Determination of 14 Chemical Constituents in the Traditional Chinese Medicinal Preparation Huangqin-Tang by High Performance Liquid Chromatography.

Zuo, Feng; Zhou, Zhongming; Liu, Mei‐Lan

doi:10.1248/bpb.24.693

Cited by 33 publications

(28 citation statements)

References 16 publications

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“…[8][9][10][11][12][13][14] They were converted into the metabolites baicalein (B), paeonimetabolins (PM) and glycyrrhetinic acid (GA) by HIF in vitro, which were absorbed into body. [15][16][17][18][19][20][21][22][23] In our previous paper, 24) we established the method to simultaneously analyze the 14 chemical constituents of Huangqin-Tang by HPLC. Among these compounds, there are several pairs of original compounds and metabolites.…”

mentioning

confidence: 99%

Metabolism of Constituents in Huangqin-Tang, a Prescription in Traditional Chinese Medicine, by Human Intestinal Flora

Zuo

Zhou

Yan

et al. 2002

Biological & Pharmaceutical Bulletin

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confidence: 99%

Metabolism of Constituents in Huangqin-Tang, a Prescription in Traditional Chinese Medicine, by Human Intestinal Flora

Zuo

Zhou

Yan

et al. 2002

Biological & Pharmaceutical Bulletin

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“…Scutellaria baicalensis contains many flavonoids (15), which are known to show estrogenic action following the binding to ER (16,17). The results from whole cell and cell-free binding assays showed that Scutellaria baicalensis had no direct action on estrogen binding sites in B-1F cells.…”

Section: Discussionmentioning

confidence: 97%

“…Scutellaria baicalensis contains flavonoids, mainly baicalin, baicalein and wogonin (15). These flavonoids are known to bind to estrogen receptor (ER) and cause estrogen-like action (16,17).…”

Section: Effect Of Scutellaria Baicalensis and Saiboku-to On The Bindmentioning

confidence: 99%

Proliferation of estrogen-responsive mouse tumor cell line B-1F stimulated by Saiboku-to, but inhibited by Scutellaria baicalensis, a component of Saiboku-to

Murashima

Yamasaki²,

Nishizawa³

et al. 2009

Oncol Rep

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Abstract. We have demonstrated that the proliferation of estrogen-responsive mouse Leydig tumor cell line B-1F is induced via suppression of 5-lipoxygenase activity followed by decrease of leukotrienes (LTs). Additionally, it has been reported that LTD4 induces apoptosis in B-1F cells. In this study, we examined effects of Saiboku-to, a traditional Chinese medicine having suppressive activities for LT production and release, on the proliferation. Saiboku-to promoted, but Scutellaria baicalensis, one of components (herbs) of Saiboku-to, significantly inhibited the proliferation of B-1F cells in vitro and in vivo. The action of Scutellaria baicalensis in B-1F cells was studied in more detail. Although Scutellaria baicalensis consists of flavonoids, iridoids, volatile oils and others, it and its major constituents had no direct effect on estrogen binding sites in B-1F cells. B-1F cells treated with Scutellaria baicalensis showed morphological changes such as nuclear aggregation and fragmentation. DNA fragmentation was also observed, indicating that Scutellaria baicalensis induces apoptosis in B-1F cells and that it or its constituents might be a good resource for searching new drugs, especially anti-cancer drugs. Moreover, Saiboku-to promoted B-1F cell proliferation, but Scutellaria baicalensis inhibited it, showing complexity of action of traditional Chinese medicines.

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“…In our experiments Scutellaria baicalensis slightly inhibited expression of nF-κB p65 in B-1F cells in the presence of e2. Moreover, Scutellaria baicalensis contains many flavonoids and its major constituents are baicalin, baicalein, wogonin, neowogonin and skullcapflavone (6). Baicalein and wogonin inhibited B-1F cell proliferation and induced apoptosis.…”

Section: Discussionmentioning

confidence: 99%

“…In the studies on the effects of saiboku-to on cell proliferation, we have found that Scutellaria baicalensis, one component of saiboku-to, suppresses the proliferation of estrogen-responsive mouse tumor cell line B-1F. Scutellaria baicalensis also contains many flavonoids (6). Recent studies suggest that Scutellaria baicalensis effectively arrests cell cycle and induces apoptosis of tumor cell lines (3,7,8).…”

Section: Introductionmentioning

confidence: 99%

Possible mechanism of growth inhibition by Scutellaria baicalensis in an estrogen-responsive mouse tumor cell line

Murashima

Katayama²,

Shojiro³

et al. 2011

Oncol Rep

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Abstract.We have studied the effects of saiboku-to, a traditional chinese medicine having suppressive activities for leukotriene production and release, on the proliferation of the estrogen-responsive mouse leydig tumor cell line B-1F. In our previous reports, it is shown that saiboku-to promotes, but Scutellaria baicalensis, one of the components (herbs) of Saiboku-to, significantly inhibits the proliferation of B-1F cells in vitro and in vivo, and induces DnA fragmentation and morphological changes such as nuclear aggregation and fragmentation. In this study, we examined telomerase activity, cell cycle, polyunsaturated fatty acid metabolism and expression of nuclear factor κB (nF-κB) in order to determine the mechanism of growth inhibition in B-1F cells treated with Scutellaria baicalensis. telomerase activity was decreased in a dose-dependent manner in treated B-1F cells. cellular populations in the sub-g0/g1 and g2/M phases were increased, but those in M phase had no change. Although cyclin D1 mrnA was highly expressed in the presence of estradiol (e2), cyclin A and E mRNA levels did not significantly change. When B-1F cells were treated with Scutellaria baicalensis, expression of cyclin D1 was suppressed and that of p21 was inversely increased. Moreover, Scutellaria baicalensis influenced arachidonic and linoleic acid metabolism, and increased production of 13(s)-HoDe. In the presence of e2 Scutellaria baicalensis decreased expression of nF-κB p65 to 0.71-fold in B-1F cells. these results show that Scutellaria baicalensis might induce cell cycle arrest at g1 phase and apoptosis via inhibition of telomerase activity, changes of enzymatic activities in polyunsaturated fatty acid metabolism and suppression of nF-κB.

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Determination of 14 Chemical Constituents in the Traditional Chinese Medicinal Preparation Huangqin-Tang by High Performance Liquid Chromatography.

Cited by 33 publications

References 16 publications

Metabolism of Constituents in Huangqin-Tang, a Prescription in Traditional Chinese Medicine, by Human Intestinal Flora

Metabolism of Constituents in Huangqin-Tang, a Prescription in Traditional Chinese Medicine, by Human Intestinal Flora

Proliferation of estrogen-responsive mouse tumor cell line B-1F stimulated by Saiboku-to, but inhibited by Scutellaria baicalensis, a component of Saiboku-to

Possible mechanism of growth inhibition by Scutellaria baicalensis in an estrogen-responsive mouse tumor cell line

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