Determination and properties of actively metabolizing heterotrophic bacteria in the sea, investigated by means of micro-autoradiography

Höppe, Henning A.

doi:10.1007/bf00389190

Cited by 158 publications

(85 citation statements)

References 19 publications

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“…The assimilation of phosphate is depicted in Figure 5-5 for the con- (Ferguson and Rublee, 1976;Hoppe, 1976;Watson et al, 1977). Therefore, at each sampling point for the organic compounds duplicate samples were filtered for fractionation and a third aliquot was filtered through a 0.2 l.m membrane filter, which may be assumed to retain all the cell s. A compari son of the sum of the rad i oactivity in each of the biochemical fractions for each sample with its associated whole cell filter is found in Appendix The second control is the reproducibility of subcellular fractionation of isotopically-labeled cells.…”

Section: Tota 1 Uptake Of Phosphatementioning

confidence: 99%

Assimilatory sulfur metabolism in marine microorganisms

Cuhel¹

1980

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Section: Tota 1 Uptake Of Phosphatementioning

confidence: 99%

Assimilatory sulfur metabolism in marine microorganisms

Cuhel¹

1980

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“…Yet, the majority of marine bacteria are not amenable to cultivation, excluding potentially important representatives of natural bacterial communities from laboratory experiments (Jannasch and Jones, 1959;Hoppe, 1976;Bianchi and Giuliano, 1996). In addition, it is important to establish the extent to which swimming and chemosensory capabilities of laboratory isolates reflect their natural state, and how isolates respond to chemoattractants in the presence of natural populations.…”

Section: Introductionmentioning

confidence: 99%

Chemotaxis by natural populations of coral reef bacteria

et al. 2015

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Corals experience intimate associations with distinct populations of marine microorganisms, but the microbial behaviours underpinning these relationships are poorly understood. There is evidence that chemotaxis is pivotal to the infection process of corals by pathogenic bacteria, but this evidence is limited to experiments using cultured isolates under laboratory conditions. We measured the chemotactic capabilities of natural populations of coral-associated bacteria towards chemicals released by corals and their symbionts, including amino acids, carbohydrates, ammonium and dimethylsulfoniopropionate (DMSP). Laboratory experiments, using a modified capillary assay, and in situ measurements, using a novel microfabricated in situ chemotaxis assay, were employed to quantify the chemotactic responses of natural microbial assemblages on the Great Barrier Reef. Both approaches showed that bacteria associated with the surface of the coral species Pocillopora damicornis and Acropora aspera exhibited significant levels of chemotaxis, particularly towards DMSP and amino acids, and that these levels of chemotaxis were significantly higher than that of bacteria inhabiting nearby, non-coral-associated waters. This pattern was supported by a significantly higher abundance of chemotaxis and motility genes in metagenomes within coral-associated water types. The phylogenetic composition of the coral-associated chemotactic microorganisms, determined using 16S rRNA amplicon pyrosequencing, differed from the community in the seawater surrounding the coral and comprised known coral associates, including potentially pathogenic Vibrio species. These findings indicate that motility and chemotaxis are prevalent phenotypes among coral-associated bacteria, and we propose that chemotaxis has an important role in the establishment and maintenance of specific coral-microbe associations, which may ultimately influence the health and stability of the coral holobiont.

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“…Bacteria are now know to comprise a dynamic pool of organic carbon in the sea, yet no grazing organisms have so far convincingly been shown to be capable of keeping bacterial densities within the range of 0.5 to 2 X 106 ml-' which is typical for marine plankton (Ferguson and Rublee, 1976;Hoppe, 1976;Hagstrom et al, 1979). In order to evaluate the role of the microflagellates, it is necessary not only to quantify them in nature, but also to obtain a qualitative and quantitative understanding of their biology, food uptake and bioenergetics.…”

Section: Introductionmentioning

confidence: 99%

Ecology of Heterotrophic Microflagellates. II. Bioenergetics and Growth

Fenchel¹

1982

Mar. Ecol. Prog. Ser.

477

322

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The bioenergetics of growth of 6 species of heterotrophic (bacterivorous) microflagellates was studied in batch cultures. Maximum growth rates varied between 0.15 and 0.25 h-' among the species, but growth as slow as 0.028 h-' could be maintained. Yield (gross growth efficiency) was found to be ca. 30 % (volume) or, in 2 cases, 34 and 43 % (C basis) respectively; net growth efficiency (C) was 60 %. Respiratory and growth rates were directly proportional to consumption rate within the range of growth rates studied. Clearance (volume of water cleared for bacteria per unit time and measured in fractions of cell volume) ranged from about 5 X 104 to about 106 h-'; this large variation can in part be explained as a consequence of specialization to different food particle slzes. In general, the parameter values suggest that the type of flagellates studied may grow on and possibly control the concentration of bacteria found in marine plankton.

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Determination and properties of actively metabolizing heterotrophic bacteria in the sea, investigated by means of micro-autoradiography

Cited by 158 publications

References 19 publications

Assimilatory sulfur metabolism in marine microorganisms

Assimilatory sulfur metabolism in marine microorganisms

Chemotaxis by natural populations of coral reef bacteria

Ecology of Heterotrophic Microflagellates. II. Bioenergetics and Growth

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