In this work, hydrothermal treatment was assessed for the fractionation of industrial Eucalyptus globulus bark residue (EBR) to obtain biofuels and value-added compounds (such as oligosaccharides and phenolic compounds) in separated streams. Hydrothermal treatment was evaluated under non-isothermal regimen in the range of maximum temperature (T max) of 177-228 • C or severities (S 0) between 2.76 and 4.25. The highest oligosaccharides concentration (17.5 g/L) was achieved at S 0 of 3.69, corresponding to hemicellulose recovery of 77.30%. Under all severities evaluated in this work, over 90.94% and 84.17% of cellulose and lignin remained in the solid phase, respectively. The increase of S 0 improved 4.38-fold the enzymatic saccharification of cellulose, the highest glucose yield (84%) being achieved at S 0 of 4.04. Considering the maximal recovery of polysaccharides as glucose and oligosaccharides from the liquid and solid phases, S 0 of 4.04 was selected for bioethanol production using high solid loadings and following different strategies (simultaneous saccharification and fermentation-SSF and pre-saccharification and simultaneous saccharification and fermentation-PSSF). The utilization of 15% hydrothermally pretreated EBR without nutrient supplementation resulted in 26 g/L of ethanol, independently of the strategy used. An increase up to 17.5% solids and employing nutrient supplementation enabled the production of 38 g/L (or 4.8% v/v) of ethanol by PSSF.