Determination of 17‐Monochloroacetylajmaline and Its Metabolite in Plasma by TLC Fluorescence Detection

Dombrowski, Lawrence J.; Crain, A.V.R.; Browning, Robert S.; Pratt, Edward L.

doi:10.1002/jps.2600640414

Cited by 9 publications

(2 citation statements)

References 3 publications

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“…fluorescence of twenty drug-free plasma extracts from different subjects was equivalent to 0.038 + 0.013,g ml-' ajmaline. Thus, the sensitivity of this method is well within the sensitivity (0.1 g,g ml-') of the chromatographic method (Dombrowski et al, 1975).…”

Section: Recovery Sensitivity and Reproducibilitysupporting

confidence: 64%

“…Several methods have been developed for the measurement of ajmaline in plasma based on polarography, thin-layer chromatography or colorimetry (Tsarenko, Orlov & Schraiber, 1963; Yalcindag, 1969;Sobiczewska & Borkowski, 1970;Kabela, Jalife & Pastclin, 1974). A more sensitive and specific method for the measurement of ajmaline and its derivative 17-monochloroacetylajmaline (MCAA) was developed by Dombrowski, Crain, Browning & Pratt (1975) using thin-layer chromatography and fluorescence detection. However, in the absence of MCAA we have found that ajmaline concentrations can be determined by direct measurement of the fluorescence of plasma extracts adjusted to pH 6.5.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A spectrofluorometric method for the determination of ajmaline in plasma.

Welman¹,

Curry²,

Krikler³

et al. 1977

Brit J Clinical Pharma

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1 Ajmaline was found to have maximum fluorescence at neutral pH with 300 nm excitation and 365 nm emission wavelengths (corrected). 2 The fluorescence intensity had a linear relationship to concentration up to 50 gg ml-' and the recovery of ajmaline after extraction from plasma was 92.5 + 3%. 3 Extraction of drug-free plasma and of samples containing known concentrations of ajmaline showed that drug levels in the range found clinically could be measured accurately by fluorimetry. 4 Serial plasma ajmaline concentrations were measured in a subject after intravenous injection of ajmaline (50 mg). The rates of plasma clearance of the drug were found to be similar to those obtained in previous studies.

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Section: Recovery Sensitivity and Reproducibilitysupporting

confidence: 64%

Section: Introductionmentioning

confidence: 99%

A spectrofluorometric method for the determination of ajmaline in plasma.

Welman¹,

Curry²,

Krikler³

et al. 1977

Brit J Clinical Pharma

View full text Add to dashboard Cite

show abstract

The pharmacokinetics and organ distribution of ajmaline and quinidine in the mouse

Iven

1977

Naunyn-Schmiedeberg's Arch. Pharmacol.

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After i.v. infusion into mice (lasting 10 s) the time courses of ajmaline and quinidine concentrations in blood, heart, lung, liver, and brain were studied. The drugs were assayed by a spectrofluorophotometric procedure. Blood concentration data obtained were fitted graphically and calculations were performed in accordance with an open two compartment model. Blood kinetic data were very similar for both alkaloids. A rapid distribution phase with a t0.5alpha of 3.0 min for ajmaline and 2.5 min for quindine was followed by a disposition phase with a t0.5beta of 16 min for ajmaline and 20 min for quinidine. High tissue accumulation of both alkaloids was found in lung, liver, and heart and this is also reflected by the volume of distribution Vdbeta, which was 136 ml for ajmaline and 116 ml for quinidine (body weight of the mice = 31 g). With equilibrium dialysis a 62% binding of ajmaline and a 77% binding of quinidine to mouse blood constituents was found. Both drugs were highly metabolized since only 5% of a given dose was excreted unchanged in the urine.

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Use of derivative spectrofluorometry in the analysis of ajmaline in drug dosage forms

Khveshchuk¹,

Рудакова²

1995

Pharm Chem J

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Determination of 17‐Monochloroacetylajmaline and Its Metabolite in Plasma by TLC Fluorescence Detection

Cited by 9 publications

References 3 publications

A spectrofluorometric method for the determination of ajmaline in plasma.

A spectrofluorometric method for the determination of ajmaline in plasma.

The pharmacokinetics and organ distribution of ajmaline and quinidine in the mouse

Use of derivative spectrofluorometry in the analysis of ajmaline in drug dosage forms

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