Determination Extraction Conditions for Maximum Phenolic Compounds in the Kiwifruit Using Response Surface Design

Abstract

The study aims to determine the extraction parameters for maximal phenolic compounds and antioxidant activity from kiwifruit by using response surface methodology (RSM). Box-Behnken experimental design of RSM was applied to determine the effect of extraction parameters. Three independent variables were selected at three levels: methanol concentration (A: 60-75-90 %), ultrasound time (B: 5-10-15 min.), and mass of sample (C: 1-2-3 g). Conditions for maximum phenolic yield were found as A = 90%, B= 7.71 min, and C = 3 g, under this condition, DPPH of 88.96 %, ABTS of 739.07 mg/100g FW and TPC of 299.14 mg/100g fresh weight has been obtained.