Determination of a new active steroid by high performance liquid chromatography with laser-induced fluorescence detection following the pre-column derivatization

Xue-dong, Peng; Xu, Donghui; Jin, Jing; X, Mei; Lv, Jing; Xu, Shibo

doi:10.1016/j.ijpharm.2007.03.044

Cited by 4 publications

(1 citation statement)

References 25 publications

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“…HPLC-FLD was therefore ideal to study its PK–PD properties in rat plasma. A mixture of ZnSO 4 solution and acetonitrile were used for sample preparation to precipitate sample plasma proteins [ 70 ] and dansyl hydrazine was used as a derivatizing agent using trifluoroacetic acid (TFA) as a catalyst to enhance the yield. A C 18 reverse-phase column (150 mm × 4.6 mm, 5 mm) was used for the analysis and the limit of detection was 25 ng/mL (S/N = 10) using 200 μL of plasma.…”

Section: Pharmaceutical Applicationsmentioning

confidence: 99%

High Performance Liquid Chromatography (HPLC) with Fluorescence Detection for Quantification of Steroids in Clinical, Pharmaceutical, and Environmental Samples: A Review

et al. 2022

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Steroids are compounds widely available in nature and synthesized for therapeutic and medical purposes. Although several analytical techniques are available for the quantification of steroids, their analysis is challenging due to their low levels and complex matrices of the samples. The efficiency and quick separation of the HPLC combined with the sensitivity, selectivity, simplicity, and cost-efficiency of fluorescence, make HPLC coupled to fluorescence detection (HPLC-FLD) an ideal tool for routine measurement and detection of steroids. In this review, we covered HPLC-FLD methods reported in the literature for the steroids quantification in clinical, pharmaceutical, and environmental applications, focusing on the various approaches of fluorescent derivatization. The aspects related to analytical methodology including sample preparation, derivatization reagents, and chromatographic conditions will be discussed.

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Section: Pharmaceutical Applicationsmentioning

confidence: 99%