Determination of a setup correction function to obtain adsorption kinetic data at stagnation point flow conditions

Mora, María F.; Nejadnik, M. Reza; Baylon-Cardiel, Javier L.; Giacomelli, Carla E.; Garcı́a, Carlos D.

doi:10.1016/j.jcis.2010.02.019

Cited by 24 publications

(32 citation statements)

References 72 publications

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“…63 In agreement with previous experiments performed under similar conditions, 55,59,60,64 adsorbed proteins were represented by an additional layer (described with an additional Cauchy function) where A = 1.465, B = 0.01, and C = 0. These parameters yielded index of refraction values ranging from 1.527 to 1.477, which are consistent with previously reported values for other adsorbed proteins.…”

Section: Methodssupporting

confidence: 88%

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

et al. 2011

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This paper describes a simple and inexpensive procedure to produce thin-films of poly(dimethylsiloxane). Such films were characterized by a variety of techniques (ellipsometry, nuclear magnetic resonance, atomic force microscopy, and goniometry) and used to investigate the adsorption kinetics of three model proteins (fibrinogen, collagen type-I, and bovine serum albumin) under different conditions. The information collected from the protein adsorption studies was then used to investigate the adhesion of human dermal microvascular endothelial cells. The results of these studies suggest that these films can be used to model the surface properties of microdevices fabricated with commercial PDMS. Moreover, the paper provides guidelines to efficiently attach cells in BioMEMS devices.

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Section: Methodssupporting

confidence: 88%

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

et al. 2011

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“…In agreement with previous reports, MSE < 15 were considered acceptable. [16, 17] The ellipsometric measurements were interpreted using an optical model which describes the microstructure of the system under study in terms of the refractive index ( n ), extinction coefficient ( k ), and thickness ( d ). Therefore, the ellipsometric model used in this article to describe the experimental data was previously developed in our lab and presented in preceding papers.…”

Section: Experimental Designmentioning

confidence: 99%

“…Dynamic adsorption experiments were performed in a modified electrochemical cell[17] (J.A. Woollam Co.; Lincoln, NE) mounted directly on the vertical base of the ellipsometer, with an incident angle of 70°.…”

Section: Experimental Designmentioning

confidence: 99%

Adsorption and catalytic activity of glucose oxidase accumulated on OTCE upon the application of external potential

Benavidez¹,

Torrente²,

Marucho³

et al. 2014

Journal of Colloid and Interface Science

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This article describes the adsorption of glucose oxidase (GOx) onto optically transparent carbon electrodes (OTCE) under the effect of applied potential and the analysis of the enzymatic activity of the resulting GOx/OTCE substrates. In order to avoid electrochemical interferences with the enzyme redox center, control electrochemical experiments were performed using flavin adenine dinucleotide (FAD) and GOx/OTCE substrates. Then, the enzyme adsorption experiments were carried out as a function of the potential applied (ranged from the open circuit potential to +950 mV), the pH solution, the concentration of enzyme, and the ionic strength on the environment. The experimental results demonstrated that an increase in the adsorbed amount of GOx on the OTCE can be achieved when the potential was applied. Although the increase in the adsorbed amount was examined as a function of the potential, a maximum enzymatic activity was observed in the GOx/OTCE substrate achieved at +800 mV. These experiments suggest that although an increase in the amount of enzyme adsorbed can be obtained by the application of an external potential to the electrode, the magnitude of such potential can produce detrimental effects in the conformation of the adsorbed protein and should be carefully considered. As such, the article describes a simple and rational approach to increase the amount of enzyme adsorbed on a surface and can be applied to improve the sensitivity of a variety of biosensors.

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“…A. Woollam Co., Lincoln, NE) using the mean square error (MSE) to quantify the difference between the experimental and model-generated data. The ellipsometry experiments herein discussed were performed in either air or an ad-hoc cell [34] by performing spectroscopic scans in the 300 to 800 nm range (with 10 nm steps) using air or the corresponding aqueous buffer as the ambient medium.…”

Section: Methodsmentioning

confidence: 99%

Immobilization of glucose oxidase to nanostructured films of polystyrene-block-poly(2-vinylpyridine)

Bhakta¹,

Benavidez²,

Garcı́a³

2014

Journal of Colloid and Interface Science

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A critical step for the development of biosensors is the immobilization of the biorecognition element to the surface of a substrate. Among other materials that can be used as substrates, block copolymers have the untapped potential to provide significant advantages for the immobilization of proteins. To explore such possibility, this manuscript describes the fabrication and characterization of thin-films of polystyrene-block-poly(2-vinylpyridine) (PS-b-P2VP). These films were then used to investigate the immobilization of glucose oxidase, a model enzyme for the development of biosensors. According to the results presented, the nanoporous films can provide significant increases in surface area of the substrate and the immobilization of larger amounts of active enzyme. The characterization of the substrate-enzyme interface discussed in the manuscript aims to provide critical information about relationship between the surface (material, geometry, and density of pores), the protein structure, and the immobilization conditions (pH, ionic strength, and protein concentration) required to improve the catalytic activity and stability of the enzymes. A maximum normalized activity of 3300 ± 700 U m−2 was achieved for the nanoporous film of PS-b-P2VP.

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Determination of a setup correction function to obtain adsorption kinetic data at stagnation point flow conditions

Cited by 24 publications

References 72 publications

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

Adsorption of proteins to thin-films of PDMS and its effect on the adhesion of human endothelial cells

Adsorption and catalytic activity of glucose oxidase accumulated on OTCE upon the application of external potential

Immobilization of glucose oxidase to nanostructured films of polystyrene-block-poly(2-vinylpyridine)

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