Determination and Confirmation of the Antiviral Drug Amantadine and Its Analogues in Chicken Jerky Pet Treats

Turnipseed, Sherri B; Storey, Joseph M; Andersen, Wendy C.; Filigenzi, Michael S.; Heise, Andrea; Lohne, Jack J.; Madson, Mark R.; Cerić, Olgica; Reimschuessel, Renate

doi:10.1021/acs.jafc.5b02416

Cited by 22 publications

(4 citation statements)

References 12 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Analyses of these drugs in different matrices have been reported in the literature. These include determinations of memantine hydrochloride in bulk drug and in tablets by sequential injection analysis , analyses of amantadine in chicken muscle by GC and measurements of memantine, amantadine and rimantadine in animal tissues by HPLC–MS and UHPLC–MS . HPLC with optical detectors (e.g., UV‐visible or fluorescence detectors) usually require some derivatization with labeling reagents (e.g., o ‐phthaldialdehyde and 2,3‐naphtalenedicarboxaldehyde) to enable the analyte detection.…”

Section: Introductionmentioning

confidence: 99%

In‐capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Prapatpong

Nuchtavorn

Macka

et al. 2018

J of Separation Science

View full text Add to dashboard Cite

In-capillary derivatization using fluorescamine as the labeling reagent was proposed to enhance the detectability of adamantine drugs (memantine, amantadine and rimantadine) by spectrophotometric detection. Fluorescamine and the drugs were delivered to the capillary electrophoresis instrument at a ratio of 10:1 by zone injection. The derivatization reaction occurred following the application of voltage (20 kV). The derivatized products, hydrolyzed- fluorescamine and excess fluorescamine were separated in 7 min using 100 mM borate buffer (pH 10.0) containing 0.1% w/v of Brij®-35 and 20% v/v of acetonitrile. Validation data showed good linearity (r > 0.98), precision (%RSDs < 3.4), and accuracy (recoveries ranging from 98.0 to 102.0%). The detection and quantitation limits are in the range of 6.0-8.5 and 18-25 μM, respectively. The validation data is comparable to reported methods, however, the current method offers better precision with enhanced sensitivity (up to six times). Applications of the method show percent labeled amounts found in the studied samples within 100.6-109.3%, which complied with the United States Pharmacopeia limit (90.0-110.0%). The method was simple, rapid and, automated, which required no extra instrumentation or skillful operators.

show abstract

Section: Introductionmentioning

confidence: 99%

In‐capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Prapatpong

Nuchtavorn

Macka

et al. 2018

J of Separation Science

View full text Add to dashboard Cite

show abstract

“…After the solution was vortexed for 30 sec and centrifuged at 11 963 g for 10 min, the supernatant was collected for HPLC-MS/MS detection. The detection method was optimized based on the methods described in development of the HPLC-MS/MS method for detecting amantadine in chicken (Arndt et al, 2005;Yan et al, 2013;Wu et al, 2014;Zhang & Du, 2014;Turnipseed et al, 2015;Mu et al, 2016).…”

Section: Sample Preparationmentioning

confidence: 99%

Study on the abuse of amantadine in tissues of broiler chickens by HPLC‐MS/MS

You

Yang

Zhao

et al. 2017

Vet Pharm & Therapeutics

View full text Add to dashboard Cite

To evaluate the residual target tissues for better monitoring of amantadine abuse in broiler chickens, 22-day-old commercial Arbor Acres broiler chickens were, respectively, fed with 10, 20, and 40 mg/kg of amantadine for five consecutive days. Plasma, breast, and liver tissue samples from the chickens were collected 0, 4, 16, 24, 48, 96, 144, and 312 h after amantadine withdrawal. The high-performance liquid chromatography-tandem mass spectrometry method was used to detect the concentrations of amantadine. The highest concentration was found in the chicken liver and it took the longest time for amantadine to vanish by metabolism. In the high-dose group, amantadine residues were still detected 312 h after amantadine withdrawal. As the amantadine dose increased, amantadine residues in the chicken liver were more slowly to disappear than in other tissues. Even if approximately the same concentration of amantadine residues was found in chicken breast and plasma samples, it took a shorter time before the residues were eliminated. In the medium- and high-dose groups, the concentrations of amantadine residues in chicken liver samples were substantially higher than those in chicken breast and plasma samples, and it took more time to eliminate them. Therefore, the chicken liver can be used as a target tissue to detect illegal use of amantadine.

show abstract

“…Amantadine (AMD), a common antiviral agent, has been widely used to treat viral infections in the poultry industry. , However, illegal abuse of AMD causes the presence of its residue in animal-derived products. Humans consuming food with this AMD residue will produce drug resistance. , Therefore, AMD has been prohibited as an antiviral drug in poultry farming in many countries including China since 2005 .…”

Section: Introductionmentioning

confidence: 99%

Discovery and Optimization of an Aptamer and Its Sensing Ability to Amantadine Based on SERS via Binary Metal Nanoparticles

Duan

Ren

Lyu

et al. 2022

J. Agric. Food Chem.

View full text Add to dashboard Cite

With the growing concern of illegal abuse of amantadine (AMD) and its potential harmful impact on humans, detection of AMD has become an urgent food safety and environmental topic. Biosensing is a promising method for this, but the effective recognition of AMD still remains a challenge. Herein, we isolated an aptamer (Am-20) for AMD through a 14-round iterative selection based on capture-SELEX. The preliminary interaction mechanism between AMD and Am-20 was clarified with the help of docking simulations. Facilitated by a base mutation and truncation strategy, an optimized aptamer Am-20-1 with a short length of 62-mer was obtained, which exhibited competitive affinity with a K d value of 33.90 ± 5.16 nM. A structure-switching SERS-based aptasensor based on Am-20-1 was then established for AMD quantification via a binary metal nanoparticle-embedded Raman reporter substrate (AuNRs@ATP@AgNPs). The fabricated strategy showed a wide linear range (0.005∼25 ng/mL) and a low limit of detection (0.001 ng/mL) for AMD determination. We envision that the novel aptamer identified in this study will provide a complementary tool for specific recognition and detection of AMD and could assist in the supervision of illegal abuse of AMD.

show abstract

Determination and Confirmation of the Antiviral Drug Amantadine and Its Analogues in Chicken Jerky Pet Treats

Cited by 22 publications

References 12 publications

In‐capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

In‐capillary derivatization with fluorescamine for the rapid determination of adamantane drugs by capillary electrophoresis with UV detection

Study on the abuse of amantadine in tissues of broiler chickens by HPLC‐MS/MS

Discovery and Optimization of an Aptamer and Its Sensing Ability to Amantadine Based on SERS via Binary Metal Nanoparticles

Contact Info

Product

Resources

About