Determination of 3-monoiodotyrosine and 3,5-diiodotyrosine in newborn urine and dried urine spots by isotope dilution tandem mass spectrometry

Carcelén, Jesús Nicolás; Marchante-Gayón, Juan Manuel; Rodríguez-González, Pablo; Ballesteros, Alfredo; González, José Manuel; Juan, J.; Alonso, J. Ignacio García

doi:10.1039/d1an02203b

Cited by 4 publications

(4 citation statements)

References 41 publications

(119 reference statements)

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“…In neonatal screening for thyroid disease, urinary levels of MIT have been detected using isotope dilution tandem mass spectrometry, with values of 0.5−26 μg/g creatinine. 179 This finding demonstrates the potential of using MIT levels in neonatal screening as a tool to assess thyroid health. Overall, the ELC pathway, while minor in comparison to other metabolic processes, plays a crucial role in iodine recycling and can provide valuable insights into certain thyroid disorders, such as congenital hypothyroidism.…”

Section: Ether-linkage Cleavage (Elc)mentioning

confidence: 67%

“…Therefore, the level of MIT and DIT in urine could be helpful in clarifying whether disruption in iodine-recycle are responsible for hypothyroidism. In neonatal screening for thyroid disease, urinary levels of MIT have been detected using isotope dilution tandem mass spectrometry, with values of 0.5–26 μg/g creatinine . This finding demonstrates the potential of using MIT levels in neonatal screening as a tool to assess thyroid health.…”

Section: Thyroid Hormone Metabolism and Their Distributionmentioning

confidence: 99%

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Thyroid Hormone Biomonitoring: A Review on Their Metabolism and Machine-Learning Based Analysis on Effects of Endocrine Disrupting Chemicals

Chen,

Yu,

Yao

et al. 2024

Environ. Health

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The thyroid is an essential endocrine organ in human body, and thyroid hormones (THs) are pivotal signaling molecules and mediators in various physiological processes. THs, particularly in their free form, play a critical role in regulating body temperature and in the metabolism of lipid and glucose, making the maintenance of TH levels crucial for human health. THs undergo a series of metabolic processes, producing TH metabolites (THMs). THMs are significant in endocrine regulation, such as 3,5-diiothyronine (3,5-T2) and 3-iodothyronamine (3-T1AM), which exhibit activities akin to THs. The production and distribution of THMs are intricately linked to the function of specific organs and tissues, highlighting the need for advanced research into the determination and mechanisms of THMs in body. Exposure to endocrine disrupting chemicals (EDCs) can significantly affect the levels of thyroid stimulating hormone (TSH) and THs. This review utilizes machine learning to analyze epidemiological data, identifying potential EDCs that pose risks of hyperthyroidism and hypothyroidism. Additionally, it delves into the toxicological mechanisms of these EDCs, examining their effects on TH production, binding processes, related proteins, and metabolic enzymes. This approach effectively bridges the gap between epidemiological studies and toxicological researches, laying the groundwork for future research trends. By integrating epidemiological studies with machine learning, this review offers insightful perspectives on the potential risks associated with chemical exposure and underscores the necessity for further research in understanding the impact of EDCs on TH metabolism and TH-related health effects.

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Section: Ether-linkage Cleavage (Elc)mentioning

confidence: 67%

Section: Thyroid Hormone Metabolism and Their Distributionmentioning

confidence: 99%

Thyroid Hormone Biomonitoring: A Review on Their Metabolism and Machine-Learning Based Analysis on Effects of Endocrine Disrupting Chemicals

Chen,

Yu,

Yao

et al. 2024

Environ. Health

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“…Therefore, we performed the synthesis of natural and labelled angiotensin I (using 13 C 1 -L-valine) and the SPPS procedure [ 14 ] described. The synthesis products were characterized using the same LC conditions employed for the separation of amino acids.…”

Section: Resultsmentioning

confidence: 99%

“…The chromatographic separation of arginine, proline, valine, tyrosine, isoleucine, leucine and phenylalanine was carried out as butyl ester derivatives following a procedure described elsewhere [ 14 ].The experimental chromatographic conditions are shown in Table S1 of the Supporting Information. The same chromatographic conditions were employed for the separation of angiotensin I from its impurities.…”

Section: Methodsmentioning

confidence: 99%

Evaluation of different isotope dilution mass spectrometry strategies for the characterization of naturally abundant and isotopically labelled peptide standards

Nicolás Carcelén,

Potes Rodríguez,

González-Gago

et al. 2024

Anal Bioanal Chem

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Natural abundance and isotopically labelled tryptic peptides are routinely employed as standards in quantitative proteomics. The certification of the peptide content is usually carried out by amino acid analysis using isotope dilution mass spectrometry (IDMS) after the acid hydrolysis of the peptide. For the validation and traceability of the amino acid analysis procedure, expensive certified peptides must be employed. In this work we evaluate different IDMS alternatives which will reduce the amount of certified peptide required for validation of the amino acid analysis procedure. In this context, the characterization of both natural and isotopically labelled synthetic angiotensin I peptides was carried out. First, we applied a fast procedure for peptide hydrolysis based on microwave-assisted digestion and employed two certified peptide reference materials SRM 998 angiotensin I and CRM 6901-b C-peptide for validation of the hydrolysis procedure. The amino acids proline, leucine, isoleucine, valine, tyrosine, arginine and phenylalanine were evaluated for their suitability for peptide certification by IDMS by both liquid chromatography with tandem mass spectrometry (LC–MS/MS) and gas chromatography with mass spectrometry (GC)–MS/MS. Then, natural angiotensin I and 13C1-labelled angiotensin I were synthesized in-house and purified by preparative liquid chromatography. The concentration of the 13C1-labelled angiotensin I peptide was established by reverse IDMS in its native form using SRM 998 angiotensin I as reference. The concentration of the natural synthesized peptide was determined by IDMS both using the 13C1-labelled peptide in its native form and by amino acid analysis showing comparable results. Finally, the synthetic naturally abundant angiotensin I peptide was employed as “in-house” standard for the validation of subsequent peptide characterization procedures. Therefore, the novelty of this work relies on, first, the development of a faster hydrolysis procedure assisted by focused microwaves, providing complete hydrolysis in 150 min, and secondly, a validation strategy combining GC–MS and LC–MS/MS that allowed us to certify the purity of an in-house-synthesized peptide standard that can be employed as quality control in further experiments. Graphical abstract

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Simultaneous speciation analysis of arsenic and iodine in human urine by high performance liquid chromatography-inductively coupled plasma mass spectrometry

Zhou,

Wang,

Tang

et al. 2023

ANAL. SCI.

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Determination of 3-monoiodotyrosine and 3,5-diiodotyrosine in newborn urine and dried urine spots by isotope dilution tandem mass spectrometry

Abstract: Development of an analytical method for the determination of MIT and DIT in newborn urine and dried urine spots by Liquid Chromatography Isotope Dilution Tandem Mass Spectrometry capable of correcting analyte interconversion during sample preparation.

Cited by 4 publications

References 41 publications

Thyroid Hormone Biomonitoring: A Review on Their Metabolism and Machine-Learning Based Analysis on Effects of Endocrine Disrupting Chemicals

Thyroid Hormone Biomonitoring: A Review on Their Metabolism and Machine-Learning Based Analysis on Effects of Endocrine Disrupting Chemicals

Evaluation of different isotope dilution mass spectrometry strategies for the characterization of naturally abundant and isotopically labelled peptide standards

Simultaneous speciation analysis of arsenic and iodine in human urine by high performance liquid chromatography-inductively coupled plasma mass spectrometry

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