Determination of acetylcholinesterase and α-glucosidase inhibition by electrophoretically-mediated microanalysis and phenolic profile by HPLC-ESI-MS/MS of fruit juices from Brazilian Myrtaceae Plinia cauliflora (Mart.) Kausel and Eugenia uniflora L.

Abstract

Alzheimer's disease and diabetes mellitus are contemporary diseases of great concern. Phenolic compounds are linked to several health benefits and could lead to novel strategies to combat these ailments. The objective of this study was to evaluate by electrophoretically-mediated microanalysis the potential inhibitory activity of the fruit juices from Plinia cauliflora ("jaboticaba") and Eugenia uniflora ("pitanga") toward acetylcholinesterase (AChE) and α-glucosidase, target enzymes in strategies for the treatment of these diseases. The phenolic profiles of the samples were also investigated. Jaboticaba and pitanga juices

Quantitative analysis of phenolic compounds in crude extracts of Myrcia splendens leaves by HPLC-ESI-MS/MS

“…Analysis of phenolic compounds was performed according Siebert et al (2019). This analysis is conducted using Agilent ® 1,200 chromatograph, with a Phenomenex ® Synergi 4μ Polar-RP 80A column (150 mm x 2 mm i.d., particle size of 4 μm) at 30 o C. The liquid chromatograph was coupled to a mass spectrometry system consisting of a hybrid triple quadrupole/linear ion trap mass spectrometer Qtrap ® 3,200 (Applied Biosystems/ MDS SCIEX, USA) with TurboIonSpray ® as the ionization source, in negative ionization mode, with the following source parameters: ion spray interface at 400 o C; ion spray voltage of 4,500 V; curtain gas, 10 psi; nebulizer gas, 45 psi; auxiliary gas, 45 psi; and collision gas, medium.…”

Evaluation of the Health-Promoting Properties of Selected Fruits

“…Eugenia uniflora L. fruits, popularly known as “Suriname cherry” or “pitanga” is an exotic fruit native from Brazil [ 16 ], but widely available in Madeira Island. Pitanga is appreciated by consumers for its softness, aromatic and bittersweet flavour, and presents a low lipid and caloric content and high amounts of phenolic compounds [ 17 , 18 ], carotenoids and anthocyanins [ 19 ]. Several ethnomedical uses of E. uniflora have been documented, especially those related to leaves and oils extracts [ 20 ], which present in vitro antiproliferative potential [ 21 ].…”

Bioactive Compounds of Fruit Parts of Three Eugenia uniflora Biotypes in Four Ripening Stages

“…Several biological activities have been reported for E. uniflora fruit extract, such as prevention of hyperglycemia and hypertriglyceridemia caused by dexamethasone-induced insulin resistance in an animal model and prevented the formation of thiobarbituric acidreactive substances and reactive oxygen species production in the liver of rats, [3] as well as inhibition by the fruit juice of acetylcholinesterase and αglucosidase enzymes, target enzymes for the treatment of Alzheimer's disease and diabetes mellitus. [4] Antioxidant activity of ethanolic peel/pulp extract has also been proven in vivo using the nematode Caenorhabditis elegans as a model for studying ageing mechanisms. [5] E. uniflora seed extracts showed anti-oxidant (reducing power, DPPH and ABTS assays), antibacterial (S. aureus, E. coli, P. aeruginosa and B. subtilis) activities, as well as high arginase from Leishmania enzyme inhibition, which can contribute to the control of Leishmaniosis disease.…”

“…[6] These studies have related such biological activities to phenolic compound content in the fruit. [3][4][5][6] While hydrolysable tannins and glycosylated flavonoids have been isolated and identified from E. uniflora leaves, [7,8] no isolation and identification of pure compounds from the fruits has yet been carried out. To date, some of pitanga's phenolic compounds have had their structures entirely or partially suggested by HPLC/MS.…”

“…E. uniflora seed extracts showed antioxidant (reducing power, DPPH and ABTS assays), antibacterial ( S. aureus , E. coli , P. aeruginosa and B. subtilis ) activities, as well as high arginase from Leishmania enzyme inhibition, which can contribute to the control of Leishmaniosis disease [6] . These studies have related such biological activities to phenolic compound content in the fruit [3–6] …”