Determination of 7-Hydroxymethotrexate in Human Plasma by Reversed Phase High-Performance Liquid Chromatography

Kinetics of 7-hydroxy-methotrexate (7-OH-MTX) excretion after high-dose methotrexate (MTX) (12 g/m2) therapy were monitored in 93 consecutive drug cycles of 19 adolescent patients with osteosarcoma. A reversed-phase HPLC method was used. Serum elimination was found to be monophasic with a mean half-life of 5.5 h. Shortly after the 4-h MTX infusion period 7-OH-MTX levels exceeded those of the parent compound. By 12 h after MTX infusion 7-OH-MTX levels were 16.5 times higher than those of MTX itself. Autostimulation of MTX metabolism leading to enhanced 7-OH-MTX production after repeated drug cycles was not observed. The production of 7-OH-MTX decreased significantly from the first to the last high-dose MTX cycle of the adjuvant chemotherapy protocol.

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Kinetics of 7-hydroxy-methotrexate after high-dose methotrexate therapy

Erttmann

Bielack

Landbeck

1985

Cancer Chemother. Pharmacol.

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Effect of oral cholestyramine on the elimination of high-dose methotrexate

Erttmann

Landbeck

1985

J Cancer Res Clin Oncol

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The anion exchange resin cholestyramine binds methotrexate (MTX) effectively in vitro. The binding capacity exceeds that of activated charcoal by a factor of 5.4. On two patients undergoing high-dose MTX therapy it is also shown that cholestyramine binds MTX in vivo. This leads to an enhanced non-renal excretion of MTX. Therefore, cholestyramine may be of clinical value in patients who develop renal function impairment whilst undergoing MTX therapy.

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Determination of methotrexate in serum by high-performance liquid chromatography

Aboleneen

Simpson

Backes

1996

Journal of Chromatography B: Biomedical Sciences and Applicatio

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Determination of 7-Hydroxymethotrexate in Human Plasma by Reversed Phase High-Performance Liquid Chromatography

Cited by 6 publications

References 6 publications

Kinetics of 7-hydroxy-methotrexate after high-dose methotrexate therapy

Kinetics of 7-hydroxy-methotrexate after high-dose methotrexate therapy

Effect of oral cholestyramine on the elimination of high-dose methotrexate

Determination of methotrexate in serum by high-performance liquid chromatography

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