Determination of 5-chloro-7-iodo-8-quinolinol (clioquinol) in plasma and tissues of hamsters by high-performance liquid chromatography and electrochemical detection

Bondiolotti, Gianpietro; Pollera, C.; Pirola, Roberto; Bareggi, Silvio R.

doi:10.1016/j.jchromb.2006.04.014

Cited by 21 publications

(22 citation statements)

References 22 publications

(31 reference statements)

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“…Similar morphological changes to ours were identified in many antiscuticociliate agents regardless of their biocidal mechanisms (Iglesias et al., ; Paramá et al., ). For antiscuticociliate purposes, rapid absorption and ability to cross the blood–brain barrier seem to increase the clinical potential of an agent, because it is known that the target pathogen invades the brain very efficiently (Barregi & Cornelli, ; Bondiolotti et al., ). In infected olive flounder, scuticociliates are found in almost all organs, including the skin, fins, muscles, peritoneal cavity, kidney, pancreas and brain (Song et al., ).…”

Section: Discussionmentioning

confidence: 99%

“…The fish were anaesthetized with MS‐222 and the blood, liver, muscle (from the opposite site to injection) and brain were isolated. Plasma (100 μl) was prepared after centrifugation of blood at 3,500 × g for 20 min and isolated tissues of 100 mg each were processed for high‐performance liquid chromatography–electrochemical detection (Bondiolotti, Pollera, Pirola, & Bareggi, ). In brief, plasma was deproteinated by adding an equal volume of 0.6 N perchloric acid in the presence of 0.5 mg/ml Na 2 SO 3 and 0.25 mg/ml disodium EDTA.…”

Section: Methodsmentioning

confidence: 99%

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Effects of clioquinol on the scuticociliatosis‐causing protozoan Miamiensis avidus in olive flounder Paralichthys olivaceus

Park

Choi

et al. 2017

Journal of Fish Diseases

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Scuticociliatosis is a devastating and intractable protozoal disease in olive flounder, leading to a significant loss throughout the year. This study aimed to investigate a systemically effective antiscuticociliatosis agent for olive flounder for better absorption into the infected internal organs. The in vitro and in vivo antiscuticociliatosis effects of clioquinol (CQ) were examined after screening 30 biocidal agents against the highly pathogenic scuticociliate Miamiensis avidus. CQ was the most potent in vitro drug of those tested against cultured M. avidus. CQ was the least toxic in healthy olive flounder among the drugs that exhibit high potencies. In olive flounder, a single intramuscular injection of 40 mg/kg CQ significantly reduced mortality caused by artificial infection with M. avidus, and 10-20 mg/kg CQ increased fish survival times. CQ was also effective in naturally infected scuticociliatosis. Ciliate cell numbers were lower when CQ was injected in most organs, including the brain. CQ was well absorbed by the internal organs after intramuscular injection. This study suggests that CQ can be considered as a potential antiscuticociliatosis agent for systemic administration in olive flounder.

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Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Effects of clioquinol on the scuticociliatosis‐causing protozoan Miamiensis avidus in olive flounder Paralichthys olivaceus

Park

Choi

et al. 2017

Journal of Fish Diseases

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“…The clioquinol analysis was made using a new HPLC method with electrochemical detection described in detail elsewhere (Bondiolotti et al 2006). In brief, the chromatographic system consisted of a Shimadzu LC-6A pump (Kyoto, Japan), a refrigerated Dhimadzu SIL-9A autosampler and a Coulochem 5100A ESA dual potentiostat electrochemical detector (Bedford, MA).…”

Section: Methodsmentioning

confidence: 99%

“…The plasma and tissue samples were prepared as described by Bondiolotti et al (2006); the CSF samples (50 mL) were injected directly into the chromatograph.…”

Section: Clioquinol Analysismentioning

confidence: 99%

“…We here describe the pharmacokinetics of clioquinol, a chelator of many metals, such as copper and zinc, that has amyloidolytic properties and improves the symptoms of Alzheimer's disease (Bush 2003); in hamsters experimentally infected with scrapie, it reduces amyloid formation in-vitro (Formentin etal 2005) and delays the appearance of clinical symptoms and increases survival time in-vivo . Our analytical method is highly sensitive and specific (Bondiolotti et al 2006), and can therefore be used in pharmacokinetic studies in animals and man. As it does not require time-consuming sample preparation, it can also be used to monitor plasma clioquinol levels in patients.…”

Section: Repeated Administrationmentioning

confidence: 99%

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Pharmacokinetics and distribution of clioquinol in golden hamsters

Bondiolotti

Sala

Pollera

et al. 2007

Journal of Pharmacy and Pharmacology

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Clioquinol (5-chloro-7-iodo-8-quinolinol) is a zinc and copper chelator that can dissolve amyloid deposits and may be beneficial in Alzheimer's disease. Prion diseases are also degenerative CNS disorders characterised by amyloid deposits. The pharmacokinetics and tissue distribution of drugs active against prions may clarify their targets of action. We describe the pharmacokinetics of clioquinol in hamster plasma, spleen and brain after single and repeated oral or intraperitoneal administration (50 mg kg(-1)), as well as after administration with the diet. A single intraperitoneal administration led to peak plasma clioquinol concentrations after 15 min (Tmax), followed by a decay with an apparent half-life of 2.20 +/- 1.1 h. After oral administration, Tmax was reached after 30 min and was followed by a similar process of decay; the AUC(0-last) was 16% that recorded after intraperitoneal administration. The Cmax and AUC values in spleen after a single administration were about 65% (i.p.) and 25% (p.o.) those observed in blood; those in liver were 35% (p.o.) those observed in blood and those in brain were 20% (i.p.) and 10% (p.o.) those observed in plasma. After repeated oral doses, the plasma, brain and spleen concentrations were similar to those observed at the same times after a single dose. One hour after intraperitoneal dosing, clioquinol was also found in the ventricular CSF. Clioquinol was also given with the diet; its morning and afternoon concentrations were similar, and matched those after oral administration. No toxicity was found after chronic administration. Our results indicate that clioquinol, after oral administration with the diet, reaches concentrations in brain and peripheral tissues (particularly spleen) that can be considered effective in preventing prion accumulation, but are at least ten times lower than those likely to cause toxicity.

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Electrochemical and Hyphenated Electrochemical Detectors in Liquid Chromatography and Flow Injection Systems for Drug Compound Analysis

Özkan

Kauffmann

Zuman

2015

Monographs in Electrochemistry

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Determination of 5-chloro-7-iodo-8-quinolinol (clioquinol) in plasma and tissues of hamsters by high-performance liquid chromatography and electrochemical detection

Cited by 21 publications

References 22 publications

Effects of clioquinol on the scuticociliatosis‐causing protozoan Miamiensis avidus in olive flounder Paralichthys olivaceus

Effects of clioquinol on the scuticociliatosis‐causing protozoan Miamiensis avidus in olive flounder Paralichthys olivaceus

Pharmacokinetics and distribution of clioquinol in golden hamsters

Electrochemical and Hyphenated Electrochemical Detectors in Liquid Chromatography and Flow Injection Systems for Drug Compound Analysis

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