Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography–mass spectrometry

Noh, Keumhan; Kim, Seo Young; Kam, Yoo Lim; Choo, Hea-Young Park; Lee, Hwa Jeong; Kang, Wonku

doi:10.1016/j.jpba.2010.10.007

Cited by 7 publications

(2 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…From this point of view, the detection of anesthetic residues in uterus and umbilical cord is beneficial for the diagnosis of drugs’ placental transportation. In recent years, the rapid development of liquid chromatography (LC) and gas chromatography (GC) has enabled huge progress in the pharmacokinetics study of anesthetics due to the high sensitivity of these techniques [3, 7, 12, 15]. However, the application of LC or GC in the placental transfer of Telazol was barely reported.…”

mentioning

confidence: 99%

Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study

Zhong

et al. 2017

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

Anesthetics utilized for the immobilization of pregnant mammals are prone to crossing the placental barrier and cause adverse effects to the fetuses. In this study, we develop a facile method employing high performance liquid chromatography (HPLC) for the study of Telazol crossing the placental barrier of pregnant pigs. The method mainly relies on the efficient extraction strategy that includes the mobile phase composed of 10 mM ammonium acetate aqueous solution-acetonitrile (1:4, v/v). When the injected dose of Telazol is 10 mg/kg (5 mg/kg of each constituent drug, zolazepem and tiletamine), zolazepam can cross the placental barrier as it is detected in both uterus and umbilical cord with approximately the same content. Conversely, tiletamine is detected in neither uterus nor umbilical cord, indicating the absence of placental transfer of tiletamine. The different absorption rates of the two dosage-equal compounds by pigs are found to be the main cause of their different abilities to cross the placental barrier.

show abstract

mentioning

confidence: 99%

Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study

Zhong

et al. 2017

The Journal of Veterinary Medical Science

View full text Add to dashboard Cite

show abstract

“…At the beginning of this study, we evaluated the linear relationship between the concentrations for calibration and the corresponding peak areas using various amounts of clean‐up solvent. Although the 3‐fold solvent ratio previously used (Noh et al , , ) provided picogram levels for the LOQ of each drug, the response was saturated from 100 and 500 ng/mL of zolazepam and tiletamine, respectively.…”

Section: Resultsmentioning

confidence: 99%

Simultaneous determination of zolazepam and tiletamine in dog plasma by liquid chromatography coupled to a tandem mass spectrometry

Noh

Kim

Ahn

et al. 2012

Biomedical Chromatography

Self Cite

View full text Add to dashboard Cite

A mixture of tiletamine, a dissociative anesthetic, and zolazepam, a minor tranquilizer, has been widely used as an anesthetic or an immobilizing agent in a variety of animal species. However, interestingly, their pharmacokinetic behaviors have been published only in polar bears and pigs. In this study, we introduce a sensitive liquid chromatography/tandem mass spectrometry (LC/MS/MS) method for determining the two drugs in dog plasma. After simple protein precipitation with acetonitrile including midazolam (internal standard), the analytes were chromatographed on a reversed-phase column with a mobile phase of 10 m m ammonium acetate aqueous solution and acetonitrile (1:4, v/v). The accuracy and precision of the assay were in accordance with FDA regulations for the validation of bioanalytical methods. This method was used to measure the concentrations of zolazepam and tiletamine in plasma after a single intramuscular 10 mg dose of each in beagle dogs.

show abstract

Quantitative determination of daumone in rat plasma by liquid chromatography–mass spectrometry

Noh

Park

et al. 2011

Journal of Pharmaceutical and Biomedical Analysis

View full text Add to dashboard Cite

Determination of a novel low-voltage-activated calcium channel blocker (HYP-10) in rat plasma by liquid chromatography–mass spectrometry

Cited by 7 publications

References 6 publications

Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study

Validation of an HPLC assay for determination of Telazol in pregnant pigs: application to placental transfer study

Simultaneous determination of zolazepam and tiletamine in dog plasma by liquid chromatography coupled to a tandem mass spectrometry

Quantitative determination of daumone in rat plasma by liquid chromatography–mass spectrometry

Contact Info

Product

Resources

About