Determination and pharmacokinetic study of echinatin by UPLC-MS/MS in rat plasma

Li, Tianran; Ye, Weijian; Huang, Binge; Chen, Xixi; Lin, Yijing; Wen, Congcong; Wang, Xianqin

doi:10.1016/j.jpba.2019.02.023

Cited by 26 publications

(11 citation statements)

References 11 publications

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“…1A), a retrochalcone, is an active ingredient of licorice and the main active form with pharmacokinetic function. 15 Studies have shown that Ech has a wide range of biological properties, including anti-inflammatory and anti-tumor effects. 16 Importantly, Liang et al 17 confirmed that Ech may undergo electron transfer and proton transfer to cause antioxidant effects.…”

Section: Introductionmentioning

confidence: 99%

Echinatin mitigates H2O2-induced oxidative damage and apoptosis in lens epithelial cells via the Nrf2/HO-1 pathway

Ran¹,

Liu²,

Wu³

2021

Adv Clin Exp Med

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Background.Oxidative stress has been reported to be an early factor in the development of cataracts. Echinatin (Ech) is an active ingredient of licorice that exhibits antioxidant effects. Objectives.To investigate the effects of Ech on oxidative stress-induced lens epithelial cell (LEC) damage. Materials and methods.Human lens epithelial B3 cells (HLECs) were exposed to hydrogen peroxide (H 2 O 2 ) and were pretreated with or without Ech. For rescue experiments, ML385, an inhibitor of the Nrf2 pathway, was added into the medium.Results. Echinatin reversed the H 2 O 2 -induced reduction of cell viability in B3 cells. Additionally, H 2 O 2 induced oxidative stress, evidenced by an increase of reactive oxygen species (ROS) and malondialdehyde (MDA) levels, and a decrease in superoxide dismutase (SOD) and catalase (CAT) levels, which could be abolished by Ech. Echinatin treatment also reduced HLEC apoptosis induced by H 2 O 2 . In addition, Ech pretreatment promoted Bcl-2 expression, and suppressed Bax and caspase-3 expression levels, in H 2 O 2 -treated B3 cells. Moreover, H 2 O 2 significantly reduced Nrf2 nuclear localization, as well as HO-1 and NQO1 expression, which could be reversed by Ech. Inhibition of Nrf2 by ML385 aggravated H 2 O 2 -induced oxidative damage and apoptosis in HLECs, and the protective effects of Ech on H 2 O 2 -induced oxidative damage and apoptosis could be restored by ML385. Conclusions.Echinatin mitigates H 2 O 2 -induced oxidative damage and apoptosis in HLECs via the Nrf2/HO-1 pathway, suggesting that Ech may be a potential drug for the treatment of cataracts.

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Section: Introductionmentioning

confidence: 99%

Echinatin mitigates H2O2-induced oxidative damage and apoptosis in lens epithelial cells via the Nrf2/HO-1 pathway

Ran¹,

Liu²,

Wu³

2021

Adv Clin Exp Med

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“…e indole alkaloids of Uncaria rhynchophylla are the main active ingredients. e distribution of drugs in the body is closely related to the pharmacological effects of drugs, and their distribution determines the strength and duration of the effect [13][14][15]. It is still unclear whether hirsutine and hirsuteine can be absorbed into the blood and its metabolism and tissue distribution.…”

Section: Introductionmentioning

confidence: 99%

Tissue Distribution of Hirsutine and Hirsuteine in Mice by Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry

Zhou

Chen

2020

Journal of Analytical Methods in Chemistry

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Hirsutine and hirsuteine were two alkaloid monomers extracted from the traditional Chinese medicine Uncaria rhynchophylla, which have pharmacological effects such as antihypertension, anti-infection, and heart protection. An ultrahigh-performance liquid chromatography-mass spectrometry was established for the determination of hirsutine and hirsuteine in tissues (liver, kidney, heart, spleen, brain, and lung), and their absorption, distribution, and metabolism were studied for providing information on its pharmacological mechanism. UPLC BEH C18 column (2.1 mm × 100 mm, 1.7 μm) was used for chromatographic separation. The mobile phase was acetonitrile-0.1% formic acid, with a gradient elution, and the total run time was 4 min. Electrospray was used in the positive ion mode, and the multiple reaction monitoring (MRM) mode was for quantification. The acetonitrile precipitation method was used to remove protein-treated mouse plasma and tissue homogenate samples. In the concentration range of 2–5000 ng/g, hirsutine and hirsuteine in tissues showed good linearity (r > 0.995), and the lower limit of quantification was 2 ng/g. In the plasma and liver tissues, the interday and intraday precision of hirsutine and hirsuteine was less than 15%, the accuracy was between 90.9% and 110.1%, and the average recovery was better than 73.0%. The matrix effect was between 86.2% and 104.7%. The results showed that the precision, accuracy, recovery, and matrix effects meet the requirements for the study on the distribution of hirsutine and hirsuteine. After intraperitoneal administration of 10 mg/kg hirsutine and hirsuteine in mice, the distribution levels were highest in liver and kidney tissues, followed by the spleen and lung. Hirsutine and hirsuteine were low in brain tissue, but had obvious distribution, suggesting that they may pass through the blood-brain barrier.

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“…Briefly, 50 µ L of rat plasma and 150 µ L of acetonitrile (containing midazolam 50 ng/mL) were added to a 1.5 mL Eppendorf tube, mixed, vortexed for 15 s (XW-80A Vortex Mixer, Kylin-Bell Lab Instruments, Haimen, China), and then centrifuged. After centrifugation at 13000 r/min for 10 min (Centrifuge 5804 R, Eppendorf AG, Hamburg, Germany), 150 µ L of the supernatant was transferred for glass vial detection [ 22 , 23 ].…”

Section: Methodsmentioning

confidence: 99%

Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

Qiu

Liang

et al. 2022

International Journal of Analytical Chemistry

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This study established an ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method to study the pharmacokinetics of four antiepileptic drugs, lamotrigine, oxcarbazepine, lacosamide, and topiramate, in rats after oral administration. The gradient elution was performed on a UPLC HSS T3 (2.1 mm × 100 mm, 1.8 μm) column with acetonitrile-0.1% formic acid as the mobile phase at a flow rate of 0.4 mL/min. Protein precipitation by acetonitrile was adopted for plasma sample pretreatment. Electrospray- (ESI-) positive/negative ion switching and multiple reaction monitoring (MRM) modes were adopted for ion quantitative determination of antiepileptic drugs. UPLC-MS/MS detection and Drug and Statistics (DAS) software fitting were performed to blood samples collected from rats after oral administration of lamotrigine, oxcarbazepine, lacosamide, and topiramate (5 mg/kg). All drugs examined showed linearity within 5–5000 ng/ml (R2 > 0.9987), the intraday accuracy was within 92%–108%, and the interday accuracy was within 93%–109%. The relative standard deviations (RSD) of intraday and interday were less than 15%. The matrix effect was within 91%–105%, and the recovery was better than 88%. The established UPLC-MS/MS method was successfully applied to the pharmacokinetic study of lamotrigine, oxcarbazepine, lacosamide, and topiramate in rats.

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Determination and pharmacokinetic study of echinatin by UPLC-MS/MS in rat plasma

Cited by 26 publications

References 11 publications

Echinatin mitigates H2O2-induced oxidative damage and apoptosis in lens epithelial cells via the Nrf2/HO-1 pathway

Echinatin mitigates H2O2-induced oxidative damage and apoptosis in lens epithelial cells via the Nrf2/HO-1 pathway

Tissue Distribution of Hirsutine and Hirsuteine in Mice by Ultrahigh-Performance Liquid Chromatography-Mass Spectrometry

Simultaneous Determination of Lamotrigine, Oxcarbazepine, Lacosamide, and Topiramate in Rat Plasma by Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry

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