Determination of acetaminophen and its main metabolites in urine by capillary electrophoresis hyphenated to mass spectrometry

Lecoeur, Marie; Rabenirina, Guy; Schifano, Nadège; Odou, Pascal; Ethgen, Sabine; Lebuffe, Gilles; Foulon, Catherine

doi:10.1016/j.talanta.2019.07.003

Cited by 38 publications

(11 citation statements)

References 29 publications

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Section: Figure 1 Schematic Representation Of the Principal Biochemic...mentioning

confidence: 99%

“…CYGB deficiency in HSCs can regulate hepatic O2 levels and can alleviate the acute liver injury induced by APAP, by decelerating CYP2E1 bioactivation in hepatocytes [52]. Additionally, HSCs produce several pro-inflammatory cytokines that may aggravate the drug-induced inflammation [53]. Primary bile acids cholic and chenodeoxycholic acid are synthesized by hepatocytes, conjugated with taurine, glycine, and sulphate, and excreted into bile, reaching the intestine.…”

Section: Figure 2 Schematic Representation Of the Biochemical Mechani...mentioning

confidence: 99%

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The Potential Role of Metabolomics in Drug-Induced Liver Injury (DILI) Assessment

2022

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Drug-induced liver injury (DILI) is one of the most frequent adverse clinical reactions and a relevant cause of morbidity and mortality. Hepatotoxicity is among the major reasons for drug withdrawal during post-market and late development stages, representing a major concern to the pharmaceutical industry. The current biochemical parameters for the detection of DILI are based on enzymes (alanine aminotransferase (ALT), aspartate aminotransferase (AST), gamma-glutamyl transpeptidase (GGT), alkaline phosphatase (ALP)) and bilirubin serum levels that are not specific of DILI and therefore there is an increasing interest on novel, specific, DILI biomarkers discovery. Metabolomics has emerged as a tool with a great potential for biomarker discovery, especially in disease diagnosis, and assessment of drug toxicity or efficacy. This review summarizes the multistep approaches in DILI biomarker research and discovery based on metabolomics and the principal outcomes from the research performed in this field. For that purpose, we have reviewed the recent scientific literature from PubMed, Web of Science, EMBASE, and PubTator using the terms “metabolomics”, “DILI”, and “humans”. Despite the undoubted contribution of metabolomics to our understanding of the underlying mechanisms of DILI and the identification of promising novel metabolite biomarkers, there are still some inconsistencies and limitations that hinder the translation of these research findings into general clinical practice, probably due to the variability of the methods used as well to the different mechanisms elicited by the DILI causing agent.

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Section: Figure 1 Schematic Representation Of the Principal Biochemic...mentioning

confidence: 99%

Section: Figure 2 Schematic Representation Of the Biochemical Mechani...mentioning

confidence: 99%

The Potential Role of Metabolomics in Drug-Induced Liver Injury (DILI) Assessment

2022

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“…Therefore, developing a simple, fast response, economical, sensitive, accurate, and reliable detection method for the assessment of PA is highly demanded in the medical field. There are lots of methods like capillary electrophoresis 11 , titrimetry 12 , SEC, LC–MS, HPLC 13 – 15 , chemiluminescence 16 , spectrofluorimetric 17 , and spectrophotometry 18 – 20 which have been availed for the assessment of PA. Among all these methods, the electrochemical method stands out with its simplicity, sensitivity, selectivity, modest and fast response.…”

Section: Introductionmentioning

confidence: 99%

Poly (Orange CD) sensor for paracetamol in presence of folic acid and dopamine

Sukanya

Swamy

Shashikumara

et al. 2021

Sci Rep

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In the present work, Orange CD was chosen as an intriguing modifier for the electropolymerization on the surface of CPE by the CV technique. A novel, sensitive, and cost-effective poly (Orange CD) MCPE (PoOCD/MCPE) sensor was utilized for the selective detection of paracetamol (PA) in 0.2 M phosphate buffer solution (PBS) of pH 7.4. The oxidation peak current of PA was vastly enhanced at the sensor. The scan rate study is suggested that electro-oxidation of PA was adsorption-controlled. The pH study testifies the redox pathways transport with the same quantity of electrons and protons. The detection limit of PA is found to be 2.64 µM. DPV results show that substantial peak separation between PA, folic acid (FA), and dopamine (DA) could be facilitating their individual and simultaneous determination on the sensor. The decorated sensor demonstrates high sensitivity, stability, reproducibility, repeatability and has been successfully exploited for the detection of PA in a tablet with promising results.

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“…Scheme 1. Structure of paracetamol Numerous analytical techniques including fluorescence (Moreira et al, 2005), high performance liquid chromatography (Shinde et al, 1995;Goyal and Jain, 2007), spectrophotometry (Baptistao et al, 2011), capillary electrophoresis hyphenated to mass spectrometry (Lecoeur et al, 2019), and FTIR and Raman spectrometry (Al-Zoubi et al, 2002) are among the techniques used for the determination of PA in real samples. The aforementioned methods are expensive, time consuming and involve tedious sample pre-treatment procedure, and they are less sensitive and selective making them inappropriate for monotonous analysis (Li et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Differential pulse stripping voltammetric determination of paracetamol in pharmaceutical tablet samples using murexide modified carbon paste electrode

Yohannes

Erba

2021

Eth J Sci & Technol

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As an acetyl aniline drug, paracetamol (PA) has antipyretic and analgesic functions, which is suitable for the remedy of fever, headache and joint ache. The murexide modified carbon paste electrode was characterized by cyclic voltammetry and electrochemical impedance spectroscopy and compared with bare carbon paste electrode. In this study, murexide-carbon paste electrode (MXCPE) was described for the differential pulse stripping voltammetric determination of paracetamol in pharmaceutical tablet sample. The MXCPE exhibited a better electrocatalytical behavior for the oxidation of PA as evidenced by nearly two folds of current enhancement and a shift of the onset potential by 65 mV in comparison with a bare carbon paste electrode. Differential pulse stripping voltammetry peak currents of PA increased linearly with their concentrations in the ranges of 5-1000 mM with a detection limit of 0.09 mM (S/N = 3). The determination of the analyte in pharmaceutical samples was found in the range 97.6-106.0% of the theoretical values and a recovery result between 94.6 and 96.8% was obtained. For selective determination of PA in the presence of AA was successfully performed.

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Determination of acetaminophen and its main metabolites in urine by capillary electrophoresis hyphenated to mass spectrometry

Cited by 38 publications

References 29 publications

The Potential Role of Metabolomics in Drug-Induced Liver Injury (DILI) Assessment

The Potential Role of Metabolomics in Drug-Induced Liver Injury (DILI) Assessment

Poly (Orange CD) sensor for paracetamol in presence of folic acid and dopamine

Differential pulse stripping voltammetric determination of paracetamol in pharmaceutical tablet samples using murexide modified carbon paste electrode

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