ20°C and assayed by HPLC (Prostar 330, Varian) according to a published method (24). The celecoxib concentrations were determined by using a HPLC assay with fluorescence detection for human plasma as described (25).…”

Section: Methodsmentioning

confidence: 99%

Interaction of a selective cyclooxygenase-2 inhibitor with aspirin and NO-releasing aspirin in the human gastric mucosa

Fiorucci

Santucci

Wallace

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

115

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In addition to inhibiting cyclooxygenase (COX)-1-derived prostanoid biosynthesis, aspirin acetylates COX-2, enabling the conversion of arachidonic acid to 15(R)-epi lipoxin A4, or aspirin-triggered lipoxin (ATL). Selective COX-2 inhibitors block ATL formation and exacerbate mucosal injury in rats treated with aspirin. In the present study, we have examined whether inhibition of COX-2 activity in healthy volunteers taking aspirin exacerbates gastric mucosal injury and if such an effect would be prevented by NCX-4016, a NO-releasing derivative of aspirin. Thirty-two volunteers were randomized to receive 2 wk of treatment with NCX-4016 (800 mg twice a day) or aspirin (100 mg once a day) alone or in combination with 200 mg of celecoxib twice a day. Mucosal damage was assessed by endoscopy. The mean mucosal injury score was 5.8 ؎ 1.8 in subjects treated with aspirin and 2.4 ؎ 0.7 (P < 0.01 vs. aspirin) in subjects treated with NCX-4016. Administration of celecoxib increased the injury score in volunteers treated with aspirin (9.9 ؎ 1.9) but not in subjects taking NCX-4016 (1.5 ؎ 0.8). Aspirin and NCX-4016 caused a comparable suppression of serum thromboxane B 2 levels and increased urinary excretion of ATL. Celecoxib inhibited endotoxin-induced prostaglandin E 2 generation in whole blood by Ϸ80% and abolished ATL formation.

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Section: Methodsmentioning

confidence: 99%

Interaction of a selective cyclooxygenase-2 inhibitor with aspirin and NO-releasing aspirin in the human gastric mucosa

Fiorucci

Santucci

Wallace

et al. 2003

Proc. Natl. Acad. Sci. U.S.A.

115

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“…SA is further metabolized to gentisic acid, salicyluric acid and other conjugates (Mays et al, 1984). A number of methods have been reported for the analysis of ASA and its metabolites in plasma samples, including HPLC (Peng et al, 1978;Buskin et al, 1982;Bakar and Niazi, 1983;Mays et al, 1984;O'Kruk et al, 1984;Shen et al, 1990;Kees et al, 1996;McMahon and Kelly, 1998;Pirola et al, 1998 and references therein), GC (Walter et al, 1974;Rance et al, 1975) and capillary electrophoresis (Hansen et al, 1998). The method using on-line solid-phase extraction (SPE) by a methylcellulose-immobilized-strong anion-exchanger (MC-SAX) in a column-switching HPLC system achieved the highest sensitivity among the assay methods previously reported.…”

Section: Introductionmentioning

confidence: 99%

Determination of acetylsalicylic acid and its major metabolite, salicylic acid, in human plasma using liquid chromatography–tandem mass spectrometry: application to pharmacokinetic study of Astrix^® in Korean healthy volunteers

Bae

Seo

Jung

et al. 2008

Biomedical Chromatography

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The first liquid chromatography-tandem mass spectrometry (LC/MS/MS) method for determination of acetylsalicylic acid (aspirin, ASA) and one of its major metabolites, salicylic acid (SA), in human plasma using simvastatin as an internal standard has been developed and validated. For ASA analysis, a plasma sample containing potassium fluoride was extracted using a mixture of ethyl acetate and diethyl ether in the presence of 0.5% formic acid. SA, a major metabolite of ASA, was extracted from plasma using protein precipitation with acetonitrile. The compounds were separated on a reversed-phase column with an isocratic mobile phase consisting of acetonitrile and water containing 0.1% formic acid (8:2, v/v). The ion transitions recorded in multiple reaction monitoring mode were m/z 179 --> 137, 137 --> 93 and 435 --> 319 for ASA, SA and IS, respectively. The coefficient of variation of the assay precision was less than 9.3%, and the accuracy exceeded 86.5%. The lower limits of quantification for ASA and SA were 5 and 50 ng/mL, respectively. The developed assay method was successfully applied for the evaluation of pharmacokinetics of ASA and SA after single oral administration of Astrix (entero-coated pellet, 100 mg of aspirin) to 10 Korean healthy male volunteers.

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“…Many determination methods of ASA for several matrices such as tablets (Gandhimathi et al, 2003;Franeta et al, 2002), plasma (Abu-Qare andAbou-Donia, 2001;Tagliaro et al, 1997;Kees et al, 1996;Pirola et al, 1998), urine (Abu-Qare andAbouDonia, 2001), stratum corneum (Pirola et al, 1998) and transdermal perfusate (McMahon et al, 1998) have been reported. In these reports, wavelengths for measuring absorbance ranged from 207 to 280 nm, and the sensitivities were at sub-microgram/mL levels.…”

Section: Introductionmentioning

confidence: 99%

“…For the pretreatment of ASA in plasma samples, deproteinization with perchloric acid (Tagliaro et al, 1997;Kees et al, 1996) and solid-phase extraction (SPE) with a C 18 cartridge (Abu-Qare and Abou-Donia, 222 Y. Ohwaki et al SHORT COMMUNICATION 2001;Pirola et al, 1998) were used. However, few methods have been applied to patients' samples (Pirola et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Semi‐micro column high‐performance liquid chromatography with UV detection for quantification of aspirin and salicylic acid and its application to patients' sera administered with low‐dose enteric‐coated aspirin

Ohwaki¹,

Yamane²,

Ishimatsu³

et al. 2007

Biomedical Chromatography

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A simultaneous determination of aspirin (ASA) and its metabolite, salicylic acid (SA), in human serum by a semi-micro column HPLC-UV was developed. A relatively small size of serum sample (100 microL) containing ASA and SA was cleaned up by a simple solid phase extraction. A good separation of ASA and SA could be achieved within 25 min using a semi-micro ODS column with an eluent of MeOH/0.7 mm phosphoric acid solution (pH 2.5) = 50:50 (v/v). The calibration curves for ASA and SA showed good linearity (r = 0.999) with the detection limits 114 and 38 ng/mL at a signal-to-noise ratio of 3, respectively. ASA and SA in patients' sera administered with low-dose enteric-coated aspirin were determined, and the concentration ranges obtained for ASA and SA were 1.2-2.2 and 0.5-57.3 microg/mL, respectively.

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Determination of acetylsalicylic acid and salicylic acid in skin and plasma by high-performance liquid chromatography

Cited by 61 publications

References 7 publications

Interaction of a selective cyclooxygenase-2 inhibitor with aspirin and NO-releasing aspirin in the human gastric mucosa

Interaction of a selective cyclooxygenase-2 inhibitor with aspirin and NO-releasing aspirin in the human gastric mucosa

Determination of acetylsalicylic acid and its major metabolite, salicylic acid, in human plasma using liquid chromatography–tandem mass spectrometry: application to pharmacokinetic study of Astrix^® in Korean healthy volunteers

Semi‐micro column high‐performance liquid chromatography with UV detection for quantification of aspirin and salicylic acid and its application to patients' sera administered with low‐dose enteric‐coated aspirin

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Determination of acetylsalicylic acid and salicylic acid in skin and plasma by high-performance liquid chromatography

Cited by 61 publications

References 7 publications

Interaction of a selective cyclooxygenase-2 inhibitor with aspirin and NO-releasing aspirin in the human gastric mucosa

Interaction of a selective cyclooxygenase-2 inhibitor with aspirin and NO-releasing aspirin in the human gastric mucosa

Determination of acetylsalicylic acid and its major metabolite, salicylic acid, in human plasma using liquid chromatography–tandem mass spectrometry: application to pharmacokinetic study of Astrix® in Korean healthy volunteers

Semi‐micro column high‐performance liquid chromatography with UV detection for quantification of aspirin and salicylic acid and its application to patients' sera administered with low‐dose enteric‐coated aspirin

Contact Info

Product

Resources

About

Determination of acetylsalicylic acid and its major metabolite, salicylic acid, in human plasma using liquid chromatography–tandem mass spectrometry: application to pharmacokinetic study of Astrix^® in Korean healthy volunteers