Determinants of reovirus interaction with the intestinal M cells and absorptive cells of murine intestine

Wolf, Jacqueline L.; Kauffman, Robert S.; Finberg, Robert W.; Dambrauskas, Rina; Fields, Bernard N.; Trier, Jerry S.

doi:10.1016/0016-5085(83)90313-x

Cited by 152 publications

(74 citation statements)

References 26 publications

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“…Viruses that can be transported by M cells include reovirus (Wolf et al, 1983), poliovirus (Sicinski et al, 1990), human immunodeficiency virus type 1 (Amerongen et al, 1991), and mouse mammary tumor virus (Bevilacqua et al, 1989). Within the intestine, enzymatic digestion of native reovirus particles to intermediate subviral particles is necessary for uptake by M cells, presumably by revealing adherence sites (Amerongen et al, 1994).…”

Section: Cell Uptake Of Infectious Agentsmentioning

confidence: 97%

M Cells

Williams

Owen

2015

Mucosal Immunology

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Section: Cell Uptake Of Infectious Agentsmentioning

confidence: 97%

M Cells

Williams

Owen

2015

Mucosal Immunology

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“…In the gastrointestinal tract, reovirus gains entry via M cells and replicates in the lymphoid tissue of Peyer patches [20,21]. Whereas most of the gastrointestinal epithelium is protected by a thick glycoprotein layer, M cells within Peyer patches display unique glycosylation patterns.…”

mentioning

confidence: 99%

Reovirus Preferentially Infects the Basolateral Surface and Is Released from the Apical Surface of Polarized Human Respiratory Epithelial Cells

et al. 2008

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Mammalian reoviruses infect respiratory and gastrointestinal epithelia and cause disease in neonates. Junctional adhesion molecule-A (JAM-A) is a serotype-independent receptor for reovirus. JAM-A localizes to tight junctions and contributes to paracellular permeability in polarized epithelia. To investigate the mechanisms of reovirus infection of polarized epithelial cells, we assessed reovirus replication, release, and spread after apical and basolateral adsorption to primary human airway epithelial cultures. Reovirus infection of human airway epithelia was more efficient after adsorption to the basolateral surface than after adsorption to the apical surface, and it was dependent on JAM-A. Reovirus binding to carbohydrate coreceptor sialic acid inhibited apical infection, which was partially ameliorated by treatment of the cultures with neuraminidase. Despite the preference for basolateral infection, reovirus was released from the apical surface of respiratory epithelia and did not disrupt tight junctions. These results establish the existence of an infectious circuit for reovirus in polarized human respiratory epithelial cells.

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“…This observation indicates that the mechanism of M cell sampling of influenza viruses relates to a receptormediated transcytosis. Several viruses, including reoviruses, poliovirus, and HIV, have been observed to be transported efficiently across M cells of Peyer's patches by transcytosis [1,14,20]. Although the virological significance of the virus entry into M cells remains to be resolved, the results obtained in the present study imply that an influenza virus could easily pass through M cells and enter lymphoid cells and that the virus could cause a kind of viremia through the infected lymphoid cells.…”

Section: Discussionmentioning

confidence: 59%

The role of M cells of human nasopharyngeal lymphoid tissue in influenza virus sampling

et al. 2004

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Little is known about the role of the M cells of human nasopharyngeal lymphoid tissue in the sampling of viruses that cause respiratory infections. To clarify whether M cells could function as a gateway for influenza virus into human nasopharyngeal lymphoid tissue, excised adenoid tissue was incubated in media containing influenza A virus for 30, 60, and 90 min, respectively. Transmission electron microscopic observation revealed that many influenza viruses adhered to M cell surfaces and were taken up into the cytoplasmic vesicles of M cells after 30 min incubation; the viruses had been transported into enfolded lymphoid cells after 60 min incubation. By staining M cells with Sambucus nigra lectin, which specifically recognizes the NeuAcalpha2,6 Gal linkage of sialoprotein, it was also found that abundant receptors for the human influenza virus are present on the M cell surface. Our findings indicated that M cells of human nasopharyngeal tonsils function as a major port for influenza A virus entry and that the virus could be efficiently transferred to enfolded macrophages and lymphoid cells by M cells. The transport of influenza viruses to lymphoid cells by M cells may promote antigen delivery to the immune system, and these findings may be important for systemic delivery of those influenza viruses that have the capacity to productively infect cells outside of the respiratory tract.

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Determinants of reovirus interaction with the intestinal M cells and absorptive cells of murine intestine

Cited by 152 publications

References 26 publications

M Cells

M Cells

Reovirus Preferentially Infects the Basolateral Surface and Is Released from the Apical Surface of Polarized Human Respiratory Epithelial Cells

The role of M cells of human nasopharyngeal lymphoid tissue in influenza virus sampling

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