Determination and analysis of the genome sequence of Spodoptera littoralis multiple nucleopolyhedrovirus

Breitenbach, Jonathan E.; El-Sheikh, El-Sayed A.; Harrison, Robert L.; Rowley, Daniel L.; Sparks, Michael E.; Gundersen‐Rindal, Dawn E.; Popham, Holly J.R.

doi:10.1016/j.virusres.2012.11.016

Cited by 22 publications

(28 citation statements)

References 85 publications

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“…The nucleotide identity of the polyhedrin, DNApol, pif-2 and lef-8 sequences of SpltNPVPak-BNG and SpltNPV-G1 were 94%, 87%, 89% and 88%, respectively (Table 6). The sequences of the 'ORF24' of SpltNPV-Pak-BNG and SpltNPV-G1 had a nucleotide sequence identity of 76%, which is slightly higher than reported (Breitenbach et al, 2013) for ORF24 of SpltNPV-G2 and its homolog ORF21 in SpliNPV-AN1956. Submolar bands were observed in the REN profiles of SpltNPV-Pak-BNG and SpltNPV-G1 DNA indicating mixed genotypes within the isolates analysed.…”

Section: Restriction Enzyme and Sequence Analysiscontrasting

confidence: 56%

“…The REN pattern of SpltNPV-Pak-BNG corresponds to the REN patterns of SpltNPV-Type-B (Kamiya et al, 2004) and Japanese SpltNPVs (Takatsuka et al, 2003;Takatsuka, Okuno, Nakai, & Kunimi, 2016) on the basis of visual inspection of the REN digests. On the other hand, the SpltNPV-G1 REN profiles are very different from SpltNPV-Pak-BNG, but they appear closely related to SpltNPVType-A (Kamiya et al, 2004) and SpliNPV- AN-1956(Breitenbach et al, 2013. Sequence analysis of the baculovirus core genes polyhedrin, DNApol, lef-6 and pif-2 (Table 6) supports this observation.…”

Section: Discussionmentioning

confidence: 70%

“…The SpltNPV-Pak-BNG nucleotide sequences of these genes were >98% identical to the sequence of SpltNPV-G2 (Pang et al, 2001), whereas the SpltNPV-G1 we researched was >99% identical to the SpliNPV-AN-1956 isolate (Breitenbach et al, 2013). The nucleotide identity of the polyhedrin, DNApol, pif-2 and lef-8 sequences of SpltNPVPak-BNG and SpltNPV-G1 were 94%, 87%, 89% and 88%, respectively (Table 6).…”

Section: Restriction Enzyme and Sequence Analysismentioning

confidence: 69%

“…The nucleotide identity of the 'ORF24'-like sequences of both viruses suggests that they are highly related and representative for SpltNPV-and SpliNPV-like viruses (Breitenbach et al, 2013;Pang et al, 2001). The smaller size PCR product of the 'ORF24'-like fragment with SpltNPV-G1 is due to a few gaps in the latter as compared to SpltNPV-Pak-BNG.…”

Section: Discussionmentioning

confidence: 99%

“…ORF24 and ORF21 appeared to be a unique homolog for SpltNPV (Pang et al, 2001) and SpliNPV (Breitenbach et al, 2013), respectively, and good markers for their identity. Two sets of primers, one for the SpltNPV-specific ORF-24 gene and one for the SpltNPVspecific polyhedrin gene (Pang et al, 2001), were used to confirm the identity of SpltNPV-Pak-BNG, SpltNPV-G1.…”

Section: Pcr Amplification Of Orf24mentioning

confidence: 98%

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Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Ali¹,

Werf

Vlak

2017

Biocontrol Science and Technology

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Spodoptera litura is an emerging insect pest in a wide range of crops worldwide. The insect is difficult to control because of resistance development to synthetic insecticides and emerging resistance to Bacillus thuringiensis toxins. Therefore, there is a need to develop biological control agents, preferably from an indigenous source to avoid risks associated with the importation of exotic natural antagonists. A Pakistani isolate of S. litura nucleopolyhedrovirus (SpltNPV, Baculoviridae), SpltNPV-Pak-BNG, was obtained from the field and characterized biologically and genetically, and compared to a SpltNPV reference isolate, SpltNPV-G1, thought to be of Chinese origin. The dose-mortality response (LD 50 ) of SpltNPVPak-BNG was not significantly different from that of the reference isolate SpltNPV-G1, but the time-to-death (LT 50 ) was significantly shorter for SpltNPV-Pak-BNG than for SpltNPV-G1. DNA restriction enzyme profiling indicated that SpltNPV-Pak-BNG and SpltNPV-G1 are different viruses. Sequence analysis of 'ORF24', specific for SpltNPV (and S. littoralis NPV as ORF21), and the conserved baculovirus core genes polyhedrin, DNApol, pif-2 and lef-8 confirmed that this was indeed the case and that SpltNPV-Pak-BNG is a genuine SpltNPV variant, whereas the SpltNPV-G1 isolate we used is, in fact, a SpliNPV variant, renamed to SpliNPV-G1. The newly isolated SpltNPV-Pak-BNG has the potential for development as a biocontrol agent of S. litura in Pakistan.ARTICLE HISTORY

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Section: Restriction Enzyme and Sequence Analysiscontrasting

confidence: 56%

Section: Discussionmentioning

confidence: 70%

Section: Restriction Enzyme and Sequence Analysismentioning

confidence: 69%

Section: Discussionmentioning

confidence: 99%

Section: Pcr Amplification Of Orf24mentioning

confidence: 98%

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Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Ali¹,

Werf

Vlak

2017

Biocontrol Science and Technology

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Partiti‐like viruses from African armyworm increase larval and pupal mortality of a novel host: the Egyptian cotton leafworm

Rice

et al. 2022

Pest Management Science

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BACKGROUND: The general principle of using microbes from one species to manage a different pest species has a clear precedent in the large-scale release of mosquitoes carrying a Wolbachia bacterium derived from Drosophila flies. New technologies will facilitate the discovery of microbes that can be used in a similar way. Previously, we found three novel partiti-like viruses in the African armyworm (Spodoptera exempta). To investigate further the utility and consistency of host shift of insect viruses as a potential pest management tool, we tested the interaction between the partiti-like viruses and another novel host, the Egyptian cotton leafworm (Spodoptera littoralis).RESULT: We found that all three partiti-like viruses appeared to be harmful to the novel host S. littoralis, by causing increased larval and pupal mortality. No effect was observed on host fecundity, and partiti-like virus infection did not impact host susceptibility when challenged with another pathogen, the baculovirus SpliNPV. Transcriptome analysis of partiti-like virus-infected and noninfected S. littoralis indicated that the viruses could impact host gene-expression profiles of S. littoralis, but they impact different pathways to the two other Spodoptera species through effects on pathways related to immunity (Jak-STAT/Toll and Imd) and reproduction (insulin signaling/insect hormones).CONCLUSION: Taken together with the previous findings in the novel host S. frugiperda, these results indicate a parasitic relationship between the partiti-like viruses and novel insect hosts, suggesting a possible use and novel pest management strategy through the artificial host shift of novel viruses.

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The complete genome sequence of a second alphabaculovirus from the true armyworm, Mythimna unipuncta: implications for baculovirus phylogeny and host specificity

et al. 2018

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Determination and analysis of the genome sequence of Spodoptera littoralis multiple nucleopolyhedrovirus

Cited by 22 publications

References 85 publications

Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Biological and genetic characterization of a Pakistani isolate of Spodoptera litura nucleopolyhedrovirus

Partiti‐like viruses from African armyworm increase larval and pupal mortality of a novel host: the Egyptian cotton leafworm

The complete genome sequence of a second alphabaculovirus from the true armyworm, Mythimna unipuncta: implications for baculovirus phylogeny and host specificity

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