Determination of a new oral iron chelator, ICL670, and its iron complex in plasma by high-performance liquid chromatography and ultraviolet detection

A new HPLC UV validated method for therapeutic monitoring of deferasirox in thalassaemic patients

“…Characterized by a wider range of concentrations and by an easier procedure than methodology presented by Rouan [19], our technique allows DFX quantification without MS facilities, not always present in standard laboratory [22]. Based on recent findings, analysis of glucuronide, has been avoided, considering that metabolite has no actual clinical use in patients and do not interferes with DFX in the chromatographic run [22].…”

“…In recent years, numerous papers have reported the use of high throughput bioanalytical procedures for the quantification of iron chelating drugs [8], [10], [13], [19], [20], [21], [22] and [23]. Those reporting the use of high performance liquid chromatography coupled with ultraviolet determination (HPLC UV) methods [8], [10], [13], [20] and [23], all applied methodology developed by Rouan in 2001 [19].…”

“…Those reporting the use of high performance liquid chromatography coupled with ultraviolet determination (HPLC UV) methods [8], [10], [13], [20] and [23], all applied methodology developed by Rouan in 2001 [19]. More recently liquid chromatographic methods based on mass spectrometry (LC MS MS) detection have been developed to this purpose [21] and [22], although MS facilities are not always available in standard hospital laboratories.…”

“…Chauzit et al [22] reported also the analysis of DFX metabolite, with the evidence that glucuronide not interferes with DFX at its retention time and that it do not convert usually into the parent drug, increasing the DFX concentration in vitro. Method developed by Rouan [19] permitted separation and simultaneous plasma determination of DFX and its iron complex in a range of concentrations from 0.25 to 20 μg/ml. In order to preserve the ratio between complex and total form, method required plasma samples storage at 4 °C immediately after collection, and all samples processing maintained at low temperature, procedures often difficult both for hospital setting and laboratory analytical routine.…”

Antiproliferative effect on HepaRG cell cultures of new calix[4]arenes. Part II

“…The various derivatives were compared to the tridentate hydroxyphenyltriazole ICL670 (Deferasirox, Exjade™) (I, Figure 1) [44]. Stock solutions of each molecule (10 mM) were prepared in DMSO.…”