Determination of a 15437 bp nucleotide sequence around the inhA gene of Mycobacterium avium and similarity analysis of the products of putative ORFs

Labó, Monica; Gusberti, Laura; Rossi, Edda De; Speziale, Pietro; Riccardi, Giovanna

doi:10.1099/00221287-144-3-807

Cited by 14 publications

(8 citation statements)

References 51 publications

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“…Table 2 shows that the quantity of H37Rv M. tuberculosis transcripts decreased as a function of M⌽ activation, in agreement with a recent study (21). The M. tuberculosis-complex-specific genes whose expression was analyzed were MT10Sa, a structural gene (36), 35 kDa (27), ahpC (31), Ag85B and Ag85C (14), ESAT6 (35), rpoB (24) invB (19), sigF (15, 7), umaA2 (11), and eis (37). Of these 11 mycobacterial genes in resting M⌽ cDNA, 9 were expressed by strain H37Rv M. tuberculosis.…”

Section: Resultssupporting

confidence: 88%

Human Macrophage Gamma Interferon Decreases Gene Expression but Not Replication ofMycobacterium tuberculosis: Analysis of the Host-Pathogen Reciprocal Influence on Transcription in a Comparison of Strains H37Rv and CMT97

et al. 2001

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Mycobacterium tuberculosis is an intracellular pathogen that readily survives and replicates in human macrophages (M⌽). Host cells have developed different mycobactericidal mechanisms, including the production of inflammatory cytokines. The aim of this study was to compare the M⌽ response, in terms of cytokine gene expression, to infection with the M. tuberculosis laboratory strain H37Rv and the clinical M. tuberculosis isolate CMT97. Both strains induce the production of interleukin-12 (IL-12) and IL-16 at comparable levels. However, the clinical isolate induces a significantly higher and more prolonged M⌽ activation, as shown by reverse transcription-PCR analysis of IL-1␤, IL-6, IL-10, transforming growth factor beta, tumor necrosis factor alpha, and gamma interferon (IFN-␥) transcripts. Interestingly, when IFN-␥ transcription is high, the number of M. tuberculosis genes expressed decreases and vice versa, whereas no mycobactericidal effect was observed in terms of bacterial growth. Expression of 11 genes was also studied in the two M. tuberculosis strains by infecting resting or activated M⌽ and compared to bacterial intracellular survival. In both cases, a peculiar inverse correlation between expression of these genes and multiplication was observed. The number and type of genes expressed by the two strains differed significantly.

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Section: Resultssupporting

confidence: 88%

Human Macrophage Gamma Interferon Decreases Gene Expression but Not Replication ofMycobacterium tuberculosis: Analysis of the Host-Pathogen Reciprocal Influence on Transcription in a Comparison of Strains H37Rv and CMT97

et al. 2001

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“…Extremely low paralogies in buch and mlep are due to massive decay of genes in these genomes. secreted protein of 49.8 kDa, of which the C-terminal part is highly homologous (72%) to mycobacterial invasins (20). The molecular weight of the mature Nfa34810 is in good agreement with the 43-kDa protein, which plays a dominant role in the adherence and invasion of pulmonary epithelial cells by N. asteroides GUH-2 (21).…”

Section: Resultsmentioning

confidence: 88%

The complete genomic sequence of Nocardia farcinica IFM 10152

Ishikawa

Yamashita

Mikami

et al. 2004

Proc. Natl. Acad. Sci. U.S.A.

270

202

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We determined the genomic sequence of Nocardia farcinica IFM 10152, a clinical isolate, and revealed the molecular basis of its versatility. The genome consists of a single circular chromosome of 6,021,225 bp with an average G؉C content of 70.8% and two plasmids of 184,027 (pNF1) and 87,093 (pNF2) bp with average G؉C contents of 67.2% and 68.4%, respectively. The chromosome encoded 5,674 putative protein-coding sequences, including many candidate genes for virulence and multidrug resistance as well as secondary metabolism. Analyses of paralogous protein families suggest that gene duplications have resulted in a bacterium that can survive not only in soil environments but also in animal tissues, resulting in disease.N ocardia are filamentous-growing Gram-positive soil saprophytes that belong to the family Actinomycetales, which also includes clinically and industrially important genera such as Mycobacterium, Streptomyces, Corynebacterium, and Rhodococcus. Many species of Nocardia cause the disease nocardiosis in humans and animals on lung, central nervous system, brain, and cutaneous tissues (1), and a species Nocardia asteroides is suspected to be an etiological agent of Parkinson's disease (2). Nocardiosis is on the rise, with an estimated 109-136 new cases occurring annually in Japan (3) and 500-1,000 in the U.S. (www.cdc.gov͞ncidod͞dbmd͞diseaseinfo͞nocardiosis t.htm). However, there are only a few studies on the mechanisms of nocardial virulence. Nocardia species are resistant to many front-line antibiotics. Because treatment for nocardiosis relies heavily on chemotherapy, their intrinsic multiple drug resistance is a serious problem.Another feature of the nocardia is that many strains, even clinical isolates, also have the capability to produce bioactive molecules such as antibiotics (4, 5) and enzymes that are industrially important (6). A monobactam antibiotic, nocardicin, was isolated from Nocardia sp. (7), and a terpenoid brasilicardin with immunosuppressive activity was isolated from a clinical isolate (4).Nocardia farcinica IFM 10152 was isolated from the bronchus of a 68-year-old male Japanese patient. Despite the complicated taxonomy of the nocardia, the species N. farcinica was found to be nearly homogeneous (8). Subsequently, to elucidate the molecular basis of the versatility of the nocardia, we analyzed the genomic sequence of N. farcinica IFM 10152. MethodsGenome Sequencing and Assembly. The nucleotide sequence of the N. farcinica IFM 10152 genome was determined by a wholegenome shotgun strategy. We constructed small-insert (2 kb) and large-insert (10 kb) genomic libraries and generated 127,077 sequences (giving 9-fold coverage) from both ends of the genomic clones. Sequence assembly was carried out by using the PHRED͞PHRAP͞CONSED package (9). Remaining gaps were closed by transcriptional sequencing (Nippon Gene, Toyama, Japan) or by primer walking. There were no ambiguous nucleotides in the genomic sequence that we could determine. Consistency of the final assembly was confirmed in terms of res...

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“…paratuberculosis in infected sheep (14), PepA is likely to be expressed by M. tuberculosis growing in macrophages and to play an important role in facilitating survival. Rv1566c is homologous to the p60 invasion-associated protein of Listeria monocytogenes (37). In L. monocytogenes p60 is a murein hydrolase (65) which also participates in host cell invasion (31).…”

Section: Discussionmentioning

confidence: 99%

Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn 552′phoA In Vitro Transposition System

et al. 2000

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Determination of a 15437 bp nucleotide sequence around the inhA gene of Mycobacterium avium and similarity analysis of the products of putative ORFs

Cited by 14 publications

References 51 publications

Human Macrophage Gamma Interferon Decreases Gene Expression but Not Replication ofMycobacterium tuberculosis: Analysis of the Host-Pathogen Reciprocal Influence on Transcription in a Comparison of Strains H37Rv and CMT97

Human Macrophage Gamma Interferon Decreases Gene Expression but Not Replication ofMycobacterium tuberculosis: Analysis of the Host-Pathogen Reciprocal Influence on Transcription in a Comparison of Strains H37Rv and CMT97

The complete genomic sequence of Nocardia farcinica IFM 10152

Identification of Genes Encoding Exported Mycobacterium tuberculosis Proteins Using a Tn 552′phoA In Vitro Transposition System

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