Determination of 2-hydroxyflutamide in human plasma by liquid chromatography–tandem mass spectrometry (LC–MS/MS): Application to a bioequivalence study on Chinese volunteers

Review of HPLC and LC–MS/MS assays for the determination of various nonsteroidal anti‐androgens used in the treatment of prostate cancer

“…Zheng et al () reported a method quantify 2‐hydroxyflutamide in human plasma. Rather than using acetonitrile (most commonly used) or trifluoroacetic acid as protein precipitants, methanol was considered as the choice for simple protein precipitation because it provided good recovery and minimized the occurrence of matrix effects.…”

“…Three commercially available drugs, namely acyclovir, tegafur and isinopril, were considered for internal standard evaluation. The choice of tegafur over the two drugs as the internal standard was justified because both chromatographic and mass spectrometric attributes were similar to 2‐hydroxyflutamide and additionally it provided high level of sensitivity for the analysis (Zheng et al, ).…”