Determination of 5‐hydroxytryptamine in serum by electrochemiluminescence detection with the aid of capillary electrophoresis

Abstract

A rapid, sensitive and simple electrochemiluminescence method for the determination of 5-hydroxytryptamine (5-HT) using capillary electrophoresis was proposed. The experimental parameters, including the detection potential, the concentration of Ru(bpy)(3)(2+), the concentration and pH of phosphate buffer for separation and detection, the injection voltage and time and the separation voltage on the determination of 5-HT, were optimized. Under the optimized conditions, the linear concentration range for 5-HT was 3.5 × 10(-9) -5.1 × 10(-3) mol/L, with a detection limit of 5 × 10(-10) mol/L. The relative standard deviations (RSDs) of the ECL intensity and the migration times for six continuous injections of 1.0 µmol/L 5-HT were 2.48% and 1.3%, respectively. The method was successfully applied to 5-HT assay in samples of human serum in 5 min and the extraction recoveries with spiked serum samples were over 94.4%.

MnCO3 as a New Electrochemiluminescence Emitter for Ultrasensitive Bioanalysis of β-Amyloid1–42 Oligomers Based on Site-Directed Immobilization of Antibody

“…41,42 The results were obtained by detecting different concentrations of Aβ in a human CSF sample. Subsequently, the ECL signal was detected by adding three standard concentrations of Aβ (1.00, 3.00, and 5.00 ng/mL) into original samples, and detection data are present in Table S2, with the RSDs of Aβ in human CSF samples equal to less than 6.45% and the recoveries 43 ranging from 98% to 102%, proving that the immunosensor could apply to the analysis of Aβ sensitively.…”

Electrochemiluminescent sensors as a screening strategy for psychoactive substances within biological matrices

“…[44][45][46][47][48] Previous ECL analysis within extracted and purified serum has demonstrated a similar ECL response from serum with the [Ru(bpy) 3 ] 2+ luminophore. 49 Further analysis of four of the common amino acids; proline, tryptophan, glutamine and lysine with the developed sensor with a supporting electrolyte of LiClO 4 , revealed their ability to behave as suitable ECL co-reactants, refer to Fig. S2.…”

Simultaneous quantification of 5-hydroxyindoleacetic acid and 5-hydroxytryptamine by capillary electrophoresis with quantum dot and horseradish peroxidase enhanced chemiluminescence detection

“…CE with laser-induced native fluorescence and UV detection has been applied for simultaneous determination of 5-HT and 5-HIAA [17][18][19][20]. CL detection has been proven to be one of the most sensitive detection technologies [21], there are already a few papers reporting the CE-CL analysis of 5-HT [22,23]. But, CE-CL analysis technology, at the best of my knowledge, has not been applied for simultaneous determination of 5-HT and 5-HIAA yet.…”